Cyromazine detection method based on aptamer modified nanogold

A technology of nucleic acid aptamer and cyromazine, which is applied in the measurement of color/spectral characteristics, material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, etc., can solve the problem that cannot be obtained accurately Problems such as the content of cyromazine, to achieve the effect of fast detection speed, high sensitivity and low cost
CN110567952AActive Publication Date: 2019-12-13SHANGHAI INST OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF TECH
Publication Date
2019-12-13

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Abstract

The invention relates to a cyromazine detection method based on aptamer modified nanogold, and the method comprises the following steps: 1) adding a to-be-detected solution into a nanogold detection solution, and judging whether the detection solution changes color or not; 2) adding the to-be-detected solution into an aptamer modified nanogold detection solution, and judging whether the detectionsolution changes color or not; 3) adding the to-be-detected solution into a CTAB-containing aptamer modified nanogold detection solution, and judging whether the detection solution changes color or not; 4) carrying out absorbance test on the mixed solution of the to-be-detected solution and the CTAB-containing aptamer modified nanogold detection solution, and obtaining the content of cyromazine inthe to-be-detected solution according to the absorbance. Compared with the prior art, the method is simple and convenient to operate, can qualitatively judge melamine and cyromazine without a large instrument, can quantitatively detect the concentration of cyromazine within a certain range, and has the advantages of high detection speed, accurate detection result, good selectivity, low cost and the like.
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Description

technical field

[0001] The invention belongs to the technical field of analysis and detection methods, and relates to a method for detecting cyromazine based on nucleic acid aptamer-modified nano gold. Background technique

[0002] The chemical name of Cyromazine (Cyromazine, Cyr) is 2-cyclopropylamino-4,6-diaminotriazine, which belongs to triazine or The s-triazine compound is a high-efficiency insect growth inhibitor and parasite-killing insecticide, and is used as a feed additive in the process of livestock and poultry breeding. Studies have shown that most of the cyromazine entering the animal body is excreted through milk or feces in the form of the original drug or metabolites, and then exposed to the soil and water environment through livestock and poultry manure. Cyromazine in the soil or water environment re-enters the food chain through environmental fate, causing potential hidden dangers to organisms at different trophic levels and human health. Cyromazine is me...

Claims

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