A Trichoderma strain hb20111 for preventing and controlling soil-borne diseases of American ginseng and increasing yield and its application
A HB20111, soil-borne disease technology, applied in the application, pest control, fungicides and other directions, can solve the problems of quality decline, yield and quality decline, yield reduction, etc., and achieve remarkable results
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Embodiment 1
[0035] Isolation and Identification of Example 1 Trichoderma HB20111
[0036] Strain HB20111 is a soil sample collected from Binzhou Yellow River by using Trichoderma selective medium PDAm (PDA+300mg / L chloramphenicol+100mg / L streptomycin+20mg / L Rose Bengal+0.05%Triton X-100) obtained in isolation.
[0037]Weigh 10g of soil sample, put it into a saline Erlenmeyer flask with 90mL of 0.9% NaCl, 28°C, 160r / min, shake for 1h and mix thoroughly, take 1mL and mix it into a saline test tube containing 9mL of 0.9%NaCl, vortex to mix , followed by serial dilutions, and 1 mL of each gradient was added to a 90 mm Petri dish. Cool the PDA medium to below 60°C, add 300mg / L chloramphenicol, 100mg / L streptomycin, 20mg / L rose bengal and 0.05% Triton X-100, pour about 20mL medium into each culture dish, lightly Gently swirl and mix to disperse the samples in the culture dish evenly in the culture medium, culture at 28°C for 3-4 days, isolate and screen suspected Trichoderma strains for ident...
Embodiment 2
[0046] Embodiment 2 Trichoderma HB20111 antibacterial spectrum is measured
[0047] The pathogenic fungi are Rhizoctonia solani kuehn, Alternaria panax Whetz, Phytophthora cactorum, Pythium delaryanum Hess, Cylindrocarpon destructans, American ginseng cinerea, American ginseng anthracnose (Colletotrichum panacicola Uyeda et Takim), American ginseng root rot (Fusarium solani).
[0048] Use the confrontation test to measure the antibacterial spectrum of Trichoderma. The two ends of the PDA plate were inoculated with Trichoderma strains and pathogenic fungi with a diameter of 5 mm at a distance of 35 mm from the center. Inoculation of only pathogenic fungi was used as a control, and cultured at 25 ° C for 7 days. For slow-growing pathogens For fungi, pre-culture for 1-2 days and then inoculate Trichoderma confrontation culture, record the growth radius of pathogenic fungi in the confrontation direction, and calculate the inhibition rate.
[0049] Inhibition rate% = 100% × (r 0 ...
Embodiment 3
[0052] The preparation of embodiment 3 Trichoderma HB20111 inoculum
[0053] (1) Slant culture: use PDA medium, inoculate the strain HB20111 on the test tube medium, and culture at 28°C for 2-3 days;
[0054] (2) Erlenmeyer flask culture: use PDB medium, inoculate the test tube bacteria into the liquid Erlenmeyer flask, and place it on a shaker at 28°C for shaking culture for 1 day;
[0055] (3) Liquid culture: use liquid fermentation medium, sterilize at 115°C for 30 minutes, inoculate the bacteria into the fermenter, inoculum size 0.1% (v / v), culture at 28°C, ventilation rate 1:0.5-1.0, stirring speed 160r / min, culture 1-2d;
[0056] (4) Solid culture: use solid fermentation medium, sterilize at 121°C for 30 minutes, mix the liquid bacteria and solid medium evenly in the mixing tank, the inoculum size is 2-5% (v / v), transfer after inoculation In the solid culture room, the thickness of the culture medium is 3-4cm, the material temperature is controlled at 28±2°C, the room ...
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