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Method and system for processing biological sample

A technology for biological samples and samples, applied in the field of assisted reproduction, can solve the problems of reducing the fertilization ability of human spermatozoa, and the sample cannot be prolonged, and achieves the effect of reducing the skill level, reducing the need for instruments, and reducing exposure.

Pending Publication Date: 2019-11-19
GENEA IP HLDG PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are also disadvantages, for example, the sample cannot be left in the tube for an extended period of time due to its unfettered exposure to seminal plasma, which is known to reduce the fertilization capacity of human spermatozoa after ejaculation
[0064] Requires a variety of consumables;

Method used

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  • Method and system for processing biological sample
  • Method and system for processing biological sample
  • Method and system for processing biological sample

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Embodiment Construction

[0137] In general, the present invention addresses the problem of isolating motile sperm from a semen sample. In a preferred embodiment of the invention, semen is loaded into a first volume provided by a first fluid well. Sperm buffer solution is loaded into the second volume formed by the second fluid well, and a fluid communication interface in the form of a hole between the wells is opened such that motile sperm can swim through the hole into the second well. In another preferred embodiment, the present invention provides for opening the pores between the semen sample and the buffer solution for active transfer of motile spermatozoa into the buffer solution, simultaneously or at any given time during, before or after the separation process Spot the visual analysis of sperm entering the buffer chamber through the light path.

[0138] Figure 26 An overall comparison between an embodiment of the invention and a prior art DGC process is shown, which shows the advantageous pe...

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Abstract

The present invention is directed to the processing of a biological sample into its constituent components for use in ART and includes introducing a sample into a first volume disposed adjacent a second volume comprising buffer solution; wherein the first and second volumes are adapted for fluid communication therebetween; selectively separating the first volume from the second volume with a movable closure member disposed therebetween; wherein the step of selectively separating the first volume from the second volume comprises moving the closure member so that a fluid communication aperture is formed by one or a combination of the closure member or the closure member in combination with the first and second volumes to allow fluid communication between the first volume and the second volume such that motile cells migrate from the sample in the first volume to the buffer solution in the second volume.

Description

[0001] related application [0002] This application claims priority to Australian Provisional Patent Application No. 2017900270, filed on 31 January 2017 in the name of Genea IP Holdings Pty Ltd, entitled "Method and System for Processing a Biological Sample" rights, and its application documents are hereby incorporated by reference in their entirety for all purposes. technical field [0003] The present invention relates to the field of assisted reproductive technology (ART) and may be applicable to many fields including human reproduction, animal breeding, assisted reproductive technology (ART) research (human and animal) and sperm banking. In one form, the invention relates to methods, systems and devices for processing a biological sample into its constituent components for ART. The present invention is applicable to any biological sample comprising motile cells or organisms, and will be conveniently described hereinafter in relation to the processing and isolation of mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00B81B5/00C12N5/076B01L3/00
CPCC12M41/46C12M47/04A61D19/022B01L3/508B01L3/5082B01L2200/026B01L2200/0647B01L2200/0652B01L2300/0832B01L2300/0861A61B17/43B01L2300/04B01L2300/0663
Inventor 切斯特·亨德森本·霍布斯约翰·麦考马克本尼迪克特·斯图尔特-斯蒂尔安德鲁·C·詹金斯萨姆·加松爱德华多·欧姆苏珊娜·布兰迪泰亚·图利基·佩乌拉
Owner GENEA IP HLDG PTY LTD
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