Drug synergist for preventing and treating mythimna separata and using method thereof
A synergist and armyworm technology, applied in botany equipment and methods, insecticides, animal repellants, etc., can solve the problems of no discovery, easy generation of cross-resistance, unavoidable environment and crops, etc., to achieve The effect of improving the control effect
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[0019] Embodiment: A kind of drug synergist for preventing and controlling armyworm and its application method
[0020] 1.1 Armyworms tested
[0021] Armyworm 2nd instar larvae.
[0022] 1.2 Concentration ratio of cyclosporine A and spinosad
[0023] The concentration of spinosyn was 0.5 mg / L, which was mixed with different concentrations of cyclosporine A (50 μM and 100 μM).
[0024] 1.3 Bioassay methods
[0025] What this application adopts is medicament mixed artificial feed (yeast powder 34 g, wheat germ powder 40 g, corn leaf powder 100 g, mix well; 2, sorbic acid 2 g, methylparaben 4 g, 300 mL distilled water boil and mix After uniformity, add the mixture into ① and mix well; 3. 600 mL distilled water, 18 g agar powder, boil and mix well, add to ②, stir well; 4. When the temperature drops to 40°C, add VC 2.5g, sub 5-6 drops of oleic acid (soybean oil), mix well; store at 5, 4°C.) for bioassay. That is, the armyworm artificial diet was prepared first, and spinosad or...
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