UV optical adhesive and uses as pathological section sealing adhesive

[0030] Embodiment 1, the preparation of UV optical glue of the present invention

[0031] In the UV optical adhesive of the present invention, polyethylene glycol methyl ether acrylate is used as a prepolymer, vinyl acetate is used as a reactive diluent, and 2-hydroxyl-2-methyl-1-phenyl-1-acetone is used as photoinitiator.

[0032] The specific preparation method is as follows: according to the formula shown in Table 1, measure each raw material respectively, mix them evenly, and obtain the product.

[0033] Table 1. UV Optical Adhesive Formulation (mL)

[0034]

[0035]

[0036] The beneficial effects of the present invention are demonstrated through experimental examples below.

[0037] Experimental example 1. Drying time test of UV optical glue

[0038] 1. Experimental method

[0039] (1) Test materials: the UV optical adhesive prepared in Example 1; neutral resin mounting agent (Thermo ClearVue mounting agent; product number 4212; batch number 406379);

[0040] (2) Slicing production:

[0041] a. Grouping: Take 10 HE-stained pathological slides that have been stained, divide them into 2 groups (neutral resin group, UV optical glue group), and mark them;

[0042] b. Mounting: According to the section grouping, drop the same volume of neutral resin mounting agent and the UV optical glue prepared in Example 1 on the slide, then cover with a cover glass, and place carefully after sealing drying plate;

[0043] (3) Drying: After the neutral resin group is sealed, put it in a ventilated place at room temperature to dry and cure naturally. After the UV optical glue group is sealed, put it into an ultraviolet light irradiation device (DEYIWISE UV glue curing lamp; model JD818; power 36W) to cure .

[0044] (4) Record the drying time of each slice.

[0045] Drying standard: place the slices overlapping for 3 hours, and the front and back slices are not stuck together.

[0046] 2. Experimental results

[0047] The results are shown in Table 2. It can be seen that the prepared UV optical adhesive of the present invention dries rapidly after being irradiated in an ultraviolet irradiation box, and the drying time is 10 to 30 seconds, wherein sample 42 (prepolymer: reactive diluent: photoinitiator=9: 0.8:0.2) had the fastest drying speed, and the drying time was 10s; while the average drying time of the neutral resin group after mounting was 96 hours. Therefore, the drying speed of the prepared UV optical adhesive of the present invention is significantly faster than that of the control neutral resin mounting agent.

[0048] Table 2. Drying time of each slice

[0049]

[0050] Experimental example 2. Test of the sealing effect of UV optical glue

[0051] 1. Experimental method

[0052] (1) Use the UV optical glue (sample 42) and neutral resin mounting agent prepared in Example 1 to mount the following stained paraffin pathological sections: HE staining, Masson staining, glycogen staining, formazan Aniline blue staining, Sirius red staining, and immunohistochemical staining, and then collect images to observe the effect of sectioning after mounting.

[0053] (2) UV optical glue (sample 42) and neutral resin mounting agent prepared in Example 1 are used to mount the following frozen pathological sections that have been stained: HE staining, then collect images, observe after mounting slice effect.

[0054](3) After sealing the HE-stained pathological sections with the UV optical glue (sample 42) prepared in Example 1, the images were collected; stored at room temperature under dry and ventilated conditions for 2 years, and the images were collected again.

[0055] 2. Experimental results

[0056] The result is as figure 1 and figure 2 As shown, it can be seen that no matter whether it is a paraffin section or a frozen section, after sealing with the UV optical glue and neutral resin mounting agent prepared in Example 1, the color of each section is clear and there is no obvious difference.

[0057] from image 3 It can be seen that the pathological slices can be stored for a long time after being sealed with the UV optical glue prepared by the present invention. After 2 years of storage, the UV optical glue can still keep the color of the pathological slices clear without affecting the judgment of pathologists on the pathological slices.

[0058] In summary, the present invention utilizes low-toxic raw materials, with polyethylene glycol methyl ether acrylate as a prepolymer, vinyl acetate as a reactive diluent, and 2-hydroxy-2-methyl-1-phenyl-1- Acetone was used as a photoinitiator to prepare a liquid UV optical glue that can be applied to fast mounting of pathological sections. Experimental results show that the UV optical glue prepared by the present invention can dry quickly within 10-30 seconds under ultraviolet light irradiation, while the drying time of traditional neutral resin mounting glue needs 96 hours. The UV optical glue of the present invention can significantly increase the drying speed of pathological slices, and greatly improve the efficiency of making pathological slices. Furthermore, the dried UV optical glue of the present invention is resistant to high temperature and high pressure, long-term storage, does not change color, and can still keep the color of pathological sections clear after storage for more than two years, and has a very good application prospect in the production of pathological sections.