Glutamine transaminase mediated cell membrane surface modification method

Abstract

The invention discloses a glutamine transaminase mediated cell membrane surface modification method. The glutamine transaminase mediated cell membrane surface modification method comprises the following steps: a modifier is linked to a substrate protein on the cell membrane surface of a target cell by glutamine transaminase, wherein the modifier contains glutamine residues, and the substrate protein contains lysine residues. The accurate and controllable cell membrane surface modification method based on natural enzymatic reactions is developed, and according to the platform, a new idea for regenerative medicine such as drug research and development, cell therapy and basic research is provided with cell function improvement as guidance, cell basic research and cell therapy as breakthrough,diversifying variability of functional fragments as media and simple operation, fast response and high repeatability as characteristics on the premise that physiological properties of cells are not changed.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a cell membrane surface modification method mediated by glutamine transaminase. Background technique [0002] Currently reported cell modification methods include: 1. Conditional stimulation; 2. Gene modification; 3. Cell membrane modification. [0003] Conditional stimulation mainly refers to the stimulation of cells through physical or chemical factors, so that cells respond to specific stimuli. Such as hypoxic culture, drug intervention, growth factor induction, etc., are beneficial for cells to up-regulate the expression of certain target molecules to achieve the purpose of changing cell characteristics. But such stimuli can lead to a series of uncontrollable changes in cells in terms of overall genes and epigenetics, so there are safety risks. For example, pre-stimulating cell differentiation will greatly improve its immunogenicity. If it is used for cell therapy, it ...

AI Technical Summary

Problems solved by technology

The most conventional method of cell membrane surface modification is mainly chemical method, but the process of chemical modification is very complicated, requiring multi-step operations to connect polypeptides to the cell membrane, which will affect the production of cell activity to varying degrees, and the most obvious The disadvantage is the randomness of the connection substrate

At present, the physical method is mainly based on the principle of similarity and compatibility between the cell membrane and the modifier, so that the modifier is embedded on the cell membrane. In this method, the treatment requirements of the modified object are relatively high, and after modification, it exists on the cell membrane for a long time, and the half-life is relatively short. long

However, the existing biological modification methods are mainly aimed at the specific enzyme molecules of a specific cell, which is not universal.

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