Chili endophytic bacteria highly producing ironophore and application of chili endophytic bacteria to production increase of chilies

A technology of endophytic bacteria and siderophore, which is applied in the field of microorganisms to achieve the effect of promoting pepper growth, fast reproduction and remarkable effect.

Active Publication Date: 2019-12-24
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although there are many studies and reports on Pseudomonas, due to its aerobic characteristics, there ar

Method used

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  • Chili endophytic bacteria highly producing ironophore and application of chili endophytic bacteria to production increase of chilies
  • Chili endophytic bacteria highly producing ironophore and application of chili endophytic bacteria to production increase of chilies
  • Chili endophytic bacteria highly producing ironophore and application of chili endophytic bacteria to production increase of chilies

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Experimental program
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Effect test

Embodiment

[0049] (1) Sampling: The samples were collected from healthy pepper plants on the experimental field of the Agricultural Science and Vegetable Institute of Changsha City, Hunan Province.

[0050] (2) Separation and primary screening: cut off the main root and lateral root of pepper, wash the sediment with clean water, put it in an ultra-clean workbench to dry it, then put it in a disodium hydrogen phosphate solution and ultrasonically clean it for 1 minute to further remove surface impurities, and take out the root system in turn Use anhydrous ethanol, 4% sodium hypochlorite, and sodium thiosulfate for surface disinfection. After disinfection, use a large amount of sterile water to wash off the residual disinfectant. Take the last cleaning solution and apply it on an LB plate to test whether the disinfection is complete. Blow dry in the ultra-clean workbench; then put it into a sterilized mortar and grind it into a slurry, take 1 μl, 10 μl, 50 μl of the slurry to dilute and coa...

preparation example

[0054] The preservation number provided by the present invention is CCTCC No: M2019683. After the live cells of Pseudomonas nitroreducens PEB40 are activated, they are inoculated in LB liquid medium and cultivated at 28°C and 150rpm until they are in the logarithmic growth phase to obtain seeds. liquid; the seed liquid was inoculated into LB liquid medium with an inoculum volume of 2%, and cultured on a shaking table at 28°C and 150rpm for 40-48h to obtain a fermentation liquid; after pouring out the fermentation liquid, the plate was counted The final concentration of the fermented liquid is evaluated for the number of effective viable bacteria, and then the fermented liquid is diluted with water to obtain a bacterial concentration of 10 according to the evaluation result. 7 The solution of CFU / mL is the prepared bacterial agent.

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Abstract

The invention relates to the field of microorganisms, discloses chili endophytic bacteria highly producing ironophore and an application of the chili endophytic bacteria to production increase of chilies, and specifically provides the chili endophytic bacteria highly producing ironophore. The chili endophytic bacteria highly producing ironophore are pseudomonas nitroreducens PEB40, and the preservation number is CCTCC No: M2019683. The invention also provides a microbial inoculum. The microbial inoculum contains the chili endophytic bacteria highly producing ironophore. The invention also provides a method of increasing production of chilies. The method comprises the steps of enabling the chili endophytic bacteria highly producing ironophore and/or the microbial inoculum to be in contact with chili seedlings and/or chili plants. According to the strain provided by the invention, the microbial inoculum capable of promoting growth of chilies is prepared by a simple and quick operating method, so that the chilies treated by the microbial inoculum can be exuberant in vigor, and the strain is simple and convenient to use, low in cost and notable in effects, and besides, green, organic and pollution-free.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a capsicum endophytic bacterium with high siderophore production and its application in capsicum production increase. Background technique [0002] my country is the world's largest pepper producer and consumer, with an annual planting area of ​​about 1.33 million hectares. However, blind fertilization and excessive fertilization are common in pepper planting. The application of a large amount of chemical fertilizers leads to soil compaction and fertility decline, which can eventually cause The decline of pepper yield and quality has affected the sustainable development of agriculture. [0003] In order to solve a series of problems such as low fertilizer utilization rate and ecological environment deterioration caused by excessive application of chemical fertilizers, it is urgent to develop a new type of safe and effective fertilizer to replace some chemical fertilizers, and to deve...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06A01G22/05C12R1/38
CPCA01G7/06A01G22/05C12N1/20C12N1/205C12R2001/38
Inventor 雷平黄军黄彬彬毕世宇陈海荣郭照辉刘清术
Owner HUNAN INST OF MICROBIOLOGY
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