Construction method and application of Sox30 knockout and restorable mouse animal model

A construction method and animal model technology, applied in the field of molecular biology, can solve the problems of pathology, unknown etiology, and difficulties in clinical treatment, and achieve the effect of short modeling time

Active Publication Date: 2019-12-27
ARMY MEDICAL UNIV
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Problems solved by technology

[0005] This application provides a Sox30 knockout and recoverable mouse animal model construction method and application to solve the existing pathology and unknown etiology of azoospermia and oligospermia in most NOA patients, and the clinical treatment is relatively difficult question

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  • Construction method and application of Sox30 knockout and restorable mouse animal model
  • Construction method and application of Sox30 knockout and restorable mouse animal model
  • Construction method and application of Sox30 knockout and restorable mouse animal model

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Embodiment Construction

[0041] The Sox family is a class of developmental nuclear transcription factors. It is a gene superfamily composed of genes related to the male sex determining gene SRY (sex determining region of Chr Y). Its members have a highly conserved DNA binding domain (HMG-box). Existing studies have shown that Sox genes are widely involved in embryonic development, sex determination, organ formation and tumorigenesis. Sox30 (SRY box containing gene 30) is the only member of Sox gene family H subfamily, which plays a very important role in sperm formation.

[0042] figure 1 Schematic flow chart of the method for constructing Sox30 knockout and recoverable mouse animal models in this application, such as figure 1 As shown, at first, the present application uses homologous recombination technology to knock the targeting vector (target vector) connected with the termination sequence element into the intron 1 (intron 1) of the wild-type mouse Sox30 gene (wild-type Sox30), wherein , intron...

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Abstract

The invention discloses a construction method and application of Sox30 knockout and restorable mouse animal model. A homologous recombination technique is adopted to conduct knockin of termination sequence elements, specifically-expressed Sox30 gene of mouse testes are knocked out, obtained F3 generation homozygous mice all show male specific infertility, that is, the morphology and pathological manifestation symptoms of the type of mice are consistent with Non-obstructive azoospermia (NOA) manifestation symptoms of human males, and the animal model can be used for study or simulation of an NOA disease. The mouse model constructed by the method can show the NOA disease at 45 days of birth, and the model constructing time is short. In addition, hybrid F3 generation male mice and F3 generation homozygous female mice can freely mate, a large number of surviving homozygous offsprings are generated, the homozygous offsprings can be subjected to reviving analysis by inducing and restoring expression of the knockout genes, the model constructing process is completed through natural reproduction, and other operation is not needed in the model constructing process, so that the model constructing process is simple and low in cost, and wide using and promotion are convenient.

Description

technical field [0001] The present application relates to the technical field of molecular biology, in particular to a method for constructing a Sox30 knockout and recoverable mouse animal model and its application. Background technique [0002] As an important disease affecting human reproduction, infertility has become a serious medical health problem and a serious social problem worldwide, and has attracted widespread attention. The World Health Organization (WHO) predicts that infertility will become the third largest disease after tumors and cardiovascular diseases among diseases affecting human life and health. According to the latest statistics from WHO, the number of infertile couples worldwide is about 80 million, of which male patients account for about 50%, and the proportion of infertility caused by males is increasing year by year. In my country, with the opening of the national "two-child" policy, the problem of male infertility is particularly prominent. The...

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Application Information

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IPC IPC(8): C12N15/85A01K67/027A01K67/02
CPCC12N15/8509C07K14/47A01K67/0276A01K67/02A01K2207/15A01K2217/075A01K2227/105A01K2267/03
Inventor 韩飞刘晋祎刘文斌姜晓曹佳
Owner ARMY MEDICAL UNIV
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