Injectable collagen composition and preparation method
A composition and collagen technology, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of limited material sources, low biosafety, short degradation cycle, etc., and achieve biocompatibility The effect of good performance, high safety, excellent mechanical properties and degradation properties
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[0040] The preparation method of the above-mentioned injectable collagen composition of one embodiment, refer to figure 1 , the method includes:
[0041] S1: The biological tissue is sterilized to obtain the sterilized biological tissue.
[0042] Wherein, the biological tissue includes at least one of fascia tissue, skin tissue, small intestinal submucosa tissue, amniotic membrane tissue, tendon tissue, blood vessel tissue and nerve tissue. The above-mentioned biological tissue has a relatively high collagen content and is a degradable material that is easily obtained, and the injectable collagen composition prepared by using the above-mentioned biological tissue has good biocompatibility.
[0043] The disinfection treatment may be any conventional method. In one embodiment, the disinfection treatment includes: treating the biological tissue with ethanol solution, the concentration of the ethanol solution may be 75% by weight, the treatment time may be 1h-3h, and finally cle...
Embodiment 1
[0066] (1) Soak the fascia tissue in 75% by weight alcohol solution for 2 hours for disinfection, then wash it with pure water several times, and cut it into 2 mm square sheet tissue.
[0067] (2) The above sheet tissue was treated with hypotonic solution (10mM Tris-HCl, 50mM EDTA) for 11h, and then with hypertonic solution (50mM Tris-HCl, 1M NaCl, 10mM EDTA) for 11h.
[0068] (3) The material obtained in step (2) was washed 4 times with 10°C pure water, and then the first crushing treatment was carried out in a tissue grinder (manufactured by Waring Company, model 8011ES), and then the crushed material was washed with 10 0.4% by weight of sodium hydroxide solution soaked at 4°C for 6 hours, freeze-thawed at -60°C for 2 hours, thawed naturally after taking out, repeated freeze-thaw 3 times, and then washed with pure water to obtain the first impurity-removing treatment. materials.
[0069] (4) the material after the first impurity removal treatment that step (3) obtains is cl...
Embodiment 2
[0075] The preparation steps of the injectable collagen composition in this example are roughly the same as those in Example 1, the difference is that in step (7), three types of collagen compositions with particle sizes ranging from 30 μm to <120 μm, 120 μm to <210 μm, and 210 μm to <300 μm were obtained by grinding and sieving The concentration of the decellularized collagen particles in step (8) is 10 mg / mL, 50 mg / mL, and 90 mg / mL in sequence.
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