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Lactobacillus casei SYF-08 capable of adsorbing heavy metal lead and application of lactobacillus casei SYF-08

A technology of SYF-08, Lactobacillus casei, applied in Lactobacillus, application, bacteria used in food preparation, etc., can solve the problem of no reported separation, reduce lead absorption and chronic lead poisoning, stabilize content, reduce recognition effects of functional impairment

Pending Publication Date: 2019-12-31
GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strains are not isolated from plants, and there is no report on the isolation of lead-absorbing strains from infants and young children.

Method used

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  • Lactobacillus casei SYF-08 capable of adsorbing heavy metal lead and application of lactobacillus casei SYF-08
  • Lactobacillus casei SYF-08 capable of adsorbing heavy metal lead and application of lactobacillus casei SYF-08
  • Lactobacillus casei SYF-08 capable of adsorbing heavy metal lead and application of lactobacillus casei SYF-08

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Isolation of Lactobacillus casei Strains

[0030] Take an appropriate amount of fresh feces of healthy newborns who are exclusively breastfed, and add 5mg / L lead solution to dilute according to the solid-liquid ratio of 1:1000 (g / ml). Take 5 μl sample and inoculate it in the modified MRS medium (central diffusion method) (drawing a plate radially from the center to the edge). 200 μl of lead solution with a concentration of 10,000 mg / L was added dropwise to the center of the plate, and incubated anaerobically at a constant temperature of 37°C for 48 hours. Pick a single colony close to the center of the agar plate and inoculate it into the MRS liquid medium for enrichment culture.

[0031]After culturing for 24 hours, dilute with sterile PBS to prepare a bacterial suspension with a wavelength of 600nm and OD=1; centrifuge (8000rpm, 5min), remove the supernatant, add 1ml of 10ppm lead acetate solution respectively; mix well, and let stand at 37°C for 1h Centrifugal (120...

Embodiment 2

[0065] The test of embodiment 2 Lactobacillus casei SYF-08 probiotic properties

[0066] 1. Acid and bile salt resistance

[0067] Prepare artificial gastric juice with pH value of 2.0, 3.0, 4.0 respectively. Take Lactobacillus casei SYF-08, dilute to 10 with sterile PBS 9 CFU / ml bacterial suspension. Pipette 10 μl of the bacterial suspension into 990 μl of artificial gastric juice with different pH values, incubate anaerobically at 37°C for 1-3 hours, and use the dilution drop plate method to determine the number of viable bacteria at the initial stage and after cultivation.

[0068] Bile resistance test: the bile salt comprehensive test (0.3% bile salt concentration) was carried out on the strains that passed the acid resistance screening. The counted strains were placed in pH 3.0 phosphate buffer, cultured anaerobically at 37°C for 2 hours, inoculated 10 μL in 990 μL solution with a bile salt concentration of 0.3%, cultured anaerobically at 37°C for 8 hours, and determin...

Embodiment 3

[0086] 1. Establishment of chronic lead poisoning mouse model

[0087] 32 6-week-old male mice were fed adaptively for 1 week (lighting cycle 12h, temperature 22-26°C, humidity 40%-60%), were randomly divided into 4 groups, 8 in each group, and processed according to Table 4:

[0088] Table 4 Grouping and treatment of animal experiments

[0089] group Approach PBS group Drinking sterile deionized water and gavage with sterile PBS 100 μl / d for 5 consecutive weeks SYF-08 group Drinking sterile deionized water, SYF-08 10 9 / 100μl / d orally for 5 weeks

Pb group Drinking 100mg / L sterile lead water and gavage with sterile PBS 100μl / d for 5 consecutive weeks SYF-08 group + Pb group Drinking concentration is 100mg / L sterile lead water, SYF-08 10 9 / 100μl / d gavage, continuous gavage for 5 weeks

[0090] 2. Morris water maze test to evaluate the cognitive function of mice

[0091] After gavage, the Morris water maze experiment was carr...

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Abstract

The invention discloses lactobacillus casei SYF-08 capable of adsorbing heavy metal lead and an application of the lactobacillus casei SYF-08. The lactobacillus casei SYF-08 is separated from excrement and urine of healthy newborns, has the capacity of bearing lead ions and highly adsorbing the lead ions, can be stably colonized in intestinal tracts and can adsorb the lead ions entering the intestinal tracts. The invention also discloses an application of bacterial liquid of the strain SYF-08 to preparation of food additives, foods or medicines having the efficacy of preventing and / or assisting in treating chronic lead poisoning.

Description

technical field [0001] The invention belongs to the field of micro-ecological living bacteria preparations, in particular to Lactobacillus casei SYF-08 which absorbs heavy metal lead and its application. Background technique [0002] Lead is often used in industrial production due to its stable properties and special physical-chemical structure. But at the same time, it also brought negative impacts. Automobile exhaust, industrial waste, and sewage all contain a lot of heavy metals. Research data shows that lead-containing particles are detected in atmospheric particulate matter (Particulate Matter, PM 2.5) with a particle size below 2.5 μm, and lead-containing particles are positively correlated with the content of PM2.5. In addition, items such as cosmetics and lead paints can expose the human body to high levels of lead. Long-term exposure to high lead environment will seriously affect human health, especially for children. For example, long-term low-dose exposure to l...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L5/20A61K35/747A61P39/02A61P31/04C12R1/245
CPCA23L33/135A23L5/20A61K35/747A61P39/02A61P31/04C12R2001/245C12N1/205A23V2400/125Y02A50/30
Inventor 范宏英吴清平吴军林陈振辉
Owner GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD
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