Technique for realizing precisely fixed-point RNA shearing in fish embryos

A technology for precise positioning and zebrafish embryos, applied in the biological field, can solve the problems of easy off-target, long order cycle, high toxicity, etc., and achieve the effect of simple components, low cost, and low toxicity
CN110669795APending Publication Date: 2020-01-10FUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUZHOU UNIV
Publication Date
2020-01-10

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Abstract

The invention provides a technique for realizing precisely fixed-point RNA shearing in fish embryos. A CasRx-mediated RNA editing technique is used, only mRNA of CasRx and specific targeted guidance RNA need to be provided, precisely fixed-point RNA knockdown can be realized in the fish embryos, and the technique has the advantages such as high efficiency, high specificity, convenience and low cost. The problems of faced low efficiency of a current existing siRNA technique and high cost and prone off-target of a Morpholino-technique-mediated gene knockout technique are solved.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a technology for realizing precise fixed-point RNA shearing in fish embryos. Background technique

[0002] With the continuous development and improvement of whole genome sequencing technology and the implementation of large-scale genome annotation projects, biological science research has entered the post-genome era. In the post-genome era, the focus of genome research will shift to gene function, that is, from determining the DNA sequence of genes and explaining all the genetic information of life to the study of biological functions at the molecular level, exploring human health and human health at the molecular level. The mystery of disease (Peltonen and McKusick, 2001). Researchers have begun to make various attempts to apply the results of genome research to various fields of basic scientific research and personalized medicine (Chan and Ginsburg, 2011). However, i...

Claims

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