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Analysis and Identification Method of Coumarin Components in Alcoholic Extract of Fistifolia

A technology for extracting coumarins and alcohols, which is applied in the directions of analytical materials, material separation, instruments, etc., can solve the problems of lack of systematic research on coumarin chemical components and pharmacological effects, and achieves high resolution and sensitive resolution. rate effect

Active Publication Date: 2020-01-03
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rao Gaoxiong and others separated and identified 11 compounds from the ethanol extract of Rhizoma lobata through column chromatography and infrared spectrophotometry, and only identified 6 kinds of coumarin chemical components. Systematic research and pharmacological action research of quasi-chemical components

Method used

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  • Analysis and Identification Method of Coumarin Components in Alcoholic Extract of Fistifolia
  • Analysis and Identification Method of Coumarin Components in Alcoholic Extract of Fistifolia
  • Analysis and Identification Method of Coumarin Components in Alcoholic Extract of Fistifolia

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Embodiment 1

[0031] In this embodiment, the method for analyzing and identifying the coumarin components in the alcohol extract of Lovola lobata comprises the following steps:

[0032] S1: Use the method of ethanol heating and ultrasonic extraction to extract the active ingredients in the medicinal material of schizophyllum, and prepare the test solution of syringa cypress; weigh the coumarin standard substance, and prepare the standard solution; the specific steps are:

[0033]S1.1 Preparation of the stock solution of the test product: crush the herbal medicine of schizofolia through a 120-mesh sieve, accurately weigh 5g, soak it in an ethanol solution with a volume concentration of 70% for 20min according to the solid-to-liquid ratio of 1:10, and then heat to At 45°C, use ultrasonic extraction for 1 hour, then filter to get the filtrate; repeat the above operation on the filter residue, combine the filtrate twice, concentrate until it has no alcohol smell, and obtain the extract; dilute t...

Embodiment 2

[0042] In this embodiment, the method for analyzing and identifying the coumarin components in the alcohol extract of Lovola lobata comprises the following steps:

[0043] S1: Use the method of ethanol heating and ultrasonic extraction to extract the active ingredients in the medicinal material of schizophyllum, and prepare the test solution of syringa cypress; weigh the coumarin standard substance, and prepare the standard solution; the specific steps are:

[0044] S1.1 Preparation of the stock solution of the test product: crush the herbal medicinal material of Herba chinensis through an 80-mesh sieve, accurately weigh 5g, soak it in an ethanol solution with a volume concentration of 70% for 60min according to the solid-to-liquid ratio of 1:12, and then heat to At 50°C, use ultrasonic extraction for 2 hours, then filter to get the filtrate; repeat the above operation on the filter residue, combine the filtrate twice, concentrate until it has no alcohol smell, and obtain the e...

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Abstract

The invention provides an analysis and identification method for coumarin components in a heracleummillefoliumethanol extraction solution, and belongs to the technical field of chemical component analysis. The method comprises steps as follows:S1, effective components in the heracleummillefoliummedicinal material are extracted with anethanol heating ultrasonic extraction method, and a heracleummillefolium test solution is prepared; a coumarin standard product is weighed, and a standard product solution is prepared; S2, the test solution and the standard product solution are detected with an UPLC (ultraperformance liquid chromatography)-quadrupole rod-TOF MS (time-of-flight mass spectrometer) method; S3, mass spectrometric data is analyzed by adopting PeakView1.2 software, and multiple coumarin components are rapidly identified from the heracleummillefolium ethanol extraction solution through rapid screening in a database and secondary mass spectrometry. 32 coumarin compounds are separated and identified from heracleummillefolium, relatively comprehensive and systematic analysis of coumarins in the heracleummillefolium is realized, and a foundation is provided for qualitative analysis of the coumarin components and pharmacological research of the heracleummillefolium.

Description

technical field [0001] The invention relates to the technical field of chemical component analysis, in particular to a method for analyzing and identifying coumarin components in an alcohol extract of unifoliate unifoliate. Background technique [0002] Coumarin is a secondary metabolite widely distributed in the plant kingdom, often associated with cinnamic acid, flavonoids, lignans, etc. In plants and microbial metabolites such as Fabaceae, Fabaceae, Orchidaceae, Solanaceae, Daphneaceae, Saxifragaceae and Oleaceae. Coumarin has various biological activities such as regulating plant growth, antibacterial and antiviral, anticoagulant, relaxing smooth muscle, absorbing ultraviolet rays and anti-radiation. So far, a variety of coumarin compounds have been isolated from citrus plants of Rutaceae. Heracleum millefolium Diels, a plant of the genus Umbelliferae, is a Tibetan medicine called Bamubao, also known as Qianye Duhuo and Tibetan Angelica. It is mainly distributed in my ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8679G01N2030/047
Inventor 严志宏邓敏芝蔡瑛袁恩周立分顿珠次仁刘苗苗
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE