Tissue embedding solution and tissue slice preparation method

A technology for tissue sectioning and embedding fluid, which is applied in the field of pathological analysis and can solve problems such as insufficiency

Pending Publication Date: 2019-11-08
深圳市领先医疗服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing tissue embedding fluids are difficult to meet the needs of rapidly dev

Method used

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  • Tissue embedding solution and tissue slice preparation method
  • Tissue embedding solution and tissue slice preparation method
  • Tissue embedding solution and tissue slice preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1. Fix the bone tissue containing the metal implant in 10% formaldehyde solution by volume for 72 hours at room temperature;

[0069] 2. At normal temperature, dehydrate the sample obtained in step 1 in the ethanol aqueous solution with the volume fraction of 70%, 80%, 90%, 95%, 100% and 100% successively, and the dehydration time of each stage is 3 Hour;

[0070] 3. At room temperature, make the sample obtained in step 2 transparent twice in xylene, and the transparent time is 4 hours each time;

[0071] 4. At 0°C, use the first-stage permeate, the second-stage permeate, and the third-stage permeate to perform step-by-step infiltration treatment on the sample obtained in step 3, wherein the first-stage permeate includes formazan by volume ratio Hydroxyethyl acrylate 30mL, methyl methacrylate 55mL, butyl methacrylate 10mL and polyethylene glycol 400 5mL. The second-stage permeate is the same as the third-stage permeate, including that the first-stage permeate includes...

Embodiment 2

[0076] 1. Fix the bone tissue in 10% by volume formaldehyde solution for 24 hours at room temperature;

[0077] 2. At normal temperature, dehydrate the sample obtained in step 1 in the ethanol aqueous solution with the volume fraction of 70%, 80%, 90%, 95%, 100% and 100% successively, and the dehydration time of each stage is 3 Hour;

[0078] 3. At room temperature, make the sample obtained in step 2 transparent twice in xylene, and the transparent time is 4 hours each time;

[0079] 4. At 4°C, use the first-stage permeate, the second-stage permeate, and the third-stage permeate to sequentially perform step-by-step infiltration treatment on the sample obtained in step 3, wherein the first-stage permeate includes formazan by volume ratio. Hydroxyethyl acrylate 30mL, methyl methacrylate 56mL, butyl methacrylate 7mL and polyethylene glycol 4007mL. The second-stage permeate is the same as the third-stage permeate, including the first-stage permeate including 30 mL of hydroxyethy...

Embodiment 3

[0084] 1. Fix the bone tissue in 10% formaldehyde solution by volume for 12 hours at room temperature;

[0085] 2. At room temperature, dehydrate the samples obtained in step 1 in the ethanol aqueous solution with volume fractions of 70%, 80%, 90%, 95%, 100% and 100% successively, and the dehydration time of each stage is 6 Hour;

[0086] 3. At room temperature, make the sample obtained in step 2 transparent twice in xylene, and the transparent time is 6 hours each time;

[0087]4. At 2°C, use the first-stage permeate, the second-stage permeate, and the third-stage permeate to sequentially perform step-by-step infiltration treatment on the sample obtained in step 3, wherein the first-stage permeate includes formazan by volume ratio Hydroxyethyl acrylate 35mL, methyl methacrylate 55mL, butyl methacrylate 5mL and polyethylene glycol 4005mL. The second-stage permeate is the same as the third-stage permeate, including that the first-stage permeate includes 35 mL of hydroxyethyl ...

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Abstract

The invention relates to tissue embedding solution and a tissue slice preparation method. The tissue embedding solution comprises resin monomers, a plasticizer, a catalyzer, an accelerator and a defoamer; according to the volume percentage of the tissue embedding solution, the resin monomers include 30-35% of a hydrophilic resin monomer, 55-60% of a first hydrophobic resin monomer and 5-10% of a second hydrophobic resin monomer; the plasticizer is 5-8% in the volume percentage of the tissue embedding solution; the concentration of the catalyzer is 0.4g/100mL; the concentration of the accelerator is 40muL/100mL to 50muL/100mL; and the concentration of the defoamer is 200muL/100mL to 400muL/100mL. After being embedded by the tissue embedding solution, a tissue to be analyzed is sliced; and atissue slice difficult to deform can be prepared.

Description

technical field [0001] The invention relates to the technical field of pathological analysis, in particular to a method for preparing tissue embedding fluid and tissue slices. Background technique [0002] Tissue section is an important means for histopathological observation and pathological diagnosis. At present, there are two methods for preparing tissue sections: paraffin section and hard tissue section. With the rapid development of medical devices, the need for novel preparation methods for various implant-containing tissue sections has also increased. [0003] The paraffin section method is suitable for soft tissue sections, using paraffin as a tissue embedding medium. However, paraffin sectioning is not suitable for tissues containing implants, especially those containing metal implants and non-demineralized bone tissue. [0004] The hard tissue sectioning method is suitable for sectioning of hard tissues (eg, dental, orthopedic), using resin as an embedding agent...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/36G01N1/30C08F220/14C08F220/20C08F220/18
CPCG01N1/28G01N1/36G01N1/30C08F220/14
Inventor 张杰张贵王锦玉胡进
Owner 深圳市领先医疗服务有限公司
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