A strain of Escherichia coli with reduced membrane wall and its high-production phb application

A technology of Escherichia coli and recombinant bacteria, applied in the fields of genetic engineering and fermentation engineering, can solve the problems such as the inability of PHB to meet the needs of industrial production
CN110387347BActive Publication Date: 2021-03-02JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-03-02

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Abstract

The invention discloses an Escherichia coli membrane wall-reduced chassis strain and the application of the high-yielding PHB strain, belonging to the fields of genetic engineering and fermentation engineering. The present invention knocks out the core sugar gene cluster gmhD-waaQ and the O-antigen gene cluster wbbL-rmlB of the lipopolysaccharide on the wild-type Escherichia coli W3110 genome, the extracellular polysaccharide gene cluster galF-wza, and the type 4 capsular polysaccharide synthesis gene cluster yccC- ymcD, three flagellar gene clusters flhE-motA, fliY-fliR, flgN-flgL, and a pilus gene cluster fimB-fimH to obtain strain WJW08, and then transform the genes related to PHB synthesis into the strain to obtain recombinant strain WJW08 / pBHR68, Under normal fermentation conditions, the recombinant bacterium can synthesize 81.9% PHB of dry cell weight, which is 54.6 times that of the wild control bacterium W3110 / pBHR68 (1.5%).
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Description

technical field

[0001] The invention relates to an Escherichia coli membrane wall-reduced chassis strain and the application of the high-yielding PHB strain, belonging to the fields of genetic engineering and fermentation engineering. Background technique

[0002] Polyhydroxyalkanoates (PHA) are a class of renewable and degradable polymers with multiple material properties synthesized by microorganisms, and have broad application prospects in the fields of medicine, materials and environmental protection. Polyhydroxyalkanoate is widely present in microbial cells, mainly as a carbon source and energy storage carrier. The higher the ratio of C:N in the growth environment, the more favorable the synthesis of PHA. PHA exists in the form of hydrophobic particles in cells, and its content can exceed 90% of the dry weight of cells under certain conditions. According to different types of monomers and polymerization methods, PHA has a series of diverse material properties ranging ...

Claims

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