Preparation method and application of cod protein peptide with high digestion and absorption characteristics

A cod protein, digestion and absorption technology, applied in the preparation method of peptides, chemical instruments and methods, protein composition of fish, etc., can solve the problems of waste of resources, environment, low additional output value, large amount of leftovers, etc., and achieve rich nutrition. , multi-commercial value, simple method effect

Pending Publication Date: 2019-12-03
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cod products include frozen fish fillets, fish segments, block frozen fish fillets, etc. During the processing process, a large amount of leftovers will be produced, and a large number of discarded by-products will cause waste of resources and environmental pollution.
At present, the enzymatic hydrolysis of leftovers has been widely studied by various countries, but it is mainly used for the production of animal feed, and the added value is not high.

Method used

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  • Preparation method and application of cod protein peptide with high digestion and absorption characteristics
  • Preparation method and application of cod protein peptide with high digestion and absorption characteristics
  • Preparation method and application of cod protein peptide with high digestion and absorption characteristics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation method of the cod protein peptide with high digestion and absorption characteristics in this embodiment is realized in the following steps:

[0043] S1, raw material pretreatment: peel off the leftovers of cod processing and take the meat to obtain minced cod meat, wash it, add deionized water according to the ratio of material to liquid 1:3 (w / v) g / ml, beat at 30000rpm for 5min, and obtain Raw material liquid;

[0044] S2, enzymatic hydrolysis: add 1mol / L NaOH solution to the raw material solution described in step S1 under stirring, adjust the pH to 8.0, add alkaline protease according to 0.1% of the weight of cod minced meat described in step S1, and heat in a water bath at 60°C And stir the enzymolysis, add 1mol / L NaOH solution at any time during the whole process to maintain the pH 8.0, and continue for 2 hours to complete the enzymolysis process, the resulting mixture is extinguished at 85°C for 15 minutes to obtain the enzymolysis solution; the ac...

Embodiment 2

[0049] The preparation method of cod protein peptide with high digestion and absorption characteristics in this embodiment is realized in the following steps:

[0050] S1. Raw material pretreatment: wash 800g of peeled cod meat, add 2400ml of deionized water, beat at 30000rpm for 5min, and obtain the raw material solution;

[0051] S2. Enzymolysis: add 1mol / L NaOH solution to the raw material solution in step S1 under stirring, adjust the pH to 8.5, then add 1.44g alkaline protease, heat and stir in a water bath at 45°C, and add 1mol / L NaOH solution at any time during the whole process / L NaOH solution maintained at pH 8.5, continued for 2 hours to complete the enzymolysis process, and the resulting mixture was inactivated at 85°C for 15 minutes to obtain about 3200ml of enzymolysis solution; the activity of the alkaline protease was 150,000 U / g;

[0052] S3. Centrifugation: Cool the enzymatic hydrolysis solution described in step S2 to 27°C, centrifuge at 4°C for 20 minutes a...

Embodiment 3

[0056] The preparation method of cod protein peptide with high digestion and absorption characteristics in this embodiment is realized in the following steps:

[0057] S1, raw material pretreatment: peel off the leftovers of cod processing and take the meat to obtain minced cod meat, wash it, add deionized water g / ml according to the ratio of material to liquid 1:3 (w / v), beat at 30000rpm for 3min, and obtain Raw material liquid;

[0058] S2, enzymatic hydrolysis: add 1mol / L NaOH solution to the raw material solution described in step S1 under stirring, adjust the pH to 8.5, add alkaline protease according to 0.2% of the weight of the minced cod meat described in step S1, and heat in a water bath at 60°C And stir the enzymolysis, add 1mol / L NaOH solution at any time during the whole process to maintain the pH8.5 constant, continue for 10h to complete the enzymolysis process, the resulting mixture is extinguished at 85°C for 15min, and the enzymolysis solution is obtained; the ...

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Abstract

The invention discloses a preparation method of cod protein peptide with high digestion and absorption characteristics. The method comprises the following steps: using cod meat as a raw material, carrying out pulping and alkaline protease enzymolysis, and carrying out membrane filtration and drying to obtain cod protein peptide powder. In the cod protein peptide powder prepared by the method, peptides with the molecular weight of 1000Da or below account for 90% or above of the protein content, are easy to digest and absorb by a human body and rich in nutrients and has various biological activities. The peptide with good digestion and absorption characteristics is developed by taking crushed cod meat as a raw material, cod processing byproducts are utilized, the price is low, more commercial values can be created, the method is simple and safe, and large-scale industrial production can be met. The prepared cod protein peptide has important significance in development and utilization offunctional foods, health foods or medicines.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method and application of cod protein peptide with high digestion and absorption properties. Background technique [0002] Protein is the material basis of all life, an important component of biological cells, the main raw material for human tissue renewal and repair, and plays a central role in biological systems. All life activities such as growth, development, exercise, heredity, and reproduction of the human body are inseparable from protein. As an indispensable nutrient for the human body, protein accounts for about 16.3% of human tissue, and about 3% of protein participates in metabolism to complete various physiological activities of the human body every day. If the intake of protein is insufficient for a long time, the normal metabolism, growth and development of the human body will not be able to proceed. In mild cases, diseases may occ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23J3/04A23J3/34A23L29/00A23L33/18A23L2/39A21D2/24A21D13/00A23L7/109A23L7/10A23L7/113C12P21/06C07K1/34A23C9/152A23C9/13A23G3/44
CPCA23J1/04A23J3/04A23J3/341A23L29/045A23L33/18A23L2/39A21D2/268A21D13/00A23L7/109A23L7/111A23L7/10A23L7/113A23L7/198C12P21/06C07K1/34A23C9/1526A23C9/1322A23G3/44A23V2002/00A23V2200/30A23V2250/55A23V2300/10
Inventor 杜明许诗琦涂茂林吴超王震宇马武超赵宁李国栋
Owner DALIAN POLYTECHNIC UNIVERSITY
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