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Fermentation method for producing eurotium cristatum

A coronoid bacterium and a fermentation method technology, applied in the production and fermentation field of coronoid bacterium, can solve the problems of single, simple medium composition, and insufficient efficacy of health care products, and achieve low raw material cost, controllable production process, The effect of simple production materials

Inactive Publication Date: 2019-12-20
青岛中科星熠高新技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most studies on the fermentation of S. coronoides generally have the defects of simple medium components, insufficient efficacy of related health products, and a single defect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1, taking 20 parts of dark tea, 60 parts of sorbifolium leaves and 5 parts of fucha according to parts by weight;

[0023] Step 2, put the three raw materials into water and boil for 1 hour, filter and collect the filtrate, which is called the pre-filtrate, adjust the pH of the pre-filtrate to 5.5-6.5 with vinegar, and refrigerate for later use;

[0024] Step 3, add vinegar to the tea dregs obtained in step 2, adjust the pH to 5.5-6.5, then dry the tea dregs to a humidity of 60%, then wrap them with three layers of medical gauze, put them in an enamel container, seal them, and keep at 15°C Under storage for 15 days, fermented tea dregs are obtained;

[0025] Step 4, adding purified water to the fermented tea dregs obtained in step 3 and boiling for 30 minutes, then filtering and collecting the filtrate, which is called post-filtrate;

[0026] In step 5, the back filtrate is mixed with the front filtrate to prepare a suspension of coronoid cysts.

[0027] Step 6, ...

Embodiment 2

[0031] Step 1, taking 40 parts of dark tea, 40 parts of sorbifolium leaves and 2 parts of Fu tea according to weight parts;

[0032] Step 2, put the three raw materials into water and boil for 1.5 hours, filter and collect the filtrate, which is called the pre-filtrate, adjust the pH of the pre-filtrate to 5.5-6.5 with vinegar, and refrigerate for later use;

[0033] Step 3, add vinegar to the tea dregs obtained in step 2, adjust the pH to 5.5-6.5, then dry the tea dregs to a humidity of 70%, then wrap them with three layers of medical gauze, put them in an enamel container, seal them, and keep at 117°C Under storage for 30 days, fermented tea dregs are obtained;

[0034] Step 4, adding purified water to the fermented tea dregs obtained in step 3 and boiling for 45 minutes, then filtering and collecting the filtrate, which is called post-filtrate;

[0035] In step 5, the back filtrate is mixed with the front filtrate to prepare a suspension of coronoid cysts.

[0036] Step 6...

Embodiment 3

[0040] Step 1, taking 35 parts of black tea, 45 parts of sorbifolium leaves and 4 parts of Fu tea according to parts by weight;

[0041] Step 2, put the three raw materials into water and boil for 1-2 hours, filter and collect the filtrate, which is called the pre-filtrate, adjust the pH of the pre-filtrate to 5.5-6.5 with vinegar, and refrigerate for later use;

[0042] Step 3, add vinegar to the tea dregs obtained in step 2, adjust the pH to 5.5-6.5, then air-dry the tea dregs until the humidity is 60-75%, then wrap them with three layers of medical gauze, put them in an enamel container, and seal them. Store at 15-20°C for 15-45 days to obtain fermented tea dregs;

[0043] Step 4, adding the fermented tea dregs obtained in step 3 to boiling for 30 to 60 minutes with purified water, and then filtering and collecting the filtrate, which is called post-filtrate;

[0044] In step 5, the back filtrate is mixed with the front filtrate to prepare a suspension of coronoid cysts.

...

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Abstract

The invention relates to the field of biochemical engineering, in particular to a fermentation method for producing eurotium cristatum. The method comprises the following steps: mixing tea leaves withactive additive components in a proportion to obtain a raw material, treating the raw material to obtain a pre-filtrate and first filter residues, treating the first filter residues to obtain fermented tea residues, treating the fermented tea residues to obtain a post-filtrate, mixing part of the pre-filtrate and the post-filtrate to obtain an eurotium cristatum spore suspension, mixing the residual pre-filtrate with carbon source nutrient substances to obtain a sterile culture solution, and inoculating the sterile culture solution with the eurotium cristatum spore suspension for fermentationtreatment to obtain the eurotium cristatum. Through five steps of pre-filtrate preparation, post-filtrate preparation, sterile culture solution preparation, eurotium cristatum suspension preparationand eurotium cristatum liquid fermentation, the method takes common tea leaves and pure natural substances such as Chinese wolfberry fruits, red dates and American ginseng as additives to prepare a fermentation culture solution. The production raw materials are simpler, the raw material cost is low, the production process is controllable, and industrial production can be completely achieved.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a method for producing and fermenting Saccharomyces coronoidis. Background technique [0002] In people's daily life, wolfberry, red dates, etc. are often used to make tea or soak medicinal wine. Studies have found that wolfberry contains a variety of amino acids, and contains special nutrients such as betaine, zeaxanthin, and physalis, which have very good health effects. The various functions of wolfberry mainly include immune regulation, anti-aging, anti-tumor, anti-fatigue, anti-radiation damage, regulating blood lipids, lowering blood sugar, lowering blood pressure, protecting the reproductive system, improving vision, improving respiratory disease resistance, beautifying and nourishing the skin, and moisturizing the skin , protect the liver, enhance hematopoietic function and so on. Jujube can improve human immunity and inhibit the growth of cancer cells, and the rutin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 梁栋
Owner 青岛中科星熠高新技术研究院有限公司
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