Method for analyzing stable isotopes of granular organic carbon and nitrogen in seawater by using EA-IRMS

A granular organic carbon and isotope technology, which is applied in the direction of chemical analysis, analytical materials, and test sample preparation by means of combustion, can solve the problems of inability to separate granular and dissolved states, achieve reuse, ensure reliability, The effect of reducing work intensity and requirements

Active Publication Date: 2020-01-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, on the basis of the national standard, we have improved the element content analysis method of particulate organic carbon and particulate nitrogen, that is, the method of blank value calibration is used in the element analysis. However, due to the particularity of isotope analysis, the instrument completely burns the sample All will be sent into the detection magnetic field, the particle state and the dissolved state cannot be separated, and the obtained isotope ratio cannot be used for elemental analysis to deduct the influence of dissolved organic carbon by means of integral calculation. The stable isotope detection obviously cannot be corrected by blank value

Method used

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  • Method for analyzing stable isotopes of granular organic carbon and nitrogen in seawater by using EA-IRMS
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  • Method for analyzing stable isotopes of granular organic carbon and nitrogen in seawater by using EA-IRMS

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Effect test

Embodiment 1

[0024] In this embodiment, a large number of exploratory experiments on the influence of temperature on the data results and the bursting of the combustion tube were carried out, and the temperature setting of the technical solution of the present invention was finally obtained to be 700°C. During the experiment, it was found that when the temperature is lower than 700 °C, incomplete combustion will occur, and the standard curve will have poor linearity. After the subsequent inserted standard is brought into the standard curve, the correction factor will also appear In the case of large cases, we believe that the working temperature of the instrument should not be lower than 700°C. It should be noted that at 700°C, it is best to set the oxygen addition time of the instrument for 10 seconds to ensure complete combustion. When the temperature is higher than 700°C, it is found through statistics that the probability of cracking or bursting of the combustion tube at 850°C is the sa...

Embodiment 2

[0026]In this embodiment, a groping experiment was carried out on the influence of the amount of oxygen added on the data results, and finally the temperature setting of the technical solution of the present invention was set to 700°C. When this embodiment tests the oxygen addition amount of the instrument, when the conventional oxygen addition amount of the instrument is 70s, at a temperature of 700 degrees, the conversion rates of standard C and N are respectively 99.1% and 99.2%; after changing the oxygen addition amount to 80s, At 700°C, the conversion rates of standard C and N are 100.2% and 99.6%, so after the temperature is set to 700°C, it is best to increase the amount of oxygen added for 10 seconds to ensure that the sample is fully combusted.

Embodiment 3

[0028] 1. Materials and methods

[0029] 1.1 Materials

[0030] 1.1.1 Membrane

[0031] On June 2, 2019, a φ25mm GF / F glass fiber filter membrane was prepared in the laboratory. After burning in a muffle furnace at 450°C for 5 hours, it was taken out and placed in an electronic moisture-proof box to cool down for later use.

[0032] 1.1.2 Water sample

[0033] On June 4, 2019, surface and bottom seawater samples were collected in the breeding sea area of ​​Dongchu Island Aquatic Products Co., Ltd., Rongcheng City, Shandong Province, and were immediately filtered after being transported back to the laboratory.

[0034] 1.2 Method

[0035] 1.2.1 Collection of samples

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Abstract

The invention relates to a method for analyzing the stable isotopes of granular organic carbon and nitrogen in seawater by using EA-IRMS, and belongs to the field of water quality detection. The method starts from the whole process of sample collection, storage, treatment and detection; instrument settings and parameters are changed; after a collected sample is filtered, the filtered sample is filtered with 100ml of distilled water; the oxygen adding amount of an element analyzer is set to be 80 s; the temperature of a combustion tube is set to be 700 DEG C; and the filament current of an isotope mass spectrometer is set to be 300-400 microamperes. With the method adopted, detection cost is reduced by 30%, data accuracy is improved, and working efficiency is improved.

Description

technical field [0001] The invention belongs to the field of water quality detection, in particular to a method for analyzing particulate organic carbon and nitrogen stable isotopes in seawater by using EA-IRMS. It utilizes the high-efficiency, fast and accurate characteristics of the elemental analyzer and the isotope mass spectrometer, and combines the method of the invention to achieve the purposes of saving consumables, improving data accuracy and prolonging the service life of the instrument. Background technique [0002] Advanced scientific research facilities and instruments are the key to breakthroughs in scientific and technological innovation, which promote and support the development of scientific research. At present, the country is vigorously developing the marine blue economy, and emerging fields such as carbon sink fishery and marine ranching are gradually emerging. Green, healthy, and efficient have become key words in the development of aquaculture, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N31/12G01N1/34G01N1/44
CPCG01N27/62G01N31/12G01N1/34G01N1/44G01N1/4077G01N2001/4088G01N1/42G01N33/188
Inventor 刘毅张继红吴文广孙科
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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