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Method for simultaneously separating and purifying 6 flavonoid compounds from hibiseu manihot flowers

A technology for simultaneous separation of golden flower and sunflower, applied in the field of extraction and separation of natural compounds, can solve the problems of long production cycle, complicated operation, limited application of flavonoids, etc., and achieve the effects of short production cycle, high purity and simple operation.

Active Publication Date: 2019-12-20
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have the problems that the product is a mixture, the purity of monomeric flavonoids is low, the production cycle is long, and the operation is complicated, which limits the application of flavonoids in goldenflower sunflower.
At present, there is no report on the simultaneous purification of multiple high-purity flavonoids from sunflower sunflower

Method used

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  • Method for simultaneously separating and purifying 6 flavonoid compounds from hibiseu manihot flowers
  • Method for simultaneously separating and purifying 6 flavonoid compounds from hibiseu manihot flowers
  • Method for simultaneously separating and purifying 6 flavonoid compounds from hibiseu manihot flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1) Preparation of the extract of goldenflower sunflower: Precisely weigh 107g of dried goldenflower sunflower coarse powder, with a solid-liquid ratio of 1:20g / mL, 70% ethanol aqueous solution, 80°C as the extraction condition, microwave extraction for 10min, suction filtration, Repeat the extraction three times, collect the supernatant, and settle to a 250mL volumetric flask to obtain the goldenflower sunflower extract. Its high-performance liquid chromatogram is as follows: figure 1 shown;

[0053] 2) Solvent extraction: extract the sunflower extract obtained in step 1) with an equivalent amount of petroleum ether, ethyl acetate and n-butanol, repeat the extraction 3 times, combine the extracts, concentrate under reduced pressure, and set the volume to 250mL Volumetric flask, obtain Jinhua sunflower petroleum ether phase, ethyl acetate phase, n-butanol phase and water phase, the HPLC of its petroleum ether phase, ethyl acetate phase, n-butanol phase and water phase ar...

Embodiment 2

[0067] The basic operation is the same as in Example 1, the difference is that 110g of dried goldenrod sunflower coarse powder is accurately weighed, and after HPLC analysis, 35.4mg of rutin is obtained with a purity of 96.87%; 72.5mg of hyperoside is obtained with a purity of 95.37%; 67.5mg of isoquercitrin was obtained with a purity of 98.34%; 151.8mg of cottonsein-8-O-glucuronide was obtained with a purity of 99.27%; 63.2mg of myricetin was obtained with a purity of 96.58%; Cortex 25.3mg, purity 95.78%.

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Abstract

The invention relates to a method for simultaneously separating and purifying 6 flavonoid compounds from hibiseu manihot flowers, in particular to a method for simultaneously separating and purifyingrutin, hyperoside, isoquercitrin, hibifolin, myricetin and quercetin from hibiseu manihot flowers, and belongs to the fields of extraction and separation of natural compounds. The method comprises thesteps as follows: performing extraction on hibiseu manihot flowers by a microwave assisted ethanol aqueous solution, obtaining petroleum ether phase, ethyl acetate, n-butyl alcohol and aqueous phaseextracts, and realizing primary purification by use of different solubility of the 6 flavonoids of the hibiseu manihot flowers in each extract phase; and separating the mixture by polyamide rapid preparative chromatography and preparative high performance liquid chromatography to obtain the 6 flavonoid compounds including the rutin, the hyperoside, the isoquercitrin, the hibifolin, the myricetin and the quercetin with the purity higher than 95.0%. The method is simple to operate and shorter in production cycle and can be used as a preferred method for separating and purifying high-purity flavonoids from the hibiseu manihot flowers, and the prepared products have high purity.

Description

technical field [0001] The present invention relates to a method for separating and purifying flavonoids, in particular to simultaneously separating and purifying high-purity rutin, hyperin, isoquercitrin and cottoncetin-8-O-glucuronide from goldenflower sunflower , myricetin and quercetin methods. It belongs to the field of natural compound extraction and separation. Background technique [0002] Goldenflower sunflower is the dried corolla of the annual herb Hibiseu manihotL. of the Malvaceae genus Malvaceae. It contains a variety of active ingredients and has a good application prospect in the pharmaceutical, food or health care products industries. In recent years, as an important source of extracting flavonoids, sunflower has attracted the attention of all parties. Studies have shown that rutin, hyperoside, isoquercetin, myricetin and quercetin are the main flavonoids of goldenflower sunflower, and their contents are 0.0748%, 1.0763%, 0.5820%, 0.1185% and 0.0527% (Jou...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08C07D311/30C07D311/40
CPCC07D311/30C07D311/40C07H1/08C07H17/07
Inventor 孙立权张晓娇
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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