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Method for detecting heterocyclic amines in grease

A technology for heterocyclic amines and oils, applied in the detection field of heterocyclic amines, can solve the problems of carcinogenicity, mutagenicity, pollution, etc., and achieve the effect of short time consumption and simple operation.

Inactive Publication Date: 2019-12-27
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, during the processing of oil from oilseeds to finished oils, it is easy to be polluted by heterocyclic amine compounds, which are carcinogenic and mutagenic (Sanz-Alaejos, M., & Afonso, A.M.(2011).Factors that affect the content of heterocyclic aromatic amines in foods. Comprehensive Reviews in Food Science and Food Safety, 10, 52-108. De Meester, C. (1995). Genotoxic potential of beta-carbolines: A review. Mutation Research, 339, 139-153.)
The existence of heterocyclic amines increases the risk factors of oil, reduces the safety, and the safety of oil is questioned by people, which is contrary to the growing awareness of food safety of Chinese people.
[0003] The existing methods are all focused on the detection of heterocyclic amines in meat products, coffee products and alcoholic beverages, and have not yet been applied to the detection of heterocyclic amines in oils

Method used

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  • Method for detecting heterocyclic amines in grease
  • Method for detecting heterocyclic amines in grease
  • Method for detecting heterocyclic amines in grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] After placing 2 g of soybean oil in a polypropylene centrifuge tube, add 40 μL of internal standard working solution (4,7,8-TriMeIQx solution, concentration: 1.0 μg / mL) and 10 mL of 1% ammonia acetonitrile solution in sequence, vortex for 5 min, and sonicate for 10 min , centrifuge at a speed of 3500r / min for 5min to obtain the first supernatant and residue;

[0082] The residue was repeatedly extracted once according to the above steps to obtain a second supernatant;

[0083] After mixing the first supernatant and the second supernatant, add 10 mL of saturated n-hexane solution of acetonitrile for degreasing to obtain a degreased extract solution (10 mL);

[0084] The extraction solution after the degreasing is loaded (sample loading speed is 1mL / min) in advance with 3mL methanol, the PCX solid-phase extraction column activated by 3mL ultrapure water, then with 3mL0.1mol / L hydrochloric acid and 3mL methanol Rinse the small column, drain it after rinsing, and elute wit...

Embodiment 2

[0096] After placing 2 g of soybean oil in a polypropylene centrifuge tube, add 40 μL of internal standard working solution (4,7,8-TriMeIQx solution, concentration: 1.0 μg / mL) and 10 mL of acetonitrile solution in sequence, vortex for 5 min, sonicate for 10 min, 3500r Centrifuge 5min under the rotating speed of / min, obtain the first supernatant and residue;

[0097] The residue was repeatedly extracted once according to the above steps to obtain a second supernatant;

[0098] After mixing the first supernatant and the second supernatant, add 10 mL of saturated n-hexane solution of acetonitrile for degreasing to obtain a degreased extract solution (10 mL);

[0099]The extraction solution after the degreasing is loaded (sample loading speed is 1mL / min) in advance with 3mL methanol, the PCX solid-phase extraction column activated by 3mL ultrapure water, then with 3mL0.1mol / L hydrochloric acid and 3mL methanol Rinse the small column, drain it after rinsing, and elute with 3mL me...

Embodiment 3

[0110] After placing 1 g of sesame oil in a polypropylene centrifuge tube, add 80 μL of internal standard working solution (4,7,8-TriMeIQx solution, the concentration is 500 ng / mL) and 10 mL of 1% ammonia acetonitrile solution in sequence, vortex for 5 min, sonicate for 10 min, 3500r Centrifuge 5min under the rotating speed of / min, obtain the first supernatant and residue;

[0111] The residue was repeatedly extracted once according to the above steps to obtain a second supernatant;

[0112] After mixing the first supernatant and the second supernatant, add 10 mL of saturated n-hexane solution of acetonitrile for degreasing to obtain a degreased extract solution (10 mL);

[0113] The extraction solution after the degreasing is loaded (sample loading speed is 1mL / min) in advance with 3mL methanol, the PCX solid-phase extraction column activated by 3mL ultrapure water, then with 3mL0.1mol / L hydrochloric acid and 3mL methanol Rinse the small column, drain it after rinsing, and ...

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Abstract

The present invention relates to the technical field of detection of heterocyclic amines, in particular to a method for detecting heterocyclic amines in the grease. The method for detecting heterocyclic amines in the grease provided by the present invention comprises the following steps: preprocessing a to-be-detected grease sample to obtain the preprocessed grease; and sequentially using the ultra-high performance liquid chromatography and the triple quadrupole mass spectrometry to perform detection on the preprocessed grease, wherein the preprocessing comprises solvent extraction, degreasingprocessing, and solid phase extraction, and the solvent extraction is organic solvent extraction or alkaline organic solvent extraction. The method provided by the present invention has a simple operation and short time-consumption, and can be used to effectively detect heterocyclic amines in the grease.

Description

technical field [0001] The invention relates to the technical field of detection of heterocyclic amines, in particular to a method for detecting heterocyclic amines in oil. Background technique [0002] With the rapid development of my country's economy and the continuous improvement of people's living standards, food safety issues have become the focus of attention. As an important source of daily energy and essential fatty acids, oils and fats have drawn more attention to their safety. However, during the processing of oil from oilseeds to finished oils, it is easy to be polluted by heterocyclic amine compounds, which are carcinogenic and mutagenic (Sanz-Alaejos, M., & Afonso, A.M.(2011).Factors that affect the content of heterocyclic aromatic amines in foods. Comprehensive Reviews in Food Science and Food Safety, 10, 52-108. De Meester, C. (1995). Genotoxic potential of beta-carbolines: A review. Mutation Research, 339, 139-153.) . The existence of heterocyclic amines ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 汪学德张晨霞马宇翔席俊赵天培秦召
Owner HENAN UNIVERSITY OF TECHNOLOGY
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