Application of graphene oxide in improving soil consolidation strength, composition and application method of composition

A technology of consolidation strength and composition, applied in the field of microbially induced calcium carbonate soil improvement, which can solve the problems of incomplete consumption of calcium ions, waste of resources, and reduction of calcium carbonate production

Inactive Publication Date: 2020-01-14
CHINA UNIV OF GEOSCIENCES (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, in the use of the existing microorganism-induced calcium carbonate precipitation and consolidation soil, the calcium ions cannot be completely consumed due to the insufficient hydrolysis of urea, resulting in a waste of resources
Reduced calcium carbonate production due to insufficient reaction of calcium ions
Since the compressive strength of the soil is directly proportional to the production of calcium carbonate, the soil cannot achieve the desired strength

Method used

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  • Application of graphene oxide in improving soil consolidation strength, composition and application method of composition
  • Application of graphene oxide in improving soil consolidation strength, composition and application method of composition
  • Application of graphene oxide in improving soil consolidation strength, composition and application method of composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of Bacillus Pasteurella (ATCC11859) bacteria suspension: Bacillus Pasteurella (ATCC11859) was cultured aerobically at a speed of 120 rpm in a medium at 30°C for 48 hours, and the culture was centrifuged at 5434g at 4°C for 8 Minutes to get the bacterial colony; the above bacterial colony was diluted with deionized water to prepare bacterial suspensions of different concentrations (OD 600 =0.25, 0.5, 0.75 and 1.0).

[0038] Wherein, the above medium is mixed with casein peptone (15g / L), soybean peptone (5g / L), sodium chloride (5g / L) and urea (20g / L) into 1000mL deionized water, the final The pH value is about 7.3, and the medium is prepared by sterilizing at 121° C. for 30 minutes.

[0039] The above-mentioned bacterial suspensions of different concentrations are 0.25 (1.299 × 10 7 cfu / ml), 0.5 (3.34×10 7 cfu / ml), 0.75 (5.804×10 7 cfu / ml) and 1.0 (8.59×10 7 cfu / ml) optical density.

Embodiment 2

[0041] Calcium carbonate precipitation: 0.3mol / L cementing liquid is prepared by mixing 0.6mol / L calcium chloride aqueous solution and 0.6mol / L urea solution. The different concentrations (OD) that embodiment 1 prepares 600 =0.25, 0.5, 0.75 and 1.0) of the bacterial suspension. Calcium carbonate precipitation was carried out at 25 °C in a beaker containing 60 ml of bacterial suspension and 240 ml of cementing liquid (consolidating liquid consisting of 120 mL of calcium chloride and 120 mL of urea solution). When the cementing fluid concentration is 0.3mol / L, at different bacterial concentrations (OD 600 =0.25, 0.5, 0.75 and 1.0), without adding graphene oxide and adding 2 ml of 10 mg / L graphene oxide for precipitation. When the bacterial concentration is equal to OD 600 At 1.0, the same calcium carbonate precipitation was carried out under different concentrations of graphene oxide (5, 10, 15 and 20 mg / L) and cementing fluid (0.3 mol / L).

[0042] Soil consolidation: the ba...

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Abstract

The invention provides an application of graphene oxide in improving soil consolidation strength, a composition and an application method of the composition, and relates to the technical field of microbially induced calcium carbonate soil improvement. According to the application of graphene oxide in improving the soil consolidation strength, the composition and the application method of the composition, the graphene oxide can enable microorganisms to induce the rapid growth of calcium carbonate crystals in calcium carbonate, and the yield of the calcium carbonate is increased; and the size ofthe calcium carbonate crystals can further be changed, the consolidation of soil is facilitated, and the compressive strength of the soil is improved.

Description

technical field [0001] The invention relates to the technical field of microorganism-induced calcium carbonate soil improvement, in particular to the application, composition and use method of graphene oxide in improving soil consolidation strength. Background technique [0002] Microbial induction of calcium carbonate to consolidate soil is the main development trend in the field of soil improvement. Its advantage is that the mechanism of microbially induced calcium carbonate precipitation and soil consolidation is: urease decomposed by microorganisms can hydrolyze urea in solution to produce carbonate ions, carbonate ions and Calcium ions in the solution react to produce calcium carbonate. Calcium carbonate uses bacteria as nuclei to consolidate the soil together, which improves the strength of the soil and is harmless to the environment. development trend. [0003] However, in the use of the existing microorganism-induced calcium carbonate precipitation and consolidation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/42
CPCB09C1/00B09C1/10
Inventor 王贵和唐国旺唐俊王新杰张浩男
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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