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isolated strain of the genus Streptochloa species T9 having high lipid production capacity and the use thereof

A technology of isolates, algae, applied in the direction of lipid/fat production, microorganisms, microorganisms, etc.

Active Publication Date: 2019-12-13
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the applicant is aware, there is no literature or patent record so far that has disclosed that algal oil produced by Scenedesmaceae sp. contains 1,3-diacylglycerol

Method used

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  • isolated strain of the genus Streptochloa species T9 having high lipid production capacity and the use thereof
  • isolated strain of the genus Streptochloa species T9 having high lipid production capacity and the use thereof
  • isolated strain of the genus Streptochloa species T9 having high lipid production capacity and the use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1. Isolation and screening of microalgalisolates

[0058] A. Source and isolation of test algal strains:

[0059] The applicant used a variety of seawater taken from different areas of Pingtung County (including seaside, Mipeng Bay and Houbi Lake, etc.) as sample sources to isolate microalgae isolates. First, add 10 mL of seawater sample and 30 mL of Wein's medium into a centrifuge tube (50 mL), and then place it in a centrifuge tube (50 mL), maintain a temperature of 25 ° C, and maintain an illuminance of 200-300 μmol / m 2 s and the light-dark cycle were set to 12 hours of light / 12 hours of darkness in an illuminated incubator (illuminated incubator) (model LTI-613-H, brand TKS), and then an appropriate amount of the obtained microalgae culture ( microalgal culture) were spread evenly on the Wein agar medium, and placed in the light incubator for cultivation. Afterwards, the applicant observed the morphology of algal colonies and the growth of algal strains on...

Embodiment 2

[0063] Example 2. Characterization of microalgae isolate T9

[0064] In order to confirm the algal species of the microalgae isolate T9 screened in the above Example 1, the microalgae isolate T9 was used for the following internal transcribed spacer (internal transcribed spacer, ITS) sequence analysis, lineage analysis (phylogenetic analysis), 18S rRNA sequence analysis and morphological characteristic analysis (analysis of morphological traits).

[0065] A. ITS sequence analysis:

[0066] Place an appropriate amount of fresh algae of the microalgal isolate T9 obtained in item A of the above Example 1 "Source and Isolation of Test Algae Strains" in a 2mL microcentrifuge tube, and use ZR fungi / bacteria DNA MiniPrep TM Kit (ZRFungal / Bacterial DNA MiniPrep TM kit) (Zymo Research) for the extraction of genomic DNA. The genomic DNA thus obtained was dissolved in an appropriate amount of sterile deionized water, thereby forming a sample containing the genomic DNA of the microalga...

Embodiment 3

[0098] Example 3. Preparation and Component Analysis of Algal Oil of Deutscheina sp. T9 Isolate

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PUM

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Abstract

The present invention relates to an isolated strain of the genus Streptochloa species T9 having high lipid production capacity and the use thereof. The invention discloses a Desmodesmus sp. T9 isolated strain with high lipid production capacity, and relates to an isolated strain of Desmodesmus sp. A deposit number BCRC 980048 is deposited in a biological resource preservation and research center (BCRC) of a food industry development and research institute (FIRDI); the invention relates to a method for preparing a CCTCC (China Center for Type Culture Collection) strain, which comprises the following steps of: registering the CCTCC strain in the China Center for Type Culture Collection (CCTCC) by using a registration number CCTCC M 2018152; the streptococcus species T9 isolate can be used for producing algal oil.

Description

technical field [0001] The present invention relates to a Desmodesmus sp. T9 isolate with high lipid-producing ability deposited with the Food Industry Development Institute (FIRDI) under deposit number BCRC 980048 The Biological Resources Conservation and Research Center (BCRC) of China, and the China Center for Type Culture Collection (CCTCC) under the deposit number CCTCC M 2018152. The Sesterella sp. T9 isolate can be used for the production of algal oil. Background technique [0002] Microalgae (microalgae) are a group of algae that can perform photosynthesis and are widely distributed in seawater and freshwater waters. Microalgae have the advantages of fast growth, good adaptability, and rich in essential proteins, lipids, polysaccharides, and carotenoids (carotenoids), so they have attracted attention from various fields, including the food industry, bioenergy industry, and biological Pharmaceutical industry, etc. In particular, compared with conventional oil crops...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64A23L29/00A23K20/158A23L33/115C11B1/10C10L1/02C12R1/89
CPCA23V2002/00A23K20/158A23L29/03A23L33/115C10L1/02C11B1/10C12P7/6463C12N1/125C12R2001/89A23V2250/20A23V2250/202
Inventor 黄英娥郭杨正黄政谕简美枝江尹玲尹韵慈董志宏朱燕华廖丽玲
Owner FOOD IND RES & DEV INST
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