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Bacillus amyloliquefaciens strain for preventing and treating potato black scurf, microbial agent and their application

A technology for potato black mole disease and amylolytic spores, which is applied in application, fungicides, pest control, etc., can solve problems affecting potato yield and quality, aggravation of soil-borne diseases, and economic losses in the potato industry, and is suitable for industrial production , Promote crop growth, save manpower and use cost

Active Publication Date: 2020-01-17
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to continuous cropping of potatoes, soil-borne diseases have been increasing year by year, which directly affects the yield and quality of potatoes, and has brought great economic losses to the potato industry.

Method used

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  • Bacillus amyloliquefaciens strain for preventing and treating potato black scurf, microbial agent and their application
  • Bacillus amyloliquefaciens strain for preventing and treating potato black scurf, microbial agent and their application
  • Bacillus amyloliquefaciens strain for preventing and treating potato black scurf, microbial agent and their application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The present embodiment provides a kind of preparation method of the microbial inoculant of effectively preventing and treating potato black mole, and concrete steps comprise:

[0038] (1) Activate the Bacillus amyloliquefaciens HMB33604 strain stored at -80°C on LB plate medium (tryptone 8g, yeast extract 4g, sodium chloride 4g, agar powder 12g, water 1000mL) at 28°C for 20h , pick a single colony on LB slant medium (tryptone 8g, yeast extract 4g, sodium chloride 4g, agar powder 12g, water 1000mL), and cultivate it at 25°C for 12h to obtain the cultured strain;

[0039] (2) Inoculate the cultured strain into LB liquid medium (8 g of tryptone, 4 g of yeast extract, 4 g of sodium chloride, and 1000 mL of water), and culture with shaking at 28° C. for 20 h at a shaking speed of 160 r / min. get the seed solution;

[0040] (3) Insert the seed liquid into a fermentation medium (12 g of glucose, 13 g of soybean powder, 4 g of sodium chloride, 0.3 g of manganese sulfate, and 10...

Embodiment 2

[0042] The present embodiment provides a kind of preparation method of the microbial inoculant of effectively preventing and treating potato black mole, and concrete steps comprise:

[0043] (1) Activate the Bacillus amyloliquefaciens HMB33604 strain stored at -80°C on LB plate medium (tryptone 12g, yeast extract 6g, sodium chloride 6g, agar powder 18g, water 1000mL) at 34°C for 24h , pick a single colony on LB slant medium (tryptone 12g, yeast extract 6g, sodium chloride 6g, agar powder 18g, water 1000mL), and cultivate it at 35°C for 18h to obtain the cultured strain;

[0044](2) Inoculate the cultured strain into LB liquid medium (12 g of tryptone, 6 g of yeast extract, 6 g of sodium chloride, and 1000 mL of water), and culture with shaking at 32° C. for 28 h at a shaking speed of 200 r / min. get the seed solution;

[0045] (3) Insert the seed liquid into a fermentation medium (18 g of glucose, 17 g of soybean powder, 6 g of sodium chloride, 0.5 g of manganese sulfate, and ...

Embodiment 3

[0047] The present embodiment provides a kind of preparation method of the microbial inoculant of effectively preventing and treating potato black mole, and concrete steps comprise:

[0048] (1) Activate the Bacillus amyloliquefaciens HMB33604 strain stored at -80°C on LB plate medium (tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) at 31°C for 22h , pick a single colony on LB slant medium (tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), and cultivate it at 30°C for 15h to obtain the cultured strain;

[0049] (2) Inoculate the cultured strain into LB liquid medium (10 g of tryptone, 5 g of yeast extract, 5 g of sodium chloride, and 1000 mL of water), and culture with shaking at 30° C. for 24 hours at a shaking speed of 180 r / min. get the seed solution;

[0050] (3) Insert the seed liquid into a fermentation medium (15 g of glucose, 15 g of soybean powder, 5 g of sodium chloride, 0.4 g of manganese sulfate,...

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Abstract

The invention relates to a Bacillus amyloliquefaciens strain for preventing and treating potato black scurf, a microbial agent and their application, and particularly discloses a Bacillus amyloliquefaciens strain HMB33604, and application of the HMB33604 strain in preventing and treating the potato black scurf. The strain is already collected in the China General Microbiological Culture CollectionCenter under CGMCC No. 18469. A fermentation liquid and fermentation supernatant of the Bacillus amyloliquefaciens strain HMB33604 provided by the invention have a strong inhibition effect upon the pathogenic bacterium Rhizoctonia solani of potato black scurf; in addition, the microbial agent disclosed by the invention is safe to people and livestock, does not cause the problems of environmentalpollution and the like, and simple to use, and a preparation method of the microbial agent is simple and low in cost.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and in particular relates to a Bacillus amyloliquefaciens bacterial strain for effectively preventing and treating potato black mole, as well as its bacterial agent and application. Background technique [0002] Potato is an important food crop in my country, which is of great significance to ensure food security. my country has listed potatoes as the fourth staple food after rice, wheat and corn. my country's potato planting area and total output both rank first in the world, but the yield per unit area is lower than the world average due to bottleneck factors such as diseases and insect pests. In recent years, soil-borne diseases have been increasing year by year due to continuous cropping of potatoes, which directly affects the yield and quality of potatoes, and has brought great economic losses to the potato industry. [0003] Potato black mole disease is also known as rhizoct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCC12N1/20C12R2001/07C12N1/205
Inventor 郭庆港马平
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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