Fast and efficient propagation method for forming bulbils through in-vitro induction of stem segments of lilium lancifolium

A technology for lily dandelion and pearl buds, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high reproduction coefficient and the breeding cycle of dandelion buds easily affected by the environment, so as to improve the reproduction coefficient and shorten the cultivation period. Time, size uniform effect

Active Publication Date: 2019-11-26
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0006] Aiming at the advantages of the high reproductive coefficient of Lily Lily Buds and the disadvantages that the Lily Lilium Bud formation is easily affected by the environment and the breeding cycle is long under natural conditions, the present inve

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  • Fast and efficient propagation method for forming bulbils through in-vitro induction of stem segments of lilium lancifolium
  • Fast and efficient propagation method for forming bulbils through in-vitro induction of stem segments of lilium lancifolium
  • Fast and efficient propagation method for forming bulbils through in-vitro induction of stem segments of lilium lancifolium

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Embodiment 1

[0030] Embodiment 1 A kind of rapid and high-efficiency propagation method for in vitro induction of bulbil formation by lily lily lily stem section, comprising the following steps:

[0031] (1) Obtaining and surface sterilization of explants: the freshly harvested Julienne bulbs were embedded in the matrix and refrigerated in a 4°C cold storage until they germinated until the buds were 10 cm long ( figure 1 a), peel off the scales, cut off the stem buds from the bulb base with a double-sided blade, get 10cm long, and the stem buds wrapped by leaves ( figure 1 b) Wash away the substrate under running water, wash with detergent water for 10 minutes, rinse under running water until no foam is produced, then put it into a jar, and perform surface sterilization on an ultra-clean workbench. Surface sterilization: first sterilize the stem buds with 75% alcohol for 30 seconds, wash them once with sterile water, then sterilize them with 10% NaClO for 5 minutes, and finally wash them 4...

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Abstract

The invention provides a fast and efficient propagation method for forming bulbils through in-vitro induction of stem segments of lilium lancifolium. The method comprises the steps of (1) precooling treatment of seed bulbs; (2) elongation cultivation of the stems; (3) bulbil formation through induction of the stems, bulbil expansion and root taking. A lilium lancifolium stem elongation culture medium is prepared from MS, 30 g/L saccharose and 5.0 g/L agar; a bulbil induction, expansion and root taking culture medium is prepared from MS, 1.0 mg/L 6-BA, 30 g/L saccharose and 5.0 g/L agar. By means of the propagation method for forming the bulbils through in-vitro induction of the stems of the lilium lancifolium, each elongated stem can generate about 20 or more nodal axillae, each axillae can form 2-3 bulbils, the propagation coefficient of the lilium lancifolium is significantly increased, and the propagation efficiency of the lilium lancifolium is significantly improved. Compared withthe process of forming bulbils and directly inducing multiple shoots to re-generate bulblets through scales under a natural condition, the culturing period is greatly shortened, the steps are simplified, and the method has high practical application value.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, and relates to a rapid and high-efficiency propagation method for in vitro induction of bulbil formation by edible and medicinal Lilium lancifolium stem segments. Background technique [0002] Lilium lancifolium, also known as Yixing lily and tiger skin lily, is a perennial bulbous flower of the genus Lilium in the family Liliaceae (Long Yayi, Zhang Jinzheng, Zhang Lannian. Lily—the king of bulbous flowers. Beijing: Golden Shield Publishing House, 1999.). Its flowers are large and colorful, with strong resistance, and have high ornamental, edible and medicinal values. It is also an excellent parent of lily breeding (Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora of China. Vol. 14. Beijing: Science Press ,1980.; National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China. Beijing: China Medical Science and Technology Press, 2015.; Yu X R,...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 明军杨盼盼徐雷锋何国仁唐玉超
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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