Establishment method of ratio electrochemical sensor of prostate-specific antigen detection and application thereof
A prostate-specific and construction method technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve high sensitivity, good reproducibility, and easy operation
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Embodiment 1
[0032] 1. The synthesis of materials, the specific method is as follows:
[0033] (1) Synthesis of Ag@RF NPs: 20 mL of 0.5 mg / mL resorcinol aqueous solution, 0.014 mL of 37% formaldehyde solution and 0.5 mL of 0.005 mol / L AgNO 3 Mix in a three-necked flask, heat to boiling at 90°C under magnetic stirring, then add 0.05mL of 2wt% ammonium hydroxide solution, continue to reflux for 20min, naturally cool to room temperature, centrifugally wash, and dry under vacuum to obtain Ag @RF NPs;
[0034] (2) Synthesis of Ag@RF-Ag NPs: Place the Ag@RF NPs prepared in step (1) in an ammonia environment, put 80 mg of dry Ag@RF NPs on clean filter paper, and place the filter paper on The etching reaction was carried out on a 20mL beaker containing 5mL ammonia water with a volume concentration of 20%. After standing for a certain period of time, the etched nanoparticles were dissolved in 5mL water to obtain Ag@RF-Ag NPs with a concentration of 16mg / mL. solution;
[0035] (3) Preparation of ...
Embodiment 2
[0041] 1. The synthesis of materials, the specific method is as follows:
[0042] (1) Synthesis of Ag@RF NPs: 30 mL of 0.5 mg / mL resorcinol aqueous solution, 0.014 mL of 38% formaldehyde solution and 1 mL of 0.01 mol / L AgNO 3 Mixed in a three-necked flask, heated to boiling at 100°C under magnetic stirring, then added 0.1mL, 2.5wt% ammonium hydroxide solution, continued to reflux for 30min, naturally cooled to room temperature, centrifugally washed, and dried under vacuum to obtain Ag@RF NPs, the particle size analysis of the synthesized nanomaterial Ag@RF NPs, the results are as follows figure 1 as shown ( figure 1 A is the particle size statistics of the Ag core in the synthesized nanomaterial Ag@RF NPs, figure 1 B is the particle size statistics of the synthesized whole nanomaterial Ag@RF NPs). from figure 1 In A, it can be seen that the average particle size of the Ag core is 18.02±4.50nm, and the thickness of the outer polymer RF shell is 44.7±4.0nm (Note: Ag@RF NPs c...
Embodiment 3
[0050] 1. The synthesis of materials, the specific method is as follows:
[0051] (1) Synthesis of Ag@RF NPs: 40 mL of 0.5 mg / mL resorcinol aqueous solution, 0.014 mL of 40% formaldehyde solution and 1.5 mL of 0.015 mol / L AgNO 3 The solution was mixed in a three-necked flask, heated to boiling at 110°C under magnetic stirring, then added 0.15mL, 3wt% ammonium hydroxide solution, continued to reflux for 40min, cooled naturally to room temperature, washed by centrifugation, and dried under vacuum to obtain Ag@RF NPs.
[0052] (2) Synthesis of Ag@RF-Ag NPs: Put the Ag@RF NPs prepared in step (1) in an ammonia atmosphere, put 120 mg of dry Ag@RF NPs on a clean filter paper, and put the filter paper in a container On a 20mL beaker with 15mL ammonia water with a volume concentration of 30%, the etching reaction was carried out, and after standing for a certain period of time, the etched nanoparticles were dissolved in 15mL water to obtain Ag@RF-Ag NPs with a concentration of 8mg / mL...
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