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Immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein and preparation method thereof

A technology for glial and acidic proteins, applied in measuring devices, analytical materials, instruments, etc., can solve the problems of different antigenicity, achieve the effects of improving detection efficiency, high sensitivity, and not easy to reunite

Pending Publication Date: 2019-11-22
TIANJIN SAVANT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although peripheral hepatic stellate cells, chondrocytes, myoepithelial cells, fibroblasts, and lymphocytes also express GFAP, the antigenicity is different and it is not easy to be detected by GFAP monoclonal antibodies in the central nervous system

Method used

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  • Immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein and preparation method thereof
  • Immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein and preparation method thereof
  • Immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Preparation of GFAP immunochromatographic detection card

[0043] (1) Preparation of surface-activated fluorescent latex microspheres:

[0044] N,N'-dilauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolaurate, dodecylbenzenesulfonate and lauroyl glutamate, the weight ratio of the three is 0.5:4 :0.8:5.

[0045]①Take the surfactant (containing 0.5 mg of N, N'-dilauroyl ethylenediamine sodium diacrylate, 4 mg of polyethylene glycol monolaurate, 0.8 mg of dodecylbenzenesulfonate and 5 mg Lauroyl glutamate) was added to 0.2mol / L boric acid-borax buffer solution (containing 0.5wt%~3wt% PEG2000) at pH=8.4, then 1mL dimethylformamide, 1mg N,N '-Dicyclohexylcarbodiimide and 0.5mgN-hydroxysuccinimide were reacted at a stirring speed of 120r / min;

[0046] ②Take 1mL of fluorescent latex microsphere dispersion containing 1wt% carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10mL, 0.2mol / L, boric acid-borax buf...

Embodiment 2

[0081] 1. Preparation of GFAP immunochromatographic detection card

[0082] 1) Preparation of surface-activated fluorescent latex microspheres:

[0083]N,N'-dilauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolaurate, dodecylbenzenesulfonate and lauroyl glutamate, the weight ratio of the three is 3:5 :2:6.

[0084] ① Take the surfactant (containing 3 mg of N, N'-dilauroyl ethylenediamine sodium diacrylate, 5 mg of polyethylene glycol monolaurate, 2 mg of dodecylbenzenesulfonate and 6 mg of lauryl Acylglutamate) was added to 0.2mol / L, boric acid-borax buffer solution (containing 0.5wt%-3wt% PEG2000) at pH=8.4, and then 1mL dimethylformamide, 1mg N,N'- Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide were reacted at a stirring speed of 120r / min;

[0085] ②Take 1mL of fluorescent latex microsphere dispersion containing 1wt% carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10mL, 0.2mol / L, boric acid-borax...

Embodiment 3

[0115] 1. Preparation of GFAP immunochromatographic detection card

[0116] 1) Preparation of surface-activated fluorescent latex microspheres:

[0117] N,N'-dilauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolaurate, dodecylbenzenesulfonate and lauroyl glutamate, the weight ratio of the three is 4:7 :2.5:13.

[0118] ①Take the surfactant (containing 4mg of N,N'-dilauroyl ethylenediamine sodium diacrylate, 7mg of polyethylene glycol monolaurate, 2.5mg of dodecylbenzenesulfonate and 13mg of Lauroyl glutamate) was added to 0.2mol / L boric acid-borax buffer solution (containing 0.5wt%-3wt% PEG2000) at pH=8.4, then 1mL dimethylformamide, 1mg N,N' -dicyclohexylcarbodiimide and 0.5mgN-hydroxysuccinimide, reacted at a stirring speed of 120r / min;

[0119] ②Take 1mL of fluorescent latex microsphere dispersion containing 1wt% carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10mL, 0.2mol / L, boric acid-borax buffer solu...

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Abstract

The invention relates to an immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein. The immunochromatographic detection card comprises a test strip, wherein the test strip comprises a detection line and a quality control line, the detection line is coated with mouse-anti-human GFAP monoclonal antibodies, and the quality control line is coated with goat-anti-rabbit polyclonal antibodies. The immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein provided by the invention can be used for detecting serum, plasma and whole blood, and has important clinical significance for early judgment of degrees of brain injuries and prognosis of patients.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to an immunochromatographic detection card for rapidly detecting glial fibrillary acidic protein and a preparation method thereof. Background technique [0002] Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein that exists as a monomer. It has been found that there are 8 homologous isomers in humans, and the gene is located on the long arm of chromosome 17, zone 1, zone 1, including 9 exons and 8 introns, and its relative molecular mass is (40-53)* 103. GFAP mainly exists in the glial cells of the central nervous system, peripheral nervous system, and enteric nerve, and participates in the formation of the cytoskeleton and maintains its tension strength. Although peripheral hepatic stellate cells, chondrocytes, myoepithelial cells, fibroblasts, and lymphocytes also express GFAP, their antigenicity is different, and it is difficult to be detected by ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/533G01N33/543
CPCG01N33/558G01N33/533G01N33/54313
Inventor 林斯石岗卢年强
Owner TIANJIN SAVANT BIOTECH CO LTD
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