An isotope mass spectrometry sampling system for studying photosynthetic gas exchange

Abstract

The invention discloses an isotope mass spectrum sample injection system for researching photosynthetic gas exchange. The system comprises a photosynthesis leaf chamber simulation device, a cold trapand an isotope mass spectrometer, the photosynthesis leaf chamber simulation device comprises a temperature control base, a light channel, a gas channel, a photosynthesis simulator and a semipermeablemembrane. The isotope mass spectrum sample injection system for researching photosynthetic gas exchange disclosed by the invention designs the photosynthesis leaf chamber simulation device in a laboratory environment by simulating the photosynthesis of green plants, and artificially and controllably delivers O2, CO2, H2O and light to green lamina, enables a mixed gas composed of O2 and CO2 to selectively pass through by using the semipermeable membrane, purifies the mixed gas by using the cold trap, and finally detects the mixed gas by using the isotope mass spectrometer, change trajectoriesof carbon and oxygen isotopes during photosynthesis gas ingress and egress can be automatically and continuously monitored online, and the dynamic process of carbon assimilation in a natural state isstudied.

Description

technical field

[0001] The invention relates to the technical field of isotope tracer, in particular to an isotope mass spectrometry sampling system for studying photosynthetic gas exchange. Background technique

[0002] Photosynthesis is the use of light energy by green plants to convert CO 2 and H 2 O synthesizes organic matter while releasing O 2 physiological process. The general process is as follows: First, the CO 2 Diffusion from the outside atmosphere to the leaf surface; then, through the stomata to the substomatal cavity; finally, to the chloroplast carboxylation site for assimilation by carboxylase. However, plant photosynthesis is very inefficient, converting only about 3% of solar energy into usable form.

[0003] Isotope tracer technology has the characteristics of fast detection and accurate results. Its application involves many ecological research fields and has become one of the most effective research methods in the field of ecology and environmental ...

Examples

Embodiment 1

[0020] Embodiment 1 provides an isotope mass spectrometry sampling system for studying photosynthetic gas exchange, and its structure will be described in detail below.

[0021] refer to figure 1 , the isotope mass spectrometry sampling system comprises a photosynthetic leaf chamber simulation device 1, a cold trap 2 and an isotope mass spectrometer 3 which are sequentially connected by pipelines from front to back.

[0022] refer to figure 2 , 3 The photosynthetic lobe chamber simulation device 1 includes a temperature control base 10 , an optical channel 11 , a gas channel 12 , a photosynthetic simulator 13 , and a semipermeable membrane 14 . Wherein, the temperature control base 10 is provided with a groove, and the photosynthesis simulator 13 is arranged in the groove of the temperature control base 10; the semipermeable membrane 14 is arranged on the bottom surface of the photosynthesis simulator 13 and between the surfaces of the grooves of the temperature control ba...

Embodiment 2

[0038] Embodiment 2 provides a kind of isotope mass spectrometry analysis method of photosynthetic gas exchange, adopts the isotope mass spectrometry system that embodiment 1 provides, and this analysis method comprises the following steps:

[0039] Step S1: First place the photosynthetic sample (leaf) on the support net 16, then assemble the photosynthetic leaf chamber simulation device 1, pass light from the light channel 11, and the air inlet 121 of the gas channel 12 receives CO 2 and O 2 ;

[0040] Step S2: CO 2 and H 2 O undergoes photosynthesis in the leaf chamber 130, and produces O 2 ;

[0041] Step S3: O in the leaf chamber 130 2 , CO 2 and trace H 2 The mixed gas composed of O diffuses through the semi-permeable membrane 14;

[0042] Step S4: The mixed gas enters the cold trap 2 to remove trace moisture in the mixed gas;

[0043] Step S5: The mixed gas enters the isotope mass spectrometer 3 .

technical field

[0001] The invention relates to the technical field of isotope tracer, in particular to an isotope mass spectrometry sampling system for studying photosynthetic gas exchange. Background technique

[0002] Photosynthesis is the use of light energy by green plants to convert CO 2 and H 2 O synthesizes organic matter while releasing O 2 physiological process. The general process is as follows: First, the CO 2 Diffusion from the outside atmosphere to the leaf surface; then, through the stomata to the substomatal cavity; finally, to the chloroplast carboxylation site for assimilation by carboxylase. However, plant photosynthesis is very inefficient, converting only about 3% of solar energy into usable form.

[0003] Isotope tracer technology has the characteristics of fast detection and accurate results. Its application involves many ecological research fields and has become one of the most effective research methods in the field of ecology and environmental ...

Examples

Embodiment 1

[0020] Embodiment 1 provides an isotope mass spectrometry sampling system for studying photosynthetic gas exchange, and its structure will be described in detail below.

[0021] refer to figure 1 , the isotope mass spectrometry sampling system comprises a photosynthetic leaf chamber simulation device 1, a cold trap 2 and an isotope mass spectrometer 3 which are sequentially connected by pipelines from front to back.

[0022] refer to figure 2 , 3 The photosynthetic lobe chamber simulation device 1 includes a temperature control base 10 , an optical channel 11 , a gas channel 12 , a photosynthetic simulator 13 , and a semipermeable membrane 14 . Wherein, the temperature control base 10 is provided with a groove, and the photosynthesis simulator 13 is arranged in the groove of the temperature control base 10; the semipermeable membrane 14 is arranged on the bottom surface of the photosynthesis simulator 13 and between the surfaces of the grooves of the temperature control ba...

Embodiment 2

[0038] Embodiment 2 provides a kind of isotope mass spectrometry analysis method of photosynthetic gas exchange, adopts the isotope mass spectrometry system that embodiment 1 provides, and this analysis method comprises the following steps:

[0039] Step S1: First place the photosynthetic sample (leaf) on the support net 16, then assemble the photosynthetic leaf chamber simulation device 1, pass light from the light channel 11, and the air inlet 121 of the gas channel 12 receives CO 2 and O 2 ;

[0040] Step S2: CO 2 and H 2 O undergoes photosynthesis in the leaf chamber 130, and produces O 2 ;

[0041] Step S3: O in the leaf chamber 130 2 , CO 2 and trace H 2 The mixed gas composed of O diffuses through the semi-permeable membrane 14;

[0042] Step S4: The mixed gas enters the cold trap 2 to remove trace moisture in the mixed gas;

[0043] Step S5: The mixed gas enters the isotope mass spectrometer 3 .

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