Application of chuanminshen violaceum or chuanminshen violaceum extract
A technology of Chuanming ginseng and its extract, which is applied in the application field of Chuanming ginseng or Chuanming ginseng extract, can solve problems affecting the quality of life of the elderly, and achieve the goal of delaying the related pathological process, reducing TG, and increasing HDL-C Effect
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Embodiment 1
[0033] Example 1: Preparation of Chuan Mingshen and Chuan Mingshen Extract
[0034] Chuanming ginseng used in the present invention is collected from Bazhong City, Sichuan Province, China, and dried to a moisture content of less than 8%.
[0035] The following steps are adopted in the present invention to extract Chuanming Ginseng:
[0036] S1: Take 5 kg of dried medicinal materials of Chuanming ginseng, cut into thin slices and pulverize into powder with a particle size of 40-60 mesh;
[0037] S2: Ultrasonic extraction of Chuanming ginseng powder with 6L of 95% ethanol, a total of 3 extractions, 1.5h each time; then filter the mixture, combine the filtrates and concentrate in vacuo to obtain about 300g of crude product extract;
[0038]S3: Purify by silica gel column chromatography (eluting with cyclohexane-ethyl acetate-methanol) to obtain about 10 g of refined Chuanmingshen extract (CEE, the main component is coumarin).
Embodiment 2
[0039] Embodiment 2: Experimental animal treatment
[0040] Fifty male New Zealand white rabbits (3 months old, with an initial weight of 1.7-2.1 kg) were provided by the Specialized Committee of Experimental Animals of Sichuan Province. They were raised in well-ventilated rabbit cages and provided with sufficient food and drinking water. The ambient temperature is controlled at 18-26°C, and the relative humidity is controlled at 30-70%. All animal experiments were carried out in accordance with the "Regulations on the Administration of Experimental Animals" formulated by the National Science and Technology Commission of China. The experimental rabbits were randomly divided into 5 groups (Table 1): the control group was given common rabbit feed, the model group was given customized high-fat feed, and the CEE-20 group, CEE-40 group, CEE-80 group and the positive control group were also given customized diet. High-fat diet, and oral administration of CEE at doses of 20 mg / kg / da...
Embodiment 3
[0047] Example 3: Investigation of Experimental Animal Health Indicators
[0048] 1. Serum Lipid Determination
[0049] At the beginning of the experiment and 4 weeks, 8 weeks, and 14 weeks after administration, fasting ear vein blood was collected, centrifuged at low temperature (2000 × g, 20 min, 4°C), and the serum was separated and the total cholesterol (TC ), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C) and triglyceride (TG) levels.
[0050] The effect of CEE on the serum lipids of experimental rabbits is as follows: Figure 1~4 As shown, the data in the figure are expressed in the form of mean±SD, * means compared with the model group, P﹤0.05, it can be seen that feeding high-fat diet significantly increased the blood lipid level of rabbits. Among them, the serum cholesterol level increased more than triglyceride, and successfully formed a hyperlipidemia model. Depend on figure 1 It can be seen that compared with the model group, CEE significantl...
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