Chromium-contaminated land remediation method

A remediation method and technology for chromium pollution, applied in the field of bioremediation of chromium-contaminated soil, can solve the problems of high mutagenicity and toxicity, high cost, mutagenicity and carcinogenicity are harmful to the soil environment, etc., and achieve a long period of stability , low cost, and the effect of improving soil physical and chemical properties

Inactive Publication Date: 2020-01-10
WUHAN UNIV OF SCI & TECH
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  • Claims
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Problems solved by technology

On the contrary, Cr 6+ has a ratio of Cr 3+ Higher mutagenicity and toxicity, and Cr 6+ More harmful to the soil environment due to its mutagenic and carcinogenic properties
The existing remediation technologies for heavy metal contaminated soil mainly include physical remediation, chemical remed

Method used

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  • Chromium-contaminated land remediation method
  • Chromium-contaminated land remediation method
  • Chromium-contaminated land remediation method

Examples

Experimental program
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preparation example Construction

[0026] Preparation of chromium-contaminated soil: take 200g of uncontaminated dry soil, weigh 400g of potassium dichromate solution with a concentration of 500mg / L, spread a layer of soil, and spray potassium dichromate solution once, repeating this many times until dichromate Potassium acid potassium solution was added to the weighed soil, and then it was dried, crushed, sieved, and sterilized to obtain Cr 6+ Chromium-contaminated soil with a mass ratio of 500 mg / kg to the soil.

[0027] The TCLP (Toxicity Characteristic Leaching Procedure) method (promulgated by the US Environmental Protection Agency) is used to measure the soil leaching concentration. The soil sample is leached with an acetic acid solution with a pH of 2.88. The soil-water ratio (mass volume ratio) is 1:20. -1 Turn down and shake for 18-20h, use diphenylcarbazide spectrophotometry (GB / T15555.5-1995) to analyze Cr 6+ The concentration was tested, and the blank extract was used as a control during the experi...

Embodiment 1

[0029] Take 33.3g of calcium chloride, 18g of urea and 200mL of distilled water and mix them uniformly to obtain the glue solution; take 20mL of the glue solution and 20mL of bacterial solution A and mix them evenly to get the reaction solution; take 20mL of the reaction solution and add 10g of chromium-contaminated soil to it Place it in a constant temperature incubator at 30°C for 30 days. After 30 days, extract it according to the TCLP method. It is measured that the concentration of hexavalent chromium in the contaminated soil leachate is reduced from 210 mg / L to 2.94 mg / L, and the removal rate of Cr is 98.6 %.

Embodiment 2

[0031] With Example 1, the difference is that the glue solution is obtained after the urea of ​​70.8g calcium nitrate and 18g is dissolved in 200mL of distilled water and mixed uniformly, and the concentration of hexavalent chromium in the polluted soil leachate is reduced from 210mg / L to 4.64mg / L, the removal rate of Cr is 97.79%.

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Abstract

The invention discloses a chromium-contaminated land remediation method. The method comprises the following steps: 1, inoculating a sterilized liquid culture medium with a Bacillus sp. strain, performing culturing to obtain a bacterial stock solution, and adding normal saline into the bacterial stock solution to dilute the bacterial stock solution to make the OD600 value be 0.8-1.5; 2, taking anduniformly mixing an aqueous urea solution and an aqueous calcium chloride solution to obtain an adhesive solution; 3, mixing the bacterial solution prepared in step 1 with the adhesive solution prepared in step 2 to obtain a reaction solution; 4, taking chromium-contaminated soil, removing impurities, and air-drying, crushing and sieving the soil; and 5, uniformly pouring the reaction solution prepared in step 3 into the pretreated soil obtained in step 4, and then carrying out constant-temperature culture for 20-40 d. The restoration remediation method can reduce Cr<6+> into Cr<3+>, and the reduction rate can reach 98%.

Description

technical field [0001] The invention belongs to the field of soil remediation, in particular to a bioremediation method for chromium-contaminated soil. Background technique [0002] Hexavalent chromium (Cr 6+ ) is one of the most dangerous pollutants in soil, highly toxic to all living organisms, especially carcinogenic and genotoxic to humans and animals. The hazard and toxicity of Cr depend on its chemical form. In soil, it mainly exists in two oxidation states: Cr 3+ and Cr 6+ . Cr 3+ Relatively stable and low toxicity. On the contrary, Cr 6+ has a ratio of Cr 3+ Higher mutagenicity and toxicity, and Cr 6+ It is more harmful to the soil environment due to its mutagenic and carcinogenic properties. The existing remediation technologies for heavy metal-contaminated soil mainly include physical remediation, chemical remediation and microbial remediation, and both physical remediation and chemical remediation have the disadvantage of high cost, and because microbial...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 董欢雷学文张彬赵涵洋林胜强刘特
Owner WUHAN UNIV OF SCI & TECH
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