Unlock instant, AI-driven research and patent intelligence for your innovation.

Matrix metalloprotease-sensitive injectable hydrogel

A technology of matrix metals and proteases, applied in medical preparations of non-active ingredients, medical science, skin diseases, etc., can solve the problems of poor comprehensive performance, injectable hydrogels cannot have both mechanical strength and degradation characteristics, and achieve Effects of mild reaction conditions, good biodegradability and biodegradability

Inactive Publication Date: 2019-11-15
GUANGDONG PROV MEDICAL INSTR INST
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the application of tissue repair, the existing injectable hydrogels cannot have both mechanical strength and degradation properties, and the comprehensive performance is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Matrix metalloprotease-sensitive injectable hydrogel
  • Matrix metalloprotease-sensitive injectable hydrogel
  • Matrix metalloprotease-sensitive injectable hydrogel

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] The preparation method of any one of the above-mentioned injectable hydrogels, comprising the following steps:

[0039] 1) Dissolve hyaluronic acid in pre-mixed DMF and water, adjust the pH, then add methacrylic anhydride dropwise, stir overnight, then adjust the pH, add absolute ethanol, and stand overnight at 4-8°C to make the methacrylic anhydride change The precipitation of hyaluronic acid is complete. Centrifuge to obtain a precipitate, dissolve the precipitate, dialyze and freeze-dry to obtain methacrylic anhydride-modified hyaluronic acid;

[0040] 2) Mixing methacrylic anhydride-modified hyaluronic acid and matrix metalloproteinase-sensitive polypeptide into a homogeneous solution, placing it in a syringe for injection molding, and obtaining a hydrogel after standing still.

[0041] Preferably, step 1) adjusts the pH to 8-9.

[0042] Preferably, the volume ratio of the methacrylic anhydride-modified hyaluronic acid and the matrix metalloproteinase-sensitive po...

Embodiment 1

[0048] (1) Preparation of methacrylic anhydride modified hyaluronic acid

[0049] Stir and dissolve 1 g of hyaluronic acid in 100 mL of mixed solvent (the volume ratio of DMF to water in the mixed solvent is 1:2). After fully dissolving, the temperature was cooled to 4°C in an ice-water bath, and the pH was adjusted to 8 with 5M sodium hydroxide. Then, 3.7 mL of methacrylic anhydride was slowly added dropwise, stirred overnight at 4° C., and the pH value was kept stable at 8. The overnight reacted solution was poured into 1 L of absolute ethanol, and stood overnight at 4° C. to completely precipitate the methacrylic anhydride-modified hyaluronic acid. Centrifuge and separate to obtain a precipitate, redissolve the precipitate in ultrapure water, dialyze through a dialysis bag with a molecular weight of 14,000, change the water every 4 hours, freeze-dry after 5 days of dialysis.

[0050] (2) Preparation of matrix metalloproteinase-sensitive degradable hydrogels

[0051] Prep...

Embodiment 2

[0060] (1) Preparation of methacrylic anhydride modified hyaluronic acid

[0061] 1g hyaluronic acid was stirred and dissolved in 100mL mixed solvent (the volume ratio of DMF and water in the mixed solvent was 2:3). After fully dissolving, the temperature was cooled to 4°C in an ice-water bath, and the pH was adjusted to 9 with 5M sodium hydroxide. 8 mL of methacrylic anhydride was slowly added dropwise, and stirred overnight at 4° C. to maintain a stable pH value of 9. The overnight reacted solution was poured into 1 L of absolute ethanol, and stood overnight at 4° C. to completely precipitate the methacrylic anhydride-modified hyaluronic acid. Centrifuge and separate to obtain a precipitate, redissolve the precipitate in ultrapure water, dialyze through a dialysis bag with a molecular weight of 14,000, change the water every 4 hours, and freeze-dry after 5 days of dialysis.

[0062] (2) Preparation of matrix metalloproteinase-sensitive injectable hydrogel

[0063] Prepare...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
compressive modulusaaaaaaaaaa
compressive modulusaaaaaaaaaa
Login to View More

Abstract

The invention discloses a matrix metalloprotease-sensitive injectable hydrogel. The hydrogel consists of methacrylic anhydride-modified hyaluronic acid and a matrix metalloproteinase-sensitive polypeptide. A preparation method comprises the steps of grafting methacrylic anhydride onto a hyaluronic acid macromolecular chain to obtain double-bond-modified hyaluronic acid, and then performing crosslinking with the matrix metalloproteinase-sensitive polypeptide containing two sulfhydryl groups to obtain the enzyme-sensitive hydrogel with an injectable property. The preparation method has the advantages of being simple in process and capable of completing preparation in one step. The raw materials have a good biological description property and are mild in reaction, and the obtained hydrogel has the mechanical property, degradability and the injectable property.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to an injectable hydrogel sensitive to matrix metalloproteinases. Background technique [0002] Hyaluronic acid (HA) is a glycosaminoglycan and one of the important components of the extracellular matrix. It has good biocompatibility and has the ability to regulate immunity and induce tissue regeneration, so it is widely used in biological in medical materials. Matrix metalloproteinases (matrix metalloproteinases, MMPs) are the general term for proteolytic enzymes that can be synthesized. They exist in many cells in the body such as smooth muscle cells and monocytes. MMPs can degrade extracellular matrix. [0003] Hydrogel is a kind of network polymer that swells in water and can retain water in the macromolecular void, and has a wide range of applications in biomaterials. Injectable hydrogel is a relatively special hydrogel, which generally has the characteristics of shear thi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00A61K9/06A61K47/36A61K47/42A61P17/02
CPCA61K9/06A61K47/36A61K47/42A61L26/0052A61L26/0061A61L26/008A61L26/009A61L2300/412A61L2400/06A61P17/02C08L5/08C08L89/00
Inventor 于珊刘群峰张丽
Owner GUANGDONG PROV MEDICAL INSTR INST