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Biocontrol streptomycete

A technology for biological control of Streptomyces and aerial mycelium is applied in the field of microorganisms to achieve the effects of good antibacterial effect, great biological control potential and stable field control effect.

Active Publication Date: 2020-01-17
JILIN CITY ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on October 10, 2019

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 1.1.1 Strain screening

[0070] Select Gaoshi No. 1 synthetic medium, use multi-concentration gradient dilution coating method to isolate and screen strains, observe and select strains with different colony shapes every day, and transfer them to Gaoshi No. 1 slant for culture in time, and use dilution separation method to re-purify After 3-5 times, store the numbers in a 4°C refrigerator for later use.

[0071] 1.1.2 Determination of antibacterial activity of actinomycetes

[0072] Transfer all the isolated bacterial strains to Gaoshi No. 1 medium for culture, and after each bacterial strain produces a sufficient amount of spores, use the blueberry canker sore bacteria as the target bacteria, and measure the activity of the actinomycetes isolated in 1.1.1 by the plate confrontation culture method. For antibacterial activity, prepare a 7mm blueberry canker bacteria cake and inoculate it in the center of a PDA plate (90mm in diameter), pick up the actinomycetes cultured ...

Embodiment 2

[0104] Embodiment 2 result and analysis

[0105] 2.1 Initial screening of strains

[0106] After purification, 84 strains of purified bacterial strains with consistent shape, size and color were obtained, and the plate confrontation test results showed that there were 12 bacterial strains (as shown in Table 1) with a bacteriostatic zone width greater than 10mm, and those with a bacteriostatic zone greater than 15mm had 8 bacterial strains, wherein the bacterial strain CX3 has good antibacterial effect ( figure 1 ), and the width of the inhibition zone was 16.16mm, so the strain CX3 (the strain was screened from the samples collected from the Ma'anshan spruce plantation at the Chixi Protection Station of Changbai Mountain Reserve) was selected as the strain for the next test.

[0107] Table 1 Antibacterial activity of Streptomyces living organisms against blueberry canker bacterium

[0108]

[0109] The data in the table are mean ± standard deviation.

[0110] 2.2 Determi...

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Abstract

The invention relates to biocontrol streptomycete. The preservation number of the strain of the biocontrol streptomycete is CGMCC No.18658. During cultivation, the biocontrol streptomycete is inoculated to a fermentation medium for fermentation cultivation. The strain or a biocontrol product prepared through the strain can be used for plant pathogen preventing and treating. The biocontrol streptomycete or a biocontrol preparation prepared through the biocontrol streptomycete has the advantages of good antibacterial effect, broad antimicrobial spectrum, low toxicity, no residue, environmental friendliness, broad development prospect and the like.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a biocontrol streptomyces. Background technique [0002] Blueberry, also known as lingonberry, belongs to the plant of the genus Vaccinium Spp. in the family Ericaceae. Its fruit has super high nutritional value. Besides fructose and vitamins, it is rich in a large amount of anthocyanins, various antioxidants and flavonoids. Its unique and rare nutritional and health value is more and more popular among people. The demand for blueberries is constantly growing. [0003] In recent years, blueberry planting area has increased year by year, and blueberry diseases have become more and more serious, causing heavy economic losses. Blueberry canker is an important disease of blueberries caused by Botryosphaeria dothidea. The disease is widely distributed in the world, and mainly damages one-year-old branches, mostly from wounds and pruning openings; reddish-brown lesions a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P1/00C12R1/465
CPCA01N63/28
Inventor 李立梅丁芮涵陈越渠左彤彤于海媛孙伟刘庆珍王向东李刚
Owner JILIN CITY ACADEMY OF FORESTRY
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