Strain separation, purification and identification method based on dried clitocybe squamulosa fruiting bodies

Abstract

The invention discloses a strain separation, purification and identification method based on dried clitocybe squamulosa fruiting bodies. The method comprises the following steps: rinsing and cleaninggill tissues of dried clitocybe squamulosa fruiting bodies with sodium pyrophosphate to prepare a spore suspension, adding the spore suspension into a Be culture medium for bacteriostasis, performingculturing at 25+ / -1 DEG C for 72 hours, carrying out microscopic examination under a 40-time objective lens to select germinated colorless transparent mononuclear hyphae, and transferring the mononuclear hyphae into a new Be bacteriostatic culture medium; dispersing and inoculating the small bacterial colonies with 5-6 germinating spores into a culture dish with a PDA (potato dextrose agar) culture medium, and continuing culturing to obtain pure clitocybe squamulosa mycelia; and then extracting the DNA of the clitocybe squamulosa, establishing an SCAR amplification system, and detecting a clitocybe squamulosa SCAR amplification product. The method effectively solves the problems that: in the pure strain domestication of existing dried clitocybe squamulosa fruiting bodies, the clitocybe squamulosa has small morphological difference from similar species and is not easy to distinguish by naked eyes, and the corresponding pure strain is difficult to obtain. The method has the characteristics of strong operability and simple purification and identification method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for strain separation, purification and identification based on dry fruiting bodies of the genus Pleurotus chinensis. Background technique [0002] The Taiwan mushroom in Wutai Mountain, Shanxi has a long-standing reputation, among which the scientific name of the Taiwan mushroom is Lincup Umbrella [ Clitocybe squamulosa (Pers.) Kumm], is one of the wild Taiwan mushrooms with extremely high nutritional value. The price of authentic dried wild Chrysanthemum mushrooms reaches thousands of yuan per 500 g. However, the acquisition and determination of pure strains is the primary task of domestication and cultivation. The morphological difference between Taiwan mushroom and its similar species is small, it is not easy to distinguish with naked eyes, it is difficult to obtain the corresponding pure strains, and it is difficult to identify them. Contents of the inven...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that there is little morphological difference between Taiwan mushroom and its similar species in the existing pure strains of dry fruiting bodies of Taiwan mushroom, it is difficult to distinguish with the naked eye, and it is difficult to obtain corresponding pure strains, the present invention discloses a The strain isolation, purification and identification method of the dry mushroom fruiting body has the characteristics of strong operability and simple purification and identification methods

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