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Drying pretreatment and preparation method for improving extraction rate of cerebroside in lentinus edodes stems

A technology of Lentinus edodes and cerebroside, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low extraction rate, long time consumption, shortened extraction time, etc., and achieves high extraction rate and consumption. The effect of short time and good application prospects

Inactive Publication Date: 2019-12-27
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Directly extracting cerebrosides, the extraction rate is low or takes a long time. The present invention aims to provide a drying pretreatment and preparation method that shortens the extraction time and increases the extraction rate of cerebrosides in the stipe of Lentinus edodes, and promotes health food and scientific research. development in related fields

Method used

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  • Drying pretreatment and preparation method for improving extraction rate of cerebroside in lentinus edodes stems
  • Drying pretreatment and preparation method for improving extraction rate of cerebroside in lentinus edodes stems
  • Drying pretreatment and preparation method for improving extraction rate of cerebroside in lentinus edodes stems

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Embodiment

[0029] Pre-cool the fresh mushroom stalks at -4°C for 24 hours, and then use microwave / hot air for drying treatment. The microwave power is 400W, first use microwave drying, and run at intervals. The interval time is 30s, the operation time is 5s, and the microwave treatment time is 20min. Then add hot air to dry, the hot air temperature is 40 DEG C, until the water content of the mushroom stems is lower than 10%, after the drying is completed, the mushroom stems are crushed and sieved.

[0030] Take 5 g of shiitake mushroom stalk powder, add 100 ml of extract A, and extract A is an aqueous solution containing 5% methanol. Microwave / ultrasonic co-processing was performed for 20 minutes, the microwave power was 200W, the ultrasonic power was 250W, the ultrasonic time was 5s, and the ultrasonic interval was 5s. After the treatment, the centrifugation rate was 4000r / min for 10min, the supernatant was discarded, and the precipitate was collected.

[0031] According to the materia...

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Abstract

The present invention discloses a dry pretreatment and preparation method for improving extraction rate of cerebroside in lentinus edodes stems, and belongs to the field of food by-product processing.The method comprises the following steps: S1, pre-cooling fresh lentinus edodes stems; S2, drying the lentinus edodes stems using microwave / hot air until moisture content of the lentinus edodes stemsis less than 10%; S3, pulverizing and sieving the dried lentinus edodes stems to obtain lentinus edodes stem powder; S4, adding an extraction solution A into the lentinus edodes stem powder accordingto a material-liquid ratio A, using microwave / ultrasonic cooperative treatment, and then performing a centrifugation treatment A, pouring a supernatant A after the treatment and taking a precipitatefor a standby application; and S5, adding an extraction solution B to the precipitate according to a material-liquid ratio B, using an ultrasound treatment, then conducting a centrifugation treatmentB, taking out an obtained supernatant B after the treatment, and preparing a cerebroside-enriched sample. The established drying and extracting method for highly efficiently extracting cerebroside from lentinus edodes stems is short in time-consuming and high in extraction rate.

Description

technical field [0001] The invention belongs to the field of food by-product processing, and relates to a drying pretreatment and preparation method for improving the extraction rate of cerebrosides in shiitake stipe. Background technique [0002] Cerebroside, also known as acylceramide hexoside, is a kind of glycosphingolipid. It is a compound formed by binding a molecule of hexose to acylceramide with a glycosidic bond. [0003] Cerebrosides are structural components of cell membranes, mainly found in the animal kingdom, and also distributed in plants. Cerebrosides have various physiological activities such as anti-tumor and immune regulation. Mild action, less toxic and side effects, suitable for the prevention and treatment of some chronic diseases. [0004] The role of cerebrosides: 1. It can block the toxic effect of excitatory amino acids on brain cells, reduce the death of neurons, and have a significant protective effect on nerve cells; 2. Repair the regulation o...

Claims

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Application Information

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IPC IPC(8): A23L31/00A23L33/00A23L5/30C07H1/08C07H15/10
CPCA23L31/00A23L33/00A23L5/34A23L5/32C07H1/08C07H15/10A23V2002/00
Inventor 敬璞温心遥
Owner SHANGHAI JIAO TONG UNIV
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