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Fluorescent probe for detecting content of water in heavy water and application of fluorescent probe

A technology of fluorescent probes and heavy water, applied in measuring devices, fluorescence/phosphorescence, luminescent materials, etc., can solve the problems of human harm, achieve the effects of low preparation cost, simple and easy synthesis process, and easy promotion

Inactive Publication Date: 2020-01-24
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although heavy water has an important use value, it is harmful to humans. Microorganisms and fish will die in pure heavy water or water containing more heavy water (more than 80%) within a few hours.

Method used

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  • Fluorescent probe for detecting content of water in heavy water and application of fluorescent probe
  • Fluorescent probe for detecting content of water in heavy water and application of fluorescent probe
  • Fluorescent probe for detecting content of water in heavy water and application of fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Synthesis of Fluorescent Probes

[0024]

[0025] Take compound 1 (80 mg, 0.2 mmol), 7-hydroxycoumarin (40 mg, 0.2 mmol), HOBT (13.5 mg, 0.1 mmol), EDC (78 mg, 0.4 mmol) in 4 mL DMF and at room temperature Stir for 10 minutes, then add 200 μL DIEA and continue stirring for 5 hours under nitrogen protection, then pass it through column chromatography, and change to CH 2 Cl 2 : CH 3 OH = 10:1 (v / v) for purification to obtain an orange-red fluorescent probe with a yield of 65%, 1 H NMR spectrum see figure 1 .

Embodiment 2

[0026] Example 2 Fluorescent detection of heavy water with different light water contents by fluorescent probe

[0027] Prepare in advance 5 mL of a 10 μM probe heavy solution in 0.33% DMSO containing 10% to 100% light water. Fluorescence detection is then performed (λ Ex = 400 nm); calculate the fluorescence intensity in each system; by analyzing the relationship between the fluorescence intensity at 450 nm and 550 nm and the light water content, evaluate the response performance of the probe to the light water content (see figure 2 , image 3 with Figure 4 ). figure 2 , image 3 with Figure 4 It shows that with the increase of light water content, the fluorescence intensity ratio I of the solution 450 and I 550 Gradually decreased, while light water content and fluorescence intensity showed a good linear relationship.

Embodiment 3

[0028] Example 3 Selectivity of fluorescent probes to different ions

[0029] Prepare 5 mL of 10 μM probe heavy aqueous solution containing 0.33% DMSO in advance, and then add 50 μL of CuSO with a concentration of 200 μM to the system 4 、Na 2 S, Na 2 SO 3 、H 2 o 2 , Cys, GSH, NaNO 2 Aqueous solutions of small molecules. Fluorescence detection is then performed (λ Ex = 400 nm); calculate the fluorescence intensity I in each system 550 and I 440 ; Assess the interference of the different substances on the fluorescent probe solution (see Figure 5 ). As can be seen from the figure, of course adding CuSO to the probe solution 4 、Na 2 For small molecules such as S, the fluorescence of the solution basically does not change.

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Abstract

The invention provides a fluorescent probe for detecting content of light water in heavy water: a formula shown in the description. According to the fluorescent probe provided by the invention, hydroxyl groups of coumarin and xanthene parts serve as response loci on the basis of different pH values of the heavy water and the light water. The fluorescent probe for detecting the content of the lightwater in the heavy water, provided by the invention, can be obtained through chemical synthesis, the synthesis process is easy and feasible, raw materials are cheap and readily available, the preparation cost is low, and thus, popularization is easy. The probe has high sensitivity, is basically free from interference caused by other ingredients during detecting on the content of the light water in corresponding heavy water and has a broad application prospect. The probe has good fluorescence-emission spectral characteristics and can achieve the aim of rapidly and accurately detecting the content of the light water in the heavy water.

Description

technical field [0001] The invention belongs to the field of organic small molecule fluorescent probes, in particular to a fluorescent probe for detecting water content in heavy water based on the structures of coumarin and xanthene. Background technique [0002] Deuterium oxide is a compound composed of deuterium and oxygen, molecular formula D 2 O, relative molecular mass 20.0275, than water (H 2 O) has a molecular weight of 18.0153 about 11% higher, so it is called heavy water. In natural water, the content of heavy water is about 0.015%. Since the properties of deuterium and hydrogen differ very little, heavy water is similar to ordinary water. [0003] The special value of heavy water is reflected in the application of atomic energy technology. To manufacture powerful nuclear weapons, heavy water is needed as a moderator in the nuclear fission reaction. When using NMR analysis, if the solvent is water and the research object is hydrogen, heavy water can be used as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07D493/10G01N21/64
CPCC07D493/10C09K11/06C09K2211/1044C09K2211/1088G01N21/643
Inventor 林伟英董宝利卢雅如宋文辉孙亚茹
Owner UNIV OF JINAN
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