Aqueous extract powder of fresh celery leaves, preparation thereof and vasodilation effect and application of aqueous extract powder of fresh celery leaves

A kind of water extract, powder technology, applied in fresh celery leaf water extract powder, its preparation, vasodilatation effect and application field

Inactive Publication Date: 2020-01-21
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two fatal problems make the inventor have to give up the follow-up development of fresh celery leaf nano juice

Method used

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  • Aqueous extract powder of fresh celery leaves, preparation thereof and vasodilation effect and application of aqueous extract powder of fresh celery leaves
  • Aqueous extract powder of fresh celery leaves, preparation thereof and vasodilation effect and application of aqueous extract powder of fresh celery leaves
  • Aqueous extract powder of fresh celery leaves, preparation thereof and vasodilation effect and application of aqueous extract powder of fresh celery leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 prepares fresh celery leaf aqueous extract powder

[0025] 1000g fresh celery leaves with no sediment on the surface were washed twice with 3000mL tap water, then transferred to a sterile environment and washed three times with 3000mL triple-distilled water. Strain off the water to get clean fresh celery leaves. Soak clean fresh celery leaves in triple-distilled water at 60°C-90°C for 3 times, each time soaking in 3000mL triple-distilled water for at least 30 minutes, stirring slowly during soaking. After each soaking, let the water extract flow out slowly by itself, and it takes no less than 30 minutes. Combine about 9000mL of water extracts that flow out slowly, and concentrate under reduced pressure at 60°C-90°C to obtain 24g of fresh celery leaf water extract powder.

Embodiment 2

[0026] Embodiment 2 measures the chromatogram and mass spectrometry ion flow spectrum of fresh celery leaf aqueous extract powder

[0027] 2-1. Preparation of sample solution (10mg / mL)

[0028] Weigh 26.7mg fresh celery leaf aqueous extract powder, and dissolve the powder in 2.67mL ultrapure water. The obtained solution was ultrasonically shaken for 1 minute, and then centrifuged at 13000r / min for 10 minutes. Take the supernatant, pass through a 0.22μm filter membrane, and place it in a sample bottle for chromatography and mass spectrometry.

[0029] 2-2. Chromatographic conditions

[0030] Chromatographic column: Waters, Acquity HSS T3 column (2.1×100mm i.d., 1.8μm);

[0031] Injection volume: 2μL;

[0032] PDA detector: 210-400nm;

[0033] Mobile phase: water (0.1% formic acid), acetonitrile;

[0034] The mobile phase washes the column according to the gradient in Table 1.

[0035] Table 1 Mobile phase gradient table

[0036]

[0037] 2-3. Measurement chromatog...

Embodiment 3

[0049] Embodiment 3 specifies the structure of 34 components in the fresh celery leaf aqueous extract powder

[0050] The UPLC chromatography of Example 2 was coupled with mass spectrometry, and the UPLC-mass spectrometry of fresh celery leaf aqueous extract powder was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive ion and negative ion. The slope well collision energy is 20-60V. Garden cone voltage is 40V. The ion source temperature was 100°C. The capillary voltage was 30 kV. The pressure of the nebulizer is 6.5 bar. The mass / charge ratio ranges from 100-1500. 34 independent peaks resolved within 14 minutes. According to the fragmentation law of mass spectrometry, these peaks (the peaks of the total ion current spectrum are numbered sequentially from left to right) are designated as the corresponding components in Table 2.

[0051] Table 2 The component names corresponding to the peaks of the total ion current spectrum

...

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Abstract

The invention discloses a preparation method of aqueous extract powder of fresh celery leaves. The invention discloses structure types and names of all 34 components in the prepared aqueous extract powder, and discloses that the 34 components are all novel compounds, wherein in the 34 novel compounds, 3 compounds are sucrose type compounds, 13 compounds are quinic acid type compounds, and 18 compounds are apiin type compounds. The invention discloses an effect of the aqueous extract powder of fresh celery leaves in vasodilation, and therefore discloses application of the aqueous extract powderof fresh celery leaves in preparation of vasodilating drugs.

Description

technical field [0001] The present invention relates to the method for preparing powder from the water extract of fresh celery leaves, relates to the reproducibility of the process of preparing powder from the water extract of fresh celery leaves by its characteristic UPLC-mass spectrum total ion current pattern and its 34 The components in the powder are defined by its characteristic UPLC-MS total ion chromatogram and its 34 peaks, and the 34 peaks correspond to the three sucrose structures in the fresh celery leaf water extract powder, involving 34 13 peaks correspond to 13 quinic acid structures in fresh celery leaf water extract powder, 34 peaks correspond to 18 apigenin structures in fresh celery leaf water extract powder, and further involve fresh celery leaf water extract powder Therefore, the present invention relates to the application of fresh celery leaf aqueous extract powder in the preparation of vasoconstriction disease prevention medicine. The invention belongs...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/04C07H13/04C07H17/07C07C67/48C07C69/732C07C69/734A61P9/08A61K36/23G01N30/02G01N30/06
CPCA61K36/23A61P9/08C07C67/48C07H1/08C07H13/04C07H17/04C07H17/07G01N30/02G01N30/06C07C69/732C07C69/734
Inventor 彭师奇赵明黄飞
Owner 北京恒润泰生医药科技有限公司
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