Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cyromazine detection method based on aptamer modified nanogold

A technology of nucleic acid aptamer and cyromazine, which is applied in the measurement of color/spectral characteristics, material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, etc., can solve the problem that cannot be obtained accurately Problems such as the content of cyromazine, to achieve the effect of fast detection speed, high sensitivity and low cost

Active Publication Date: 2019-12-13
SHANGHAI INST OF TECH
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that thymine can combine with melamine, the metabolite of cyromazine, through hydrogen bonding, the nucleic acid aptamer that can specifically bind cyromazine has not been screened or synthesized, and when tested in the liquid When there may be interference of melamine, the content of cyromazine in the liquid to be tested cannot be accurately obtained

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cyromazine detection method based on aptamer modified nanogold
  • Cyromazine detection method based on aptamer modified nanogold
  • Cyromazine detection method based on aptamer modified nanogold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This example is used to investigate the impact of CTAB concentration on the detection system.

[0050] Prepare 0.25ppm and 0.5ppm cyromazine solutions respectively, mix with 0-100μM CTAB solutions of different concentrations, measure the absorbance at 650nm and 526nm wavelengths, and calculate the corresponding A 650 / A 526 , the result is as Figure 4 shown, and through A 650 / A 526 To determine the optimal CTAB concentration.

Embodiment 2

[0052] This example is used to investigate the effect of nucleic acid aptamer concentration on the detection system.

[0053] Prepare 0.25ppm and 0.5ppm cyromazine solutions respectively, and mix them with nucleic acid aptamers at different concentrations of 0-150nM, measure the absorbance at 650nm and 526nm wavelengths, and calculate the corresponding A 650 / A 526 , the result is as Figure 5 shown by A 650 / A 526 To determine the optimal nucleic acid aptamer concentration.

Embodiment 3

[0055] This embodiment is used to obtain the content of cyromazine in the test solution through the standard curve of cyromazine concentration and absorbance, and to check the reliability of the method.

[0056] 1) Add 3 mL of gold nanoparticles to a 5 mL centrifuge tube, and then add 5 μL of 10 4 nM nucleic acid aptamer solution, after 30min in water bath, add 3μL 10 3 nM CTAB to obtain the nucleic acid aptamer-modified nano-gold detection solution containing CTAB;

[0057] 2) Pretreatment of milk samples: In a 10mL centrifuge tube, add 3mL of 1% trichloroacetic acid and 3mL of milk samples, let stand for 10 minutes, then centrifuge at 10000r / min for 10 minutes, take the supernatant, and wash with 2.0mol / L NaOH to adjust the pH;

[0058] 3) Prepare a detection system with a known concentration of cyromazine: take 11 centrifuge tubes respectively equipped with an equivalent amount of nucleic acid aptamer-modified nano-gold detection solution containing CTAB, add different c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a cyromazine detection method based on aptamer modified nanogold, and the method comprises the following steps: 1) adding a to-be-detected solution into a nanogold detection solution, and judging whether the detection solution changes color or not; 2) adding the to-be-detected solution into an aptamer modified nanogold detection solution, and judging whether the detectionsolution changes color or not; 3) adding the to-be-detected solution into a CTAB-containing aptamer modified nanogold detection solution, and judging whether the detection solution changes color or not; 4) carrying out absorbance test on the mixed solution of the to-be-detected solution and the CTAB-containing aptamer modified nanogold detection solution, and obtaining the content of cyromazine inthe to-be-detected solution according to the absorbance. Compared with the prior art, the method is simple and convenient to operate, can qualitatively judge melamine and cyromazine without a large instrument, can quantitatively detect the concentration of cyromazine within a certain range, and has the advantages of high detection speed, accurate detection result, good selectivity, low cost and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection methods, and relates to a method for detecting cyromazine based on nucleic acid aptamer-modified nano gold. Background technique [0002] The chemical name of Cyromazine (Cyromazine, Cyr) is 2-cyclopropylamino-4,6-diaminotriazine, which belongs to triazine or The s-triazine compound is a high-efficiency insect growth inhibitor and parasite-killing insecticide, and is used as a feed additive in the process of livestock and poultry breeding. Studies have shown that most of the cyromazine entering the animal body is excreted through milk or feces in the form of the original drug or metabolites, and then exposed to the soil and water environment through livestock and poultry manure. Cyromazine in the soil or water environment re-enters the food chain through environmental fate, causing potential hidden dangers to organisms at different trophic levels and human health. Cyromazine is me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/33G01N21/78
Inventor 邢海波费雪莲党旭殷潘成
Owner SHANGHAI INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com