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Application of Akkermansia Muciniphila in preparing anti-depression drug or health-care product

A technology of health care products and medicines, applied in the field of probiotics, to achieve good control effects, safe use, and good curative effect

Active Publication Date: 2020-01-17
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on its use in the prevention and treatment of depression

Method used

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  • Application of Akkermansia Muciniphila in preparing anti-depression drug or health-care product
  • Application of Akkermansia Muciniphila in preparing anti-depression drug or health-care product
  • Application of Akkermansia Muciniphila in preparing anti-depression drug or health-care product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. The preparation method of Akkermansia Muciniphila bacterial liquid:

[0033] Take 1ml of Akkermansia Muciniphila bacteria solution, add it into 10ml of brain heart extract broth, incubate at 37°C for 72 hours under anaerobic environment, centrifuge the bacteria solution (6000rpm) for 20 minutes, separate the supernatant, and dissolve it with sterile PBS solution. The bacterial solution was adjusted to OD600=1 to obtain the Akkermansia Muciniphila bacterial solution (abbreviated as AKK bacterial solution).

[0034] 2. Preparation method of pasteurized AKK bacteria (dead bacteria)

[0035] Place the AKK bacterial solution obtained in step 1 in a water bath and heat to 62-65°C for 30 minutes.

[0036] 3. Preparation method of Amuc_1100

[0037] By amplifying the gene encoding the intracellular part of 1100, the resulting PCR product was cloned into the pET32aTEV vector by restriction enzyme ligation to construct the expression plasmid of Amuc_1100 fused to the His tag...

Embodiment 2

[0074] Example 2 investigates the therapeutic effect of AKK bacteria on depression-complicated enteritis model mice

[0075] 1. Construction of chronic restraint stress depression mouse model (Chronic restraint stress, CRS) The specific modeling method of CRS is as follows: take male C57BL / 6 mice, aged 6-8 weeks. After one week of adaptive feeding, the mice were placed in a 0.5mm diameter, 50ML centrifuge tube for 3 hours every day for 30 days. Behavioral test results were recorded on day 30. During the CRS modeling period, the mice in the other groups were restricted from feeding and watering; at the end of the experiment, behavioral tests were performed on all mice, including: open field test, tail suspension test and forced swimming test. After the behavioral test showed that the depression model was successfully established, the mice were sacrificed immediately and the contents of the cecum were removed. The caecal contents of 3 mice were randomly mixed, dissolved in ste...

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Abstract

The invention discloses application of Akkermansia Muciniphila in preparing a drug or health-care product for preventing and treating depression. In the application, in vivo pharmacodynamic research is performed on the effect of the Akkermansia Muciniphila in incidence of the depression and depression resistance; a result shows that abundance of the Akkermansia Muciniphila in an intestinal tract of a patient with the depression is obviously lowered; and a result of an animal experiment shows that abundance of the Akkermansia Muciniphila in an intestinal tract of a mouse with the depression isremarkably lowered. In the application disclosed by the invention, the Akkermansia Muciniphila is transplanted into a model of the mouse with the depression; and tests show that through Akkermansia Muciniphila transplantation, a movement distance of the mouse can be increased, the immobility times of a tail suspension test and a forced swimming test can be shortened, the depression state of the mouse is remarkably relieved, and the Akkermansia Muciniphila shows very good anti-depression curative effect without side effect.

Description

technical field [0001] The invention relates to a probiotic, in particular to the new use of Akkermansia Muciniphila in antidepressant drugs or health products, and belongs to the technical field of medicine. Background technique [0002] Depression is a mental illness characterized by persistent and significant low mood. It is often accompanied by low mood, low self-esteem and depression, and even suicide attempts or behaviors, and most patients have a tendency to relapse. Therefore, depression is a disease with a high disabling rate. According to the World Health Organization (WHO), 15 out of every 100 people in the general population suffer from depression. The cost of treating depression accounts for 12.3% of the total medical expenditure every year. In China, it is estimated that there are 20-50 million depression patients, and the prevalence is increasing year by year. Currently, there is no safe and effective antidepressant biological agent in the market. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P25/24A61P1/00A61P35/00A61P3/10A61P3/04A61P25/08A23L33/135
CPCA23V2002/00A61K35/741A23L33/135A61P1/00A61P3/04A61P3/10A61P25/08A61P25/24A61P35/00A23V2200/322A23V2200/32A23V2200/308
Inventor 陈玉根陈拓袁晓敏王琼施国平
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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