Method for extracting polysaccharide from overground parts of polygonatum cyrtonema

A technology of Rhizoma Polygonatum and its aerial parts, which is applied in the field of extraction technology of Rhizoma Polygonatum, can solve the problems of high production costs, achieve the effects of reducing supply prices, broadening supply channels, and increasing market supply

Inactive Publication Date: 2019-12-03
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are relatively high in production cost du

Method used

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  • Method for extracting polysaccharide from overground parts of polygonatum cyrtonema
  • Method for extracting polysaccharide from overground parts of polygonatum cyrtonema

Examples

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Embodiment 1

[0030] This embodiment is used to extract the polysaccharide of the aerial part of Polygonatum multiflora, and the specific process is as follows:

[0031] (1) Drying: collect the aerial part of Polygonatum multiflora, and dry it in an oven at 55°C until the water content does not exceed 10%.

[0032] (2) Pulverization: take the dried Rhizoma Polygonatum raw material, and pulverize it through a No. 4 sieve.

[0033] (3) Degreasing and impurity removal: Add 10 times the amount of petroleum ether (boiling range 60-90°C) to the Polygonatum polyphagoides powder, shake well, reflux in a water bath at 75°C for 2 hours, suction filter to recover petroleum ether, and evaporate the filter cake at the same time residual petroleum ether. After adding 10 times the amount of 95% ethanol solution to the filter cake to reflux for extraction for 1.5 h, the filter residue was collected by suction filtration and dried in an oven at 55°C.

[0034] (4) Extraction: Mix the filter residue of the ...

Embodiment 2

[0039] This embodiment is used to extract the polysaccharide of the aerial part of Polygonatum multiflora, and the specific process is as follows:

[0040] (1) Drying: collect the aerial part of Polygonatum multiflora, and dry it in an oven at 55°C until the water content does not exceed 10%.

[0041] (2) Pulverization: take the dried Rhizoma Polygonatum raw material, and pulverize it through a No. 4 sieve.

[0042] (3) Degreasing and impurity removal: Add 10 times the amount of petroleum ether (boiling range 60-90°C) to the Polygonatum polyphagoides powder, shake well, reflux in a water bath at 75°C for 2 hours, suction filter to recover petroleum ether, and evaporate the filter cake at the same time residual petroleum ether. After adding 10 times the amount of 95% ethanol solution to the filter cake to reflux for extraction for 1.5 h, the filter residue was collected by suction filtration and dried in an oven at 55°C.

[0043] (4) Extraction: Mix the filter residue of the ...

Embodiment 3

[0047] This embodiment is used to extract the polysaccharide of the aerial part of Polygonatum multiflora, and the specific process is as follows:

[0048] (1) Drying: collect the aerial part of Polygonatum multiflora, and dry it in an oven at 55°C until the water content does not exceed 10%.

[0049] (2) Pulverization: take the dried Rhizoma Polygonatum raw material, and pulverize it through a No. 4 sieve.

[0050] (3) Extraction: mix the above-ground part of Rhizoma Polygonatum with 15 times the amount of distilled water evenly, reflux extraction twice, each time for 2 hours, suction filter and collect the filtrate.

[0051] (4) Concentration and precipitation: the obtained filtrate is concentrated under reduced pressure at 60°C to 1 / 6 volume of the initial filtrate, and 95% ethanol is slowly added to the suction filtrate with constant stirring until the final concentration of ethanol is 80%, and placed at 4°C Centrifuge after 12 hours.

[0052] (5) Decolorization: Discard...

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Abstract

The invention discloses a method for extracting polysaccharide from overground parts of polygonatum cyrtonema. The method comprises the following steps: collecting overground parts of polygonatum cyrtonema, and crushing the dried polygonatum cyrtonema raw materials; adding petroleum ether into the obtained polygonatum cyrtonema powder, carrying out uniform shaking, then carrying out refluxing in water bath, carrying out suction filtration to recover the petroleum ether, adding an ethanol solution into filter cake, carrying out reflux extracting, then carrying out suction filtration, collectingthe filter residue, and drying the filter residue; uniformly mixing the filter residue of the overground parts of polygonatum cyrtonema with distilled water, carrying out suction filtration, and collecting the filtrate; slowly adding ethanol into the filtrate, continuously carrying out stirring, discarding the supernatant, collecting the precipitate, sequentially adding ethanol, acetone and diethyl ether into the precipitate, carrying out washing for multiple times, and carrying out drying by volatilizing; and dissolving the polysaccharide precipitate in distilled water, and repeatedly carrying out deproteinizing to obtain powder which is refined polysaccharide of polygonatum cyrtonema. Compared with the yield by single polysaccharide extraction of polygonatum cyrtonema rhizomes, the yield of the polygonatum cyrtonema polysaccharide can be increased by 40% by using the overground parts of polygonatum cyrtonema. Through applications of the device, polygonatum cyrtonema resources can bemore fully utilized, and the supply path of the polygonatum cyrtonema polysaccharide is also broadened at the same time.

Description

technical field [0001] The invention relates to a process for extracting Polygonatum polyflora, in particular to a method for extracting polysaccharides from aerial parts of Polygonatum polyflora. Background technique [0002] Polygonatumcyrtonema is a plant of the genus Polygonatum in the family Liliaceae, and it is included in the 2015 edition of "Chinese Pharmacopoeia" together with Polygonatum sibiricum and P. kingianum. In history, sealwort is a perennial herb with the same source of medicine and food. Its taste is sweet and flat, and it belongs to the spleen, lung and kidney channels. It has the effects of nourishing qi and nourishing yin, strengthening the spleen, moistening the lungs and benefiting the kidney. Modern studies have shown that Polygonatum is rich in polysaccharides, flavonoids, saponins, volatile oils and other components. The main component polysaccharides have the functions of lowering blood sugar and blood lipids, inhibiting bacteria, anti-inflammat...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 俞年军王秋丽韩荣春彭代银周安吴振东
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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