Multichannel biological reaction device provided with microfluidic or nanofluidic structure

Abstract

The invention relates to a multichannel biological reaction device provided with a microfluidic or nanofluidic structure. The device comprises the following structures: a biological sample extractionbin, at least two reaction bins and a microfluidic or nanofluidic structure, wherein the microfluidic or nanofluidic structure comprises a biological sample channel and a plurality of reaction liquidinjection channels; a biological sample is transferred into each reaction bin from the biological sample extraction bin through the biological sample channel; each reaction bin is communicated with one of the reaction liquid injection channels; the microfluidic or nanofluidic structure comprises a plurality of first valves and a plurality of second valves; each first valve is used for opening or blocking the biological sample channel; and each second valve is used for opening or blocking the reaction liquid injection channel. According to the biological reaction device, the biological sample can be accurately quantified and sufficiently and efficiently transferred into the reaction bins, the experiment process is simplified, the experiment efficiency is improved, and the possibility that the biological sample is polluted during transferring is reduced.

Description

technical field [0001] The invention relates to the technical fields of biochemistry and molecular biology, in particular to a multi-channel biological reaction device provided with a microfluidic or nanofluidic structure. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) an...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

[0004] There are some biological reaction devices on the market, but these devices generally can only perform one test, not only the efficiency is low, but each test generally needs to reconfigure the samples, and the different samples configured cannot be guaranteed to be exactly the same. Therefore, there are differences between the test results, and serious cases may lead to the failure of the experiment

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