Trace MUC1 fluorescence detection method based on chained hybridization reaction and fluorescence carbon quantum dots

Abstract

The invention discloses a trace MUC1 fluorescence detection method based on the chained hybridization reaction and fluorescence carbon quantum dots. The method comprises the steps that carbon quantumdots are synthesized, neck ring DNA H1 and H2 are modified to the carbon quantum dots, and the carbon quantum dots are mixed with graphene oxide; when MUC1 does not exist, the carbon quantum dots modified with H1 and H2 are adsorbed to the surface of the graphene oxide, and because of the fluorescence resonance energy transfer effect, fluorescence quenching happens to the carbon quantum dots; whenMUC1 exists, MUC1 is combined with aptamer, chained hybridization reaction is promoted to happen to H1 and H2 to form chained hybrid products, H1 and H2 get away from the surface of graphene, and finally, the carbon quantum dots recover fluorescence; and then, the content of MUC1 can be determined through fluorescence detection. According to the trace MUC1 fluorescence detection method based on the chained hybridization reaction and fluorescence carbon quantum dots, signal amplification is achieved through chained hybridization reaction, the sensitivity of MUC1 detection is greatly improved;and furthermore, the fluorescent carbon quantum dots are stable in fluorescence property, and the stability and the repeatability of the method are greatly improved.

Description

technical field [0001] The invention relates to the field of trace MUC1 detection, in particular to a trace MUC1 fluorescence detection method based on chain hybridization reaction and fluorescent carbon quantum dots. Background technique [0002] Mucin (MUC1) is a class of high molecular weight, highly glycosylated proteins that exist in epithelial cells and form a complete transmembrane domain through a gel matrix. Mucin (MUC1) is one of the cell surface glycoproteins. Its polypeptide backbone consists of three parts: extracellular segment, transmembrane segment and intracellular segment. The transmembrane segment and intracellular segment contain 31 and 69 amino acids respectively; The stretch contains multiple contiguous repeats, each repeat containing 20 amino acids. MUC1 plays an important role in the signal transduction process. In addition, MUC1 can down-regulate the expression of E-cadherin, which is a calcium ion-dependent cell adhesion molecule that mediates inte...

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Problems solved by technology

However, the traditional method faces many problems such as low sensitivity and complicated operation.

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