Photopolymerization gel for self-feedback of hardness distribution via color and preparation method and application thereof

A technology of hardness distribution and self-feedback, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators. Issues such as regulation or detection

Active Publication Date: 2019-12-06
SOUTHEAST UNIV
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main challenge in the control and detection of organ chips is that after the assembly is completed, it is a closed system, and conventional detection methods cannot regulate or detect the microenvironment inside the chip through the closed cover or substrate.
In order to realize the detection, it is necessary to design the interface of the detection system on the chip in advance. On the one hand, this increases the complexity of the chip. On the other hand, it is still difficult to detect the spatial distribution of each parameter in the microenvironment in this way.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Photopolymerization gel for self-feedback of hardness distribution via color and preparation method and application thereof
  • Photopolymerization gel for self-feedback of hardness distribution via color and preparation method and application thereof
  • Photopolymerization gel for self-feedback of hardness distribution via color and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Add acrylamide (7.00mg), Bis / PEGDA700 (0.08mg) and photoinitiator 2959 (0.08mg) to water-dispersed silica nanoparticles (90μL, Ф152nm, 30vol%); obtain a prepolymer solution;

[0035] 2) Ultrasonic treatment of the prepolymer solution obtained in 1) for 5 minutes;

[0036] 3) On the basis of 2), the pre-polymerization solution is evenly penetrated into the space between the two parallel glasses separated by capillary force, the gap thickness is 25 μm, and then exposed to the ultraviolet light of λ=365nm for 10s, 30s, 45s, 60s, 120s to photopolymerize the monomer; thus obtain hydrogel films of different colors and hardness;

[0037] 4) In order to increase cell affinity, gelatin was grafted onto the surface of the hydrogel membrane of 3). The hydrogel membranes were then sterilized in 75% ethanol in Petri dishes, in sterile PBS for at least 3 hours and in cell culture medium for 1 hour; the cell culture medium was removed and fresh cell culture medium was injected.

[...

Embodiment 2

[0040] 1) Add acrylamide (10.00mg), Bis / PEGDA700 (0.2mg) and photoinitiator 2959 (0.2mg) to water-dispersed silica nanoparticles (90μL, Ф152nm, 34vol%); obtain a prepolymer solution;

[0041] 2) Ultrasonic treatment of the prepolymer solution obtained in 1) for 5 minutes;

[0042] 3) On the basis of 2), the pre-polymerization solution is evenly penetrated into the space between the two parallel glasses separated by capillary force, the gap thickness is 50 μm, and then exposed to λ=365nm ultraviolet light for 10s, 30s, 45s, 60s, 120s to photopolymerize the monomer; thus obtain hydrogel films of different colors and hardness;

[0043] 4) In order to increase cell affinity, gelatin was grafted onto the surface of the hydrogel membrane of 3). The hydrogel membranes were then sterilized in 75% ethanol in Petri dishes, in sterile PBS for at least 3 hours and in cell culture medium for 1 hour; the cell culture medium was removed and fresh cell culture medium was injected.

[0044] ...

Embodiment 3

[0046] 1) Acrylamide (14.00mg), 7-acryloyloxyamino-4-methylcoumarin (2.00mg), Bis / PEGDA700 (0.45mg) and photoinitiator 2959 (0.45mg) were added to dimethyl ethylene Sulfone-dispersed silica nanoparticles (120 μL, Ф152 nm, 26 vol%); to obtain a prepolymer solution.

[0047] 2) Ultrasonic treatment of the prepolymer solution obtained in 1) for 5 minutes;

[0048] 3) On the basis of 2), the pre-polymerization solution is evenly penetrated into the space between two parallel glasses separated by capillary force, the gap thickness is 75 μm, and then exposed to λ=365nm ultraviolet light for 5 minutes to photopolymerize the monomer ; thereby obtaining a phototunable hydrogel film.

[0049] 4) To increase cell affinity, gelatin was grafted onto the surface of the hydrogel membrane. The hydrogel membranes were then sterilized in 75% ethanol in Petri dishes for at least 3 h in sterile PBS and 1 h in cell culture medium. Remove cell culture medium and inject new cell culture medium. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses photopolymerization gel for self-feedback of hardness distribution via color and preparation method and application thereof. The gel is used for cytoskeleton and the preparation of the gel comprises the following steps of preparing pre-polymerization liquid; pouring the pre-polymerization liquid in two glass sheets which are placed in parallel and irradiating the glass sheets by using 365 nm ultraviolet light for polymerization; and in the process, regulating and controlling the mechanical property of the gel by using 254 nm and 365 nm ultraviolet light so as to obtaina cytoskeleton, the mechanical property of which is regionally distributed. The hardness and distribution of the modified cytoskeleton are displayed in a color manner, and the hardness distribution onthe cytoskeleton is read in site through shooting a photograph. The method is applied to multiple cell culture systems and is capable of controlling the properties of micro environments in real timeand guiding the behaviors of the cells thereon in site. Under the condition of not opening a chip and not adding detection equipment, the mechanical parameters of the regulated and controlled cytoskeleton and the relationship between the cellbehaviors and the properties of the cytoskeleton are directly obtained through color analysis, so that large application potential is provided.

Description

technical field [0001] The invention relates to an intelligent cell scaffold that can be used for in-situ regulation and sensing of the microenvironment in an organ chip and a preparation method thereof, belonging to the field of biological materials, in particular to a photopolymerizable gel with color self-feedback hardness distribution and a preparation method thereof ,application. Background technique [0002] Organ-on-a-chip is an emerging technology that simulates the functions of human organs through three-dimensional culture of cells in vitro. Organ chips have broad application prospects in the fields of new drug research and development, disease models, personalized medicine and aerospace medicine. In 2016, organ chips were listed as one of the "Top Ten Emerging Technologies" by the World Economic Forum in Davos. One of the most challenging aspects of drug development is how to test the effectiveness and safety of drugs. Cell and animal experiments are currently ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 顾忠泽李森杜鑫
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap