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Stem cell repairing method and application

A stem cell and drinking water technology, applied in the field of cell repair, can solve the problems of reducing the concentration of free radicals and the limited application range of treatment technology, and achieve the effects of increasing dissolved oxygen content, safe and reliable stem cell growth and development, and inhibiting stem cell apoptosis.

Pending Publication Date: 2019-11-22
吴思远
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant applied for a Chinese invention patent entitled "An Improved Pulse Processor" on July 12, 1999 (the authorized announcement number is: CN1119289C). After setting and forming a specific pulse period, the far-infrared radiation wave formed by controlling the pulse period can reduce the concentration of free radicals in the food and achieve health care after the food is processed. However, the application range of this treatment technology is limited

Method used

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  • Stem cell repairing method and application
  • Stem cell repairing method and application
  • Stem cell repairing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Pulse instrument processor and working principle

[0038] A pulse processing instrument, including a pulse sequence generator 1, a drive circuit 2, an alarm circuit 3, a main CPU control unit 4, a power converter 5, a shift switch 6, a radiator 7, a processing platform 11 and a space storage area , the space storage area includes a space storage area 1 and a space storage area 2, the space storage area 1 and the space storage area 2 are arranged in parallel to ensure that the pulse wave incident angle is less than 90°, and the processing platform 11 is equipped with For ultrapure water, the pulse sequence generator 1 is composed of a high frequency oscillator a, a pulse storage frequency division circuit b and a frequency mixing circuit c, the high frequency oscillator a, a pulse storage frequency division circuit b and a frequency mixing circuit c connected in series; the pulse sequence generator 1 is connected with the radiator 7 through the drive circuit 2...

Embodiment 2

[0040] Embodiment 2 pulse processing instrument processing obtains the water of life

[0041] 2.1 Preparation of eau-de-vie

[0042] Preparation of Water of Life-1: The ultrapure water was treated twice with the pulse wave radiation generated by the improved pulse processor, each time for 30 minutes. The pulse wave was composed of several repetitive pulse sequences, and the repetitive pulse sequence consisted The first pulse sequence and the second pulse sequence are composed of four pulses and four intervals. The four pulses of the first pulse sequence have the same width, and the pulse width range is 1.3-3.5μs, the interval between the four pulses is the same, the pulse interval range is 0.7-3.1μs; the width of the four pulses of the second pulse sequence is equal, the pulse width range is 1.1-3.9μs, the first, The three pulse intervals are the same, the pulse interval range is 1.8-4.6μs, the second and fourth pulse intervals are the same, the pulse interval range is 0.9-2....

Embodiment 3

[0046] Example 3 H-MSC cell growth and proliferation experiment

[0047] 3.1 Grouping and training

[0048] H-MSC cells were recovered, passaged and expanded, and observed under a microscope to ensure that the cells were in good condition. After the cell confluence reached 80%, they were divided into 4 groups (normal group, control group, water of life-1 group and water of life-2 group) ), were inoculated in 6-well plates, and the seeding volume of each well was 1.2×10 5 , and the culture system was 2 mL / well.

[0049] 3.2 Cultivation

[0050] After 24 hours of cell inoculation, the medium was replaced for induction culture. The culture conditions were: H-DMEM medium + 10% FBS (BI fetal bovine serum) + 1% double antibody + 5% CO 2 +37°C; the H-DMEM medium in the normal group was commercialized H-DMEM medium (Coming 03318002, 201908); the medium in the control group was prepared by dissolving Gibco DMEM dry powder medium in ultrapure water; the water of life The -1 group me...

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Abstract

The invention belongs to the technical field of cell repair, and particularly relates to a stem cell repairing method and application. According to the invention, improvement is carried out on the basis of the original invention technology, and pulse wave treated water with the stem cell repairing effect is obtained through the pulse wave treated water with the pulse sequences with equal occupiedwidths and unequal intervals. On the basis of not influencing cell proliferation, a culture system prepared from the pulse wave treated water not only can reduce the natural apoptosis effect of humanumbilical cord mesenchymal stem cells (H-MSC), but also can play a role in inhibiting damage-induced H-MSC apoptosis, and has a cell repairing effect.

Description

technical field [0001] The invention belongs to the technical field of cell repair, and in particular relates to a method and application for repairing stem cells. Background technique [0002] Water is the source of life. In the original state, the water source is crystal clear and has a sweet taste. With the enrichment of time and materials, the environment becomes worse and worse. The original water source loses its original appearance, and the frequency of water sources being destroyed a huge change occured. Due to the high-rise buildings in the modern city, the interlacing of magnetic waves interferes with this frequency, and at the same time, the human biological frequency is disturbed and destroyed, resulting in pathological changes in the living body and forcing the emergence of diseases. [0003] At present, most of the existing coded pulse technologies are used in the electronics industry. The applicant applied for a Chinese invention patent entitled "An Improved...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/30C12N5/071C12N5/073C12N5/0735C12N5/0775C12N5/0797C02F103/04
CPCC02F1/30C12N5/0665C12N5/0623C12N5/0605C12N5/0602C12N5/0606C12N2500/05C02F2103/04C12N5/0668C12N2529/00C02F1/34C02F1/005C02F2103/026C02F1/487C12N2500/00
Inventor 吴思远王馨尉吴林泽
Owner 吴思远
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