Protein nano-film based on sulfhydryl and disulfide bond exchange reaction, and application thereof

A nano-membrane and exchange reaction technology, which is applied in the direction of peptide/protein components, medical preparations containing active ingredients, non-effective ingredients of polymer compounds, etc., can solve the problem of limited dual control ability of structure and function, lagging development of synthetic substitutes, Low film strength and other problems, to achieve excellent optical transparency, good chemical stability and mechanical stability, simple preparation method

Active Publication Date: 2019-12-13
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared to insoluble cellulose membranes, proteins or peptides exhibit excellent water solubility and safety. For natural examples, such as S layer cells, only rely on the mild water molecule glycoprotein supramolecular organization, but the assembly efficiency is low. , slow speed, limited ability to control both structure and function, and the development of synthetic substitutes lags behind; most of them are limited to structure and shape control, and the film strength is low, easy to disperse, and fragile, and needs to be cross-linked by glutaraldehyde to increase strength , toxic templates or extreme conditions to obtain silk fibroin or amyloid fibrils

Method used

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  • Protein nano-film based on sulfhydryl and disulfide bond exchange reaction, and application thereof
  • Protein nano-film based on sulfhydryl and disulfide bond exchange reaction, and application thereof
  • Protein nano-film based on sulfhydryl and disulfide bond exchange reaction, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] At room temperature, add 50 mg of L-cysteine ​​to 10 mL of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH of 7.4 and adjust the pH to 8 to 10 with 5 mol / L aqueous sodium hydroxide solution. Add lysozyme to 10mL of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH of 7.4, and then mix the two solutions evenly to obtain a mixed solution; drop the mixed solution on the surface of the glass slide or turn the glass slide upside down On the surface of the mixed solution droplet, a layer of protein nanofilm was clearly formed at the gas-liquid interface of the glass sheet or a layer of protein nanofilm was formed at the solid-liquid interface between the inverted glass sheet and the mixed solution droplet within 60 minutes.

Embodiment 2

[0024] At room temperature, add 70 mg of L-cysteine ​​into 10 mL of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH of 7.4 and adjust the pH to 8 to 10 with 5 mol / L aqueous sodium hydroxide solution. Add lysozyme to 10 mL of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH of 7.4, and then mix the two solutions evenly to obtain a mixed solution; add the mixed solution dropwise on the surface of the glass slide or place the glass slide upside down on the On the surface of the mixed solution droplet, a layer of protein nanofilm is clearly formed on the gas-liquid interface of the glass sheet within 60 minutes or a layer of protein nanofilm is formed on the solid-liquid interface between the inverted glass sheet and the mixed solution drop (see Figure 1~2 ).

Embodiment 3

[0026] At room temperature, add 100 mg of L-cysteine ​​into 10 mL of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH of 7.4 and adjust the pH to 8 to 10 with 5 mol / L aqueous sodium hydroxide solution. Add lysozyme to 10mL of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH of 7.4, and then mix the two solutions evenly to obtain a mixed solution; drop the mixed solution on the surface of the glass slide or turn the glass slide upside down On the surface of the mixed solution droplet, a layer of protein nanofilm was clearly formed at the gas-liquid interface of the glass sheet or a layer of protein nanofilm was formed at the solid-liquid interface between the inverted glass sheet and the mixed solution droplet within 60 minutes.

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PUM

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Abstract

The invention discloses a protein nano-film based on a sulfhydryl and disulfide bond exchange reaction, and an application thereof. The protein nano-film is obtained by reducing a disulfide bond-containing protein with a sulfhydryl-containing reducing agent and performing the exchange reaction on the sulfhydryl group and a disulfide bond. The nano-film has the advantages of preparation in a largearea, simple preparation method, achieving a mechanical strength without crosslinking, good stability, good optical permeability and good adhesion, and can be adhered to various base materials. The nano-film can be used for encapsulating molecules with different sizes to keep the activity of the molecules and realize controllable release.

Description

technical field [0001] The invention relates to a two-dimensional protein nano film with good mechanical strength prepared through the exchange reaction of thiol and disulfide bonds, which has the characteristics of being able to encapsulate and release active biomolecules of different sizes. Background technique [0002] Two-dimensional organic and biological thin films have attracted increasing research interest, and thin films with excellent physical and chemical properties hold great promise in electronics, energy, biosensors, and smart materials in recent years. However, compared to insoluble cellulose membranes, proteins or peptides exhibit excellent water solubility and safety. For natural examples, such as S layer cells, only rely on the mild water molecule glycoprotein supramolecular organization, but the assembly efficiency is low. , slow speed, limited ability to control both structure and function, and the development of synthetic substitutes lags behind; most of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L89/00C08K5/37A61K9/70A61K47/42A61K31/704A61K38/28
CPCC08J5/18A61K9/7007A61K47/42A61K31/704A61K38/28C08J2389/00C08K5/37
Inventor 杨鹏徐妍苏昊
Owner SHAANXI NORMAL UNIV
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