Strain for producing long-chain diacid and application thereof

The technology of long-chain dibasic acid and dibasic acid is applied in the field of fermenting and producing long-chain dibasic acid and strains producing long-chain dibasic acid, which can solve the problems of electric energy consumption, narrow temperature adaptation range and large personnel input, etc. Achieve the effect of reducing power consumption, reducing production cost and wide temperature adaptability

Active Publication Date: 2019-11-15
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the first aspect of the present invention is to provide a strain of Candida tropicalis to solve the technical problems that the current Candida tropicalis strains have a narrow temperature adaptability range and cannot achieve high-temperature fermentation, and then solve the problems of power consumption, equipment investment, and large personnel input. The resulting technical problems of high production costs

Method used

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  • Strain for producing long-chain diacid and application thereof
  • Strain for producing long-chain diacid and application thereof
  • Strain for producing long-chain diacid and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] Tropical Candida yeast (Candida tropicalis) 10468 of the present invention, through subculture experiment verification, there is no significant change in the colony morphology and the ability to produce long-chain dibasic acid after five subcultures (see figure 1 , figure 2 ). Under the condition of 35°C, the DC12 produced by the first-generation and sixth-generation strains were 171.40g / L and 171.65g / L respectively after shake flask fermentation; colony morphology: the surface of the colony was smooth and moist, milky white, shiny, round, with neat edges. It can be seen that the passage stability of Candida tropicalis 10468 of the present invention is good.

Embodiment 2

[0064] Take a glycerol tube seed and insert it into the YPD activation medium, cultivate for 36 hours, and then add the culture solution into the seed medium (cultivation is based on sterilization at 121°C for 20 minutes). Seed medium includes: sucrose 20g / L, corn steep liquor 2g / L, yeast extract 6g / L, potassium dihydrogen phosphate 8g / L, urea 2g / L, nC11 1mL; cultivated at 29°C for 48h, measured the seed liquid OD 620 Reached 0.8 (30 times of water dilution), the seed liquor is inoculated in the shaking flask that fermentation medium is housed, and fermentation medium comprises: sucrose 40g / L, corn steep liquor 5g / L, yeast extract 5g / L, dihydrogen phosphate Potassium 8g / L, potassium nitrate 4g / L, sodium chloride 1.5g / L, urea 0.5g / L. The n-alkane added to the fermentation medium was 1.5 mL nC11, and the fermentation medium was sterilized at 121° C. for 20 minutes. Fermented at 29°C. After the fermentation, the content of DC11 dibasic acid in the fermentation broth was determ...

Embodiment 3

[0066] Take a glycerol tube seed and insert it into the YPD activation medium, cultivate for 24 hours, and then add the culture solution into the seed medium (cultivation is based on sterilizing at 121°C for 20 minutes). Seed medium includes: sucrose 20g / L, corn steep liquor 4g / L, yeast extract 8g / L, potassium dihydrogen phosphate 12g / L, urea 1g / L, nC12 2ml; cultivated at 35°C for 48h, measured the seed liquid OD 620 Reached 0.6 (30 times of water dilution), the seed liquid is inoculated in the shaking flask that fermentation medium is housed, and fermentation medium comprises: sucrose 30g / L, corn steep liquor 3g / L, yeast extract 3g / L, dihydrogen phosphate Potassium 12g / L, potassium nitrate 7g / L, sodium chloride 1g / L, urea 1.5g / L. The n-alkane added to the fermentation medium was 3.5mL nC12, and the medium was sterilized at 121°C for 20 minutes. After the fermentation at 35°C, the content of DC12 dibasic acid in the fermentation broth was determined to be 172.53 g / L.

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Abstract

The invention relates to a strain, in particular to a strain for producing long-chain diacid. The invention further relates to a method for producing long-chain diacid by fermentation. The strain is candida tropicalis 10468, and the preservation number is CCTCC NO: M2017164. The strain is applied to fermentation of long-chain diacid. The method for producing the long-chain diacid is characterizedin that the long-chain diacid is produced by fermentation at the fermentation temperature of 29-41 DEG C with the candida tropicalis strain. The method solves the technical problems that a current candida tropicalis strain has a narrow temperature adaptation range and high-temperature fermentation cannot be realized, and thus the technical problems of high power consumption, equipment investment and personnel investment and high production cost caused by this are solved.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a bacterial strain producing long-chain dibasic acid, and also relates to a method for fermenting and producing the long-chain dibasic acid. Background technique [0002] Long-chain dibasic acid is an important raw material for synthesizing perfumes, nylon engineering plastics, hot melt adhesives, resins, cold-resistant plasticizers, medicines and pesticides, etc. Its structural formula is HOOC(CH2)nCOOH, where n is an integer greater than 7. Especially dodecanedibasic acid (DC12) and tetradecanedibasic acid (DC14), which are important raw materials for synthesizing high-grade nylon engineering plastics nylon 1212 and nylon 1414 with special properties and wide applications. Long-chain dibasic acids with more than 12 carbons do not exist in nature. The chemical synthesis route is long, the reaction requires high temperature and high pressure, and the requirements for catalysts are relatively...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/64C12R1/74
CPCC12P7/6409C12R2001/74C12N1/165
Inventor 汪江林李葳刘修才
Owner CATHAY R&D CENT CO LTD
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