Method for extracting coumarin from Pteris cretica L. var. laeta

A technology of phoenix fern and coumarin, which is applied in the direction of organic chemistry, etc., can solve the problems of application in the field of difficult medicine and low purity, etc.

Active Publication Date: 2019-12-03
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of the existing coumarin extraction method is low, and it is difficult to effectively apply it in the field of medicine

Method used

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  • Method for extracting coumarin from Pteris cretica L. var. laeta
  • Method for extracting coumarin from Pteris cretica L. var. laeta
  • Method for extracting coumarin from Pteris cretica L. var. laeta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Dried rough Pteris fern root (25kg) is pulverized, soaked in 125L 95% ethanol for 6 hours, refluxed at 120 degrees Celsius for extraction, repeated extraction 4 times, each time for 1 hour, the extract was recovered under reduced pressure, concentrated to obtain rough Pteris fern Crude root extract of fern.

[0022] (2) Disperse the crude extract of Pteris vulgaris in water, use 14 mesh-30 mesh polyamide resin column chromatography to elute, and carry out gradient elution with water and different concentrations of ethanol (30%, 60%, 95%) respectively , recovered under reduced pressure to obtain four parts of the eluate.

[0023] (3) Separation of the eluate extract at 30% of the site by medium-pressure preparative liquid chromatography under the condition that the volume solubility is 10%-80% methanol gradient elution, and 50 components are obtained, wherein the chromatographic column packing is ODS .

[0024] (4) After the 50 components prepared by medium pressur...

Embodiment 2

[0027] (1) Dried rough Pteris fern root (25kg) is pulverized, soaked in 125L 95% ethanol for 6 hours, refluxed at 120 degrees Celsius for extraction, repeated extraction 4 times, each time for 1 hour, the extract was recovered under reduced pressure, concentrated to obtain rough Pteris fern Crude root extract of fern.

[0028] (2) Disperse the crude extract of Pteris vulgaris in water, use 14 mesh-30 mesh polyamide resin column chromatography to elute, and carry out gradient elution with water and different concentrations of ethanol (30%, 60%, 95%) respectively , recovered under reduced pressure to obtain four parts of the eluate.

[0029] (3) Separation of the eluate extract at 30% of the site by medium-pressure preparative liquid chromatography under the condition that the volume solubility is 10%-80% methanol gradient elution, and 50 components are obtained, wherein the chromatographic column packing is ODS .

[0030] (4) After the 50 components prepared by medium pressur...

Embodiment 3

[0032] (1) Dried rough Pteris fern root (25kg) is pulverized, soaked in 125L 95% ethanol for 6 hours, refluxed at 120 degrees Celsius for extraction, repeated extraction 4 times, each time for 1 hour, the extract was recovered under reduced pressure, concentrated to obtain rough Pteris fern Crude root extract of fern.

[0033] (2) Disperse the crude extract of Pteris vulgaris in water, use 14 mesh-30 mesh polyamide resin column chromatography to elute, and carry out gradient elution with water and different concentrations of ethanol (30%, 60%, 95%) respectively , recovered under reduced pressure to obtain four parts of the eluate.

[0034] (3) Separation of the eluate extract at 30% of the site by medium-pressure preparative liquid chromatography under the condition that the volume solubility is 10%-80% methanol gradient elution, and 50 components are obtained, wherein the chromatographic column packing is ODS .

[0035] (4) After the 50 components prepared by medium pressur...

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Abstract

The invention discloses a method for extracting coumarin from Pteris cretica L. var. laeta, and belongs to the field of natural product extraction. The coumarin is extracted from Pteris cretica L. var. laeta through four steps of crude extraction of roots of Pteris cretica L. var. laeta, polyamide resin column chromatography separation, medium-pressure preparation of an eluate, and high performance liquid chromatography detection and preparation. According to the method, high performance liquid chromatography (HPLC) provided with a diode array detector (DAD) is adopted to quickly lock trace compounds from a chromatogram, so that trace compound single bodies are directionally prepared. The prepared coumarin is high in purity, and the content of the coumarin detected by high performance liquid chromatography can reach 95% or above.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a method for extracting coumarin from fern fern. Background technique [0002] Pteridophyllum rough belongs to Pteridaceae Pteridaceae, the whole plant of Pteridophyllum contains ergosterol, choline, tannins, glycosides and so on. The whole plant can be used as medicine, which has the effects of lowering blood pressure, deworming, and preventing cancer. Nephritis and lung diseases also have good curative effects, so it is called "longevity vegetable". Chinese medicine believes that Pteris fern is cold in nature and sweet in taste, enters the spleen, stomach, and intestine meridians, and has the effects of clearing away heat and reducing phlegm, lowering qi and smoothing the intestines, and strengthening the stomach. "Diet Therapeutic Materia Medica" claims that it can nourish the deficiency of the five internal organs, and also treat the diseases of qi bloc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/16
CPCC07D311/16
Inventor 吴娇李杨尹瀚林石玉生李春斌
Owner DALIAN NATIONALITIES UNIVERSITY
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