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Prussian blue microneedle electrode for blood glucose monitoring, preparation method thereof, blood glucose monitoring patch and preparation method thereof

A Prussian blue and blood sugar monitoring technology, applied in the field of biomedical engineering, can solve the problems of not knowing the continuous change of blood sugar, inconvenient detection of blood sugar content, wound infection, etc., to achieve continuous blood sugar monitoring, reduce psychological burden, and reduce pain.

Active Publication Date: 2019-12-13
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the mainstream way to monitor blood sugar is to prick finger bleeding with special test strips. In order to reflect changes in blood sugar, patients need to take repeated blood collections before and after meals. This process will bring great physical pain to patients. , it may also cause wound infection, and this measurement method cannot know the continuous change of blood sugar
Most of the blood glucose monitors currently on the market are not easy to carry, and it is very inconvenient for patients to detect blood glucose levels when they go out.

Method used

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  • Prussian blue microneedle electrode for blood glucose monitoring, preparation method thereof, blood glucose monitoring patch and preparation method thereof

Examples

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Effect test

preparation example Construction

[0044] The present invention also provides a method for preparing the above-mentioned Prussian blue microneedle electrode for blood glucose monitoring, comprising the following steps:

[0045] a) Gold microneedle electrodes are provided, and the gold microneedle electrodes include gold microneedle electrode bases and several gold microneedles whose bottoms are fixed on the gold microneedle electrode bases; then place the gold microneedle electrodes in Prussia In the blue deposition solution, a three-electrode constant potential method was used to deposit Prussian blue on the surface of the gold microneedle electrode to obtain a gold microneedle electrode with a Prussian blue layer deposited on the surface;

[0046] b) placing the gold microneedle electrode with the Prussian blue layer deposited on the surface in a chitosan solution mixed with glucose oxidase, using a three-electrode constant potential method to deposit chitosan and glucose oxidase on the surface of the Prussian...

Embodiment 1

[0085] This embodiment provides a figure 1 The blood glucose monitoring patch with the structure shown includes: a flexible substrate 1 , a working electrode 2 , an auxiliary electrode 3 and a reference electrode 4 . Among them, the flexible substrate 1 is a PDMS soft substrate with a thickness of 3 mm; the working electrode 2 is a chitosan-immobilized glucose oxidase-Prussian blue-gold microneedle array electrode; the auxiliary electrode 3 is a platinum microneedle array electrode; Electrode 4 is a silver / silver chloride microneedle array electrode.

[0086] The method for making the blood glucose monitoring patch provided in this embodiment is described below, and the specific steps are as follows:

[0087] First, a 4 μm silicon dioxide mask was grown on a 1 mm thick intrinsic silicon wafer by PECVD, and then the silicon wafer was subjected to oxygen etching surface treatment for 2 to 5 minutes by RIE. ~15min, which is to prepare for the subsequent photolithography. After...

Embodiment 2

[0096] Performance testing of blood glucose monitoring patch

[0097] 1) Instrument and reagent information:

[0098] CHI660 electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.);

[0099] 85-2 constant temperature magnetic stirrer (Jintan Chengdong Xinman Instrument Factory);

[0100] S2Pipette single-channel adjustable pipette (Sinopharm Chemical Reagent Co., Ltd.);

[0101] Phosphate buffer: 0.05mol / LKH 2 PO 4 +0.05mol / LK 2 HPO 4 +0.1mol / LKCl;

[0102] Glucose solution: 0.5mol / L;

[0103] All reagents were purchased from Sinopharm Chemical Reagent Co., Ltd.;

[0104] The experimental water was deionized water.

[0105] 2) Experimental process:

[0106] Firstly, the copper wire was mixed with silver glue and the chitosan-immobilized glucose oxidase-Prussian blue-gold microneedle array electrode, platinum microneedle array electrode and silver / silver chloride microneedle on the blood glucose monitoring patch prepared in Example 1 The array electrodes ar...

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Abstract

The invention belongs to the field of biomedical engineering, and particularly relates to a prussian blue microneedle electrode for blood glucose monitoring, a preparation method thereof, a blood glucose monitoring patch and a preparation method thereof. The prussian blue microneedle electrode provided by the invention comprises a gold microneedle electrode, wherein the gold microneedle electrodecomprises a gold microneedle electrode substrate and a plurality of gold microneedles, wherein the bottoms of the gold microneedles are fixed on the gold microneedle electrode substrate; a prussian blue layer compounded on the surface of the gold microneedle; and a chitosan immobilized glucose oxidase layer compounded on the surface of the prussian blue layer. According to the invention, the problem of tedious work caused by blood drawing test required for diabetes blood glucose monitoring at present is solved; the glucose concentration in the tissue fluid can be rapidly and accurately testedfor a long time only by penetrating the microneedle electrode provided by the invention through the cuticle of the skin, and the microneedle electrode is small in size and convenient to carry, and hasthe potential of serving as a good safe, portable and painless blood glucose electrochemical sensor.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a Prussian blue microneedle electrode for blood sugar monitoring, a preparation method thereof, a blood sugar monitoring patch and a preparation method thereof. Background technique [0002] With the improvement of living standards, the prevalence of diabetes is increasing year by year. If the blood sugar level of diabetic patients cannot be adjusted for a long time, many serious complications will be caused, so diabetic patients need to manage blood sugar level in daily life. Currently, the mainstream way to monitor blood sugar is to prick finger bleeding with special test paper. In order to reflect changes in blood sugar, patients need to take repeated blood collections before and after three meals. This process will bring great physical pain to patients. , It may also cause wound infection, and this measurement method cannot know the continuous change of bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/145
CPCA61B5/14514A61B5/14532A61B5/688A61B2562/164
Inventor 周成刚丁梦璠温晓镭魏珊珊
Owner UNIV OF SCI & TECH OF CHINA
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