Application of graphene oxide in improving soil consolidation strength, composition and application method of composition
A technology of consolidation strength and composition, applied in the field of microbially induced calcium carbonate soil improvement, which can solve the problems of incomplete consumption of calcium ions, waste of resources, and reduction of calcium carbonate production
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Embodiment 1
[0037] Preparation of Bacillus Pasteurella (ATCC11859) bacteria suspension: Bacillus Pasteurella (ATCC11859) was cultured aerobically at a speed of 120 rpm in a medium at 30°C for 48 hours, and the culture was centrifuged at 5434g at 4°C for 8 Minutes to get the bacterial colony; the above bacterial colony was diluted with deionized water to prepare bacterial suspensions of different concentrations (OD 600 =0.25, 0.5, 0.75 and 1.0).
[0038] Wherein, the above medium is mixed with casein peptone (15g / L), soybean peptone (5g / L), sodium chloride (5g / L) and urea (20g / L) into 1000mL deionized water, the final The pH value is about 7.3, and the medium is prepared by sterilizing at 121° C. for 30 minutes.
[0039] The above-mentioned bacterial suspensions of different concentrations are 0.25 (1.299 × 10 7 cfu / ml), 0.5 (3.34×10 7 cfu / ml), 0.75 (5.804×10 7 cfu / ml) and 1.0 (8.59×10 7 cfu / ml) optical density.
Embodiment 2
[0041] Calcium carbonate precipitation: 0.3mol / L cementing liquid is prepared by mixing 0.6mol / L calcium chloride aqueous solution and 0.6mol / L urea solution. The different concentrations (OD) that embodiment 1 prepares 600 =0.25, 0.5, 0.75 and 1.0) of the bacterial suspension. Calcium carbonate precipitation was carried out at 25 °C in a beaker containing 60 ml of bacterial suspension and 240 ml of cementing liquid (consolidating liquid consisting of 120 mL of calcium chloride and 120 mL of urea solution). When the cementing fluid concentration is 0.3mol / L, at different bacterial concentrations (OD 600 =0.25, 0.5, 0.75 and 1.0), without adding graphene oxide and adding 2 ml of 10 mg / L graphene oxide for precipitation. When the bacterial concentration is equal to OD 600 At 1.0, the same calcium carbonate precipitation was carried out under different concentrations of graphene oxide (5, 10, 15 and 20 mg / L) and cementing fluid (0.3 mol / L).
[0042] Soil consolidation: the ba...
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