A drying method for hyaluronic acid

A drying method and technology of hyaluronic acid, applied in the field of biomacromolecule preparation, can solve the problems of difficult agglomeration, high water content of hyaluronic acid and its salts, and achieve the effects of enhancing stability, prolonging validity period and weakening hydrolysis reaction.

Active Publication Date: 2021-06-25
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of high water content of the current finished hyaluronic acid and its salt, the present invention provides a drying method for hyaluronic acid and its salt, the obtained product has low water content and is not easy to agglomerate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Add 2g of sodium hyaluronate (640,000 Daltons, loss on drying: 9.17%) to 500mL of normal saline to prepare a 4g / L sodium hyaluronate solution;

[0025] Prepare a mixture of hexamethyldisiloxane and absolute ethanol at a ratio of 5:95 (V / V) as organic solvent I containing 5% volatile silicone oil;

[0026] Slowly add 3 times the volume of organic solvent I to the sodium hyaluronate aqueous solution, and after standing still, discard the supernatant to obtain about 500 mL of precipitate;

[0027] (2) Prepare a mixture of hexamethyldisiloxane and absolute ethanol at a ratio of 50:50 (V / V) as organic solvent II containing 50% volatile silicone oil;

[0028] Take 2500mL of organic solvent II and add it to the precipitate in step (1), stir for 60 minutes, then let it stand and discard the supernatant to obtain the precipitate, repeat the above process 3 times, and filter the precipitate with filter cloth to obtain final sediment;

[0029] (3) Take the final precipitate ...

Embodiment 2

[0031] The experimental procedure is the same as in Example 1, except that the molecular weight is 150,000 Daltons, and the measured initial loss on drying of sodium hyaluronate is 7.09%; the measured loss on drying of the dried sodium hyaluronate obtained is 3.56%.

Embodiment 3

[0033] The experimental procedure is the same as that in Example 1, except that the molecular weight is 1.15 million Daltons, and the initial loss on drying of sodium hyaluronate is 10.67%; the dry weight loss on drying of the obtained sodium hyaluronate dry product is 4.27%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a drying method for hyaluronic acid, comprising the following steps: adding an organic solvent I containing volatile silicone oil to a solution containing hyaluronic acid, and discarding the supernatant after precipitation; The organic solvent II containing volatile silicone oil is washed to obtain a precipitate, and the product is obtained after drying. The volume fraction of the volatile silicone oil in the organic solvent I is 1%-10%; the volume fraction in the organic solvent II is 20%-60%. The hyaluronic acid drying method provided by the present invention is suitable for hyaluronic acid samples with different molecular weights and different concentrations. After drying by the method of the present invention, the weight loss on drying does not exceed 5%, and the residual organic solvents all meet the regulations.

Description

technical field [0001] The invention belongs to the field of biomacromolecule preparation and relates to a drying method for hyaluronic acid and its salt. Background technique [0002] Hyaluronic acid (HA), also known as hyaluronic acid, is composed of (1-β-4) D-glucuronic acid and (1-β-3) N-acetyl-D-glucosamine disaccharide units Chain-like polyanionic mucopolysaccharide composed of repeated links. With its unique molecular structure and physical and chemical properties, hyaluronic acid shows a variety of important physiological functions in the body, such as lubricating joints, regulating the permeability of blood vessel walls, regulating protein, water and electrolyte diffusion and operation, and promoting wound healing. More importantly, hyaluronic acid has a special water-retaining effect, and is the most moisturizing substance found in nature so far, and is known as an ideal natural moisturizing factor. [0003] Hyaluronic acid is very easy to absorb moisture. At pre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0072
Inventor 韩月梅李超杨盛林田晨颖刘建建郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap