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Azotobacter chroococcum N24 with nitrogen-fixing effect and application thereof

A nitrogen-fixing and nitrogen-fixing bacteria technology, applied to nitrogen-fixing bacteria N24 and its application fields, can solve problems such as soil nitrogen deficiency cost and environmental pollution, and achieve the effects of increasing content, increasing crop yield, and promoting plant growth.

Active Publication Date: 2019-11-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Large-scale application of chemical fertilizers to solve the nitrogen deficiency problem in the soil is not only costly, but also causes environmental pollution problems

Method used

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  • Azotobacter chroococcum N24 with nitrogen-fixing effect and application thereof
  • Azotobacter chroococcum N24 with nitrogen-fixing effect and application thereof
  • Azotobacter chroococcum N24 with nitrogen-fixing effect and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The screening of embodiment 1 nitrogen-fixing bacteria

[0037] 1. Primary screening of nitrogen-fixing bacteria

[0038] In September 2017, wheat plants that had grown for about 7 months were collected from the farmland in Yangling District, Shaanxi Province, and the loosely bound soil at the roots of the wheat was shaken vigorously. Cut off the root of the plant and put it into a 10mL sterilized centrifuge tube, inject 5mL of sterile water and perform ultrasonic treatment to dissolve the rhizosphere soil closely combined with the root of the plant in the water. The collected soil samples are yellow loam, with 24.03mg / kg of available phosphorus, 126.17mg / kg of available potassium, 56.28mg / kg of alkaline nitrogen, 21.06g / kg of organic matter, and pH=8.27.

[0039] Make a series of 10-fold dilutions of the aqueous solution, and take 5 μL of the dilution to be 10 times -5 、10 -6 and 10 -7 The soil suspension was spread on Ashby medium and cultured at 28°C for 5 days. ...

Embodiment 2

[0048] Identification of embodiment 2 bacterial strain N24

[0049] 1. Morphological characteristics

[0050] The N24 strain was prepared into a bacterial suspension and spread on Ashby medium after dilution. After culturing at 28°C for 24 hours, the colonies and cell morphology were observed.

[0051] The results showed that the Gram staining of the bacteria was negative, the cells were oval, capsulated, and did not produce spores; after being cultured on Ashby medium for 24 hours, the colonies were round, with neat edges and translucent, and the colonies were slightly obvious at the later stage of growth. Brown.

[0052] 2. Physiological and biochemical characteristics

[0053] Refer to the bacterial identification manual to measure the physiological and biochemical characteristics of the strain N24, such as VP reaction, carbon source utilization, and indole test. The results show that the strain can use glucose, citric acid, and lactose as carbon sources for growth. Hydr...

Embodiment 3

[0058] Embodiment 3 wheat growth promoting test

[0059] Select wheat seeds with plump grains, sterilize the surface with 70% ethanol, rinse with sterile water at least 6 times, and apply the sterile water of the last rinse on the beef extract peptone solid medium to check whether the surface of the wheat is thoroughly sterilized. Sow the sterilized wheat seeds in the soil of the flower pot, plant 6 seeds in each pot, and cover the surface with about 1 cm of soil. Two treatments were set up in the experiment. In treatment 1, each plant was inoculated with 1 mL of sterile medium, and in treatment 2, each plant was inoculated with N24 bacterial solution (about 10 8 per mL) 1 mL, and each treatment was replicated 3 times. The flower pots are placed in the greenhouse, and the cultivation conditions are set as light 16h / 8h, temperature 25°C / 18°C. After the emergence of wheat seedlings, water was poured every 5 days, and plant height, dry weight and nitrogen content were measured ...

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Abstract

The invention belongs to the technical field of plant rhizosphere growth-promoting bacteria, and specifically discloses azotobacter chroococcum N24 with a nitrogen-fixing effect and application thereof. The azotobacter chroococcum N24 is deposited with the China Center for Type Culture Collection (CCTCC) on April 22, 2019 under the accession number CCTCC NO: M 2019281. The azotobacter chroococcumN24 is isolated from rhizosphere soil of wheat and identified by morphology, physiological and biochemical characteristics and genetics, and the azotobacter chroococcum N24 is identified as the azotobacter chroococcum. The strain can fix nitrogen in the atmosphere and can greatly increase the content of available nitrogen in the soil and increase the utilization rate of nitrogen in plants, so thatplant growth is improved, the use of chemical fertilizers is reduced, the crop yield is increased, and the azotobacter chroococcum N24 with the nitrogen-fixing effect is of great significance in agricultural production..

Description

technical field [0001] The invention relates to the technical field of plant rhizosphere growth-promoting bacteria, in particular to a nitrogen-fixing bacterium N24 with nitrogen-fixing effect and its application. Background technique [0002] Nitrogen is the mineral nutrient element with the largest demand for plants, and it is one of the nutrient elements necessary for the normal growth and development of plants. According to statistics, the nitrogen content of soil in my country is between 0.2 and 2g / kg dry soil, and most of the nitrogen content is below 1g / kg dry soil. Large-scale application of chemical fertilizers to solve the problem of nitrogen deficiency in the soil is not only costly, but also causes environmental pollution. Biological nitrogen fixation can provide about 60% of the world's available reduced nitrogen every year. It has great potential in solving the problem of nitrogen sources and is a long-term solution to the development of agricultural land prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P21/00C12R1/065
CPCC12N1/20C12R2001/065C12N1/205
Inventor 李哲斐王娟娟
Owner NORTHWEST A & F UNIV
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