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Detection method and computing method of genetic typing
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A detection method and genotyping technology, applied in the fields of genomics, microbial measurement/inspection, biochemical equipment and methods, etc., can solve problems affecting functions, expressions, differences, etc., and achieve the effect of small sample size
Pending Publication Date: 2019-11-22
SUZHOU EDEN MEDICAL LAB CO LTD
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SNP may affect the function and expression of its gene or associated gene, resulting in differences among individuals
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[0050] Rinse your mouth with drinking water 15 minutes before the saliva collection. Gargle 3-5 times, 5-10 seconds each time, to remove oral debris. Do not eat, drink, or chew gum after gargling. Take 200ul saliva plus 600ul sample preservation solution mixture.
[0054]c. Add all the above sample mixture (supernatant after centrifugation) into the adsorption column that has been loaded into the collection tube, centrifuge at 13000g for 1min, remove the waste liquid, and put the adsorption column back into the collection tube;
[0055] d. Add 500ul of washing buffer 1 (0.1M MOPS, pH6.5), centrifuge at 13000g for 1min, remove the waste liquid, and put the adsorption column back i...
Embodiment 2
[0090] Analyze and calculate according to the result that embodiment 1 obtains again:
[0091] The SNP sites used in the present invention mainly come from GWAS (Genome-wide Association Study, genome association analysis) research that has been reviewed and published, and the detection phenotypes include calcium, iron, zinc, selenium, magnesium and other mineral elements and proteins There are a total of 22 nutritional macromolecules such as fats, carbohydrates, etc., and 260 sites are detected. The SNP site must meet:
[0092] (1) Its frequency is not 1;
[0093] (2) The p value in at least one literature still maintains a significant correlation after Bonferroni correction, and the general GWAS research requires P<5X10-8;
[0094] References must meet at least any two of the following:
[0095] i. One literature sample population is Chinese; ii. One literature is from East Asian races other than Chinese; iii. Supported by two or more independent literatures; iv. One liter...
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Abstract
The invention relates to a detection method of genetic typing. The detection method comprises the following steps that saliva samples are taken, and nucleic acids in the samples are extracted and amplified; amplified nucleic acids are subjected to precipitation after enzymedigestion; the amplified nucleic acids are hybridized with bead chips after precipitation and heavy suspension, and the beadchips are cleaned after hybridization is conducted; the bead chips hybridized with sample DNA are subjected to single base extension and dyeing; and finally, Illumina Infinium LCG is used for scanningthe bead chips, and a result value is obtained. The invention further discloses a computing method of genetic typing. According to the computing method of genetic typing, genetic typing detection results are analyzed to obtain grades of required items; and the amount of required samples is small, the weight of a provided locus is unified and compared again, and obtained GPS is more accurate.
Description
technical field [0001] The invention belongs to the technical field of biological genotyping, and relates to a genotyping detection method and calculation method. Background technique [0002] The nutritional assessment of children is mainly to systematically observe and detect the nutritional status of their bodies, and then conduct a comprehensive and comprehensive assessment of the nutritional status. Commonly used indicators include: dietary survey, anthropometry, biochemical examination, and clinical examination. However, most of the above-mentioned methods are to indirectly or briefly test the status quo of human nutritional elements, and most of them are subjective evaluation methods, and there is no unified evaluation standard. Therefore, it cannot truly reflect the innate nutritional characteristics of the human body. In addition, a large number of studies have found that each child has individual differences in the absorption and metabolism of different nutrients...
Claims
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