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Bos grunniens rib small-molecular peptide and extraction method thereof

A small molecule peptide and rib technology, which is applied in the biological field to achieve the effects of low processing cost, high product content and stable product properties

Inactive Publication Date: 2020-01-10
吴伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Collagen polypeptides have become a current research hotspot because of their rich nutritional value and physiological functions. There are many reports on enzymatic hydrolysis of bones at home and abroad, focusing on pig bones, bovine bones, and fish bones. Research on enzymatic hydrolysis of yak bone collagen is scarce see the report

Method used

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  • Bos grunniens rib small-molecular peptide and extraction method thereof

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Comparison scheme
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Embodiment 1

[0054] This embodiment discloses a method for industrial production of yak rib small molecule peptide, which specifically includes the following steps:

[0055] (1) Raw material pretreatment: take a certain amount of fresh yak ribs that pass the inspection and quarantine, and pulverize;

[0056] (2) Cleaning of yak ribs: the yak ribs obtained in step (1) are broken into bones, washed 2-3 times with clear water, until the bones are white or milky white, and clean yak ribs are obtained;

[0057] (3) Extract collagen solution: put the clean yak rib bone fragments obtained in step (2) into the reactor, add 2-5 times the quality of water, and cook at high temperature and high pressure for 4-6 hours;

[0058] (4) Standing to remove oil: the extract obtained in step (3) is left to stand for 2-3 hours at room temperature in a reserved tank, and after the oil layer and the material liquid layer are separated, the oil layer is separated;

[0059] (5) Step-by-step enzymolysis: add 0.1%-...

Embodiment 2

[0073] This embodiment discloses a yak rib small molecule peptide powder, which is prepared by the method disclosed in Example 1.

[0074] The yak rib small molecular peptide powder has the advantages of fast absorption and high utilization rate, and has good solubility and stability, relatively concentrated molecular weight, and the molecular weight is less than 1000Da, the product is stable, easily absorbed by the human body, and can be widely used in the field of medicine or food. Specifically, the yak rib small molecule peptide can be used to prepare drugs for assisting blood pressure lowering, anti-oxidation, prevention and treatment of osteoarthritis and osteoporosis, and can also be used to prepare health food or nutritional products.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a bos grunniens rib small-molecular peptide and an extraction method thereof. The extraction method includes the following steps: S1, by using bos grunniens ribs as raw materials, performing pretreatment on the raw materials; S2, extracting a collagen solution; S3, allowing the collagen solution to stand for degreasing; S4, performing an enzymatic hydrolysis reaction; and S5, performing purification to obtain the small-molecular peptide solution. According to the extraction method disclosed by the invention, the biological enzymolysis technology is used, the bos grunniens ribs are converted into the water-soluble active ingredient small-molecular peptide through the biological enzymatic hydrolysis, the small-molecular peptide obtained after the enzymatic hydrolysis does not need to be separated and has a relatively-concentrated molecular weight, the molecular weight is < 1000 Da, and the product has stable quality and is easily absorbed by human bodies; the operation process has the simple and convenient steps, the various links are easy to control, and the processing cost is low; and in the production process, high-quality bos grunniens tallow without addition can be obtained, the content of polyunsaturated fatty acids of the bos grunniens tallow is higher, and the polyunsaturated fatty acidshave a plurality of physiological functions, and can be fully utilized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a yak rib small molecule peptide and an extraction method thereof. Background technique [0002] Yak (Bos grunniens) is a rare animal species, mainly distributed in the Qinghai-Tibet Plateau, Tianshan Mountains, Altai Mountains, Sayan Mountains to the southeast of Lake Baikal. It is a famous landscape cattle species on the "roof of the world". for yak. There are more than 15 million yaks in the world. Among them, China has more than 14 million yaks, accounting for more than 95% of the total number of yaks in the world. It is the country with the largest number and variety of yaks in the world. [0003] Yak is distributed in the pollution-free Qinghai-Tibet Plateau region. It is an authentic green product and is increasingly favored by the market. Yak meat will become more and more popular with consumers. More importantly, its blood, meat, tendons, and bones are precious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14C07K1/34A61K38/39A61P9/12A61P39/06A61P19/02A61P19/10A61P29/00A23J1/10A23J3/04A23J3/34A23L33/18
CPCA23J1/10A23J3/04A23J3/342A23L33/18A23V2002/00A61K38/00A61P9/12A61P19/02A61P19/10A61P29/00A61P39/06C07K14/78C12P21/06A23V2200/30A23V2200/326A23V2200/306A23V2250/55A23V2300/14A23V2300/38Y02P20/10Y02P20/50
Inventor 不公告发明人
Owner 吴伟