Method for extracting and separating polysaccharide from codium fragile

A technology of pine algae and polysaccharides, applied in the direction of antiviral agents, etc., can solve the problems of difficult separation and low extraction rate of polysaccharides, and achieve the effect of large adsorption capacity, low viscosity of slurry, and easy filtration and separation

Inactive Publication Date: 2020-01-24
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems of low extraction rate and difficult separation of polysaccharides extracted from P. spinosa, the present invention provides a method for extracting and separating polysaccharides from P. spinosa. Water passes through fatty acid ether sodium sulfate and polysorbate-8

Method used

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  • Method for extracting and separating polysaccharide from codium fragile
  • Method for extracting and separating polysaccharide from codium fragile
  • Method for extracting and separating polysaccharide from codium fragile

Examples

Experimental program
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Example Embodiment

[0022] Example 1

[0023] 1) Dry the Acanthophyllum at 40 ℃ and crush it through a 60-mesh sieve for later use. Take pine algae powder, reflux with 85% ethanol for defatting for 2 h, repeat twice and then dry to obtain defatted pine algae powder. Then proceed to cell wall destruction treatment: heat a small amount of water to 50 ℃, add 100g of defatted pine algae powder and stir, add water in batches until the defatted pine algae powder just dissolves, 0.5 g of fatty acid ether sodium sulfate and 5 g of polysorbate- 80 is dissolved in ethanol with a volume of 5% water volume, the ethanol solution is added to the defatted pine algae powder aqueous solution, stirred vigorously for 2 h, centrifuged to take the water phase, filtered to take the filter cake, and dried for later use;

[0024] 2) Add about 20 times the volume of water to the dried Acanthopanax sinensis, stir and extract for 2 h with an electronic mixer in a water bath at 100 ℃, centrifuge, and repeat the extraction of th...

Example Embodiment

[0030] Example 2

[0031] 1) Take the defatted pine algae powder of Example 1 for cell wall destruction treatment: heat a small amount of water to 70 ℃, add 100 g of defatted pine algae powder and stir, add water in batches until the defatted pine algae powder just dissolves, add 2 g fatty acid ether sodium sulfate and 3 g polysorbate-80 are dissolved in ethanol with a volume of 20% water volume, and the ethanol solution is added to the defatted pine algae powder aqueous solution, stirred vigorously for 3 h, centrifuged to take the water phase, filtered to take the filter cake , Dry and reserve;

[0032] 2) Add about 30 times the volume of water to the dried Acanthopanax sinensis, stir and extract for 4 h with an electronic mixer in a water bath at 80 ℃, centrifuge, and repeat the extraction of the algae residue twice, combine the supernatants, concentrate and precipitate with alcohol, and place in a refrigerator at 9 ℃ Let it stand overnight in the medium, filter with suction, ad...

Example Embodiment

[0035] Example 3

[0036] Take the defatted pine algae powder of Example 1 for cell wall destruction treatment: heat a small amount of water to 60 ℃, add 100 g of defatted pine algae powder and stir, add water in batches until the defatted pine algae powder just dissolves, add 1 g Fatty acid ether sodium sulfate and 4 g of polysorbate-80 are dissolved in ethanol with a volume of 10% by volume of water. The ethanol solution is added to the defatted Pine Algae powder aqueous solution, stirred vigorously for 3 h, centrifuged to take the water phase, and filtered to take the filter cake. Dry and reserve;

[0037] 2) Add about 20 times the volume of water to the dried Acanthopanax sinensis, stir and extract for 1 h with an electronic mixer in a water bath at 90 ℃, centrifuge, and repeat the extraction of the algae residue twice, combine the supernatants and concentrate after alcohol precipitation, and place in a refrigerator at 3 ℃ Let stand overnight in the medium, filter with suction...

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Abstract

The invention relates to the technical field of polysaccharide extraction. Aiming at solving the problems of low polysaccharide extraction rate from codium fragile and difficult separation, the invention provides a method for extracting and separating polysaccharide from codium fragile. The method comprises the following steps: performing pretreatment comprising degreasing on codium fragile; thenperforming extracting on the codium fragile with hot water, performing alcohol precipitation, performing standing overnight, performing suction filtration, adding ethanol for washing, and carrying outdehydrating and drying to obtain a crude polysaccharide which is extracted from the codium fragile with hot water; and finally separating the crude polysaccharide which is extracted from the codium fragile with hot water by using a strong anion exchange chromatographic column: concretely performing gradient elution with an NaCl solution, collecting the polysaccharide by a fraction collector, performing pressure-reduced concentration on the eluent of the components, performing dialysis for desalination, and performing freeze drying to obtain the product polysaccharide. According to the method,cell wall rupture and polysaccharide release are greatly promoted through fatty acid ether sodium sulfate and polysorbate-80 active polypeptide in advance, so that the extraction time is reduced, thepolysaccharide extraction rate is reduced, and the polysaccharide with anti-HPV activity is prepared through proper gradient elution in the strong anion exchange chromatographic column.

Description

technical field [0001] The invention relates to the technical field of extracting polysaccharides, in particular to a method for extracting and separating polysaccharides from P. chinensis. Background technique [0002] Echinococcus is a perennial green algae belonging to the Chlorophyta. The algae are dendritic, mostly staghorn-shaped branches, cylindrical branches, and a few are flat. The interior is a sponge-shaped structure composed of intertwined tubular filaments, and the surface has closely arranged filaments to form a club-shaped capsule. The extraction of macromolecules containing sugars from P. chinensis has been studied for a long time. For example, love J et al. proved that the water extract of the algae contains glucan, mannan, highly sulfated and pyruvated galactan and Sulfated arabinan. However, the separation and purification of the extracted polysaccharides is still a difficult problem. In addition, the cell wall of this seaweed is a highly assembled stru...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/20
CPCA61P31/20C08B37/0003C08B37/006
Inventor 陈荫王腾董喆孙坤来赵玉勤王斌
Owner ZHEJIANG OCEAN UNIV
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