Preparation method and application of nanocomposite material and electrochemical microrna biosensor
A nanocomposite material and biosensor technology, applied in scientific instruments, analytical materials, material analysis through electromagnetic means, etc., can solve the problems of small electrical signal, short microRNA sequence, low abundance, etc., to increase conductivity, method Simple, Accessible Effects
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Embodiment 1
[0045] Embodiment 1 Nanocomposite C of the present invention 60 Preparation of @PAMAM-MOF and Tracer label 1
[0046] Weigh 0.187g FeCl 3 ·6H 2 O and 0.126g of diaminoterephthalic acid were dissolved in 15mL of DMF solution, and then 207μL of acetic acid was added for ultrasonic treatment to make it evenly mixed; the above mixed solution was transferred to the reaction bottle and kept stirring in 120°C silicone oil for 4h, and the reaction was cooled After reaching room temperature, the MOF was obtained by centrifuging and washing with DMF and ethanol three times to remove excess solvent.
[0047] Weigh 5 mg of the MOF prepared above and dissolve it in 5 mL of ultrapure water to obtain a brown-red solution. Add 200 μL PAMAM solution and the color immediately changes from brown-red to bright yellow. After stirring overnight at room temperature, wash with ultra-pure water for 3 times at 10,000 rpm for 8 minutes to remove unbound PAMAM, dispersed in ultrapure water to obtain P...
Embodiment 2
[0055] Embodiment 2 Nanocomposite material NC of the present invention 60 Preparation of @PAMAM-MOF and Tracer label 2
[0056] 5mL C prepared in Comparative Example 1 60 @PAMAM solution was added to MOF solution (1mg mL -1 ) at room temperature for 30 hours, washed with ultrapure water for 3 times and then dispersed into ultrapure water to obtain NC 60 @PAMAM-MOF.
[0057] Using NC 60 @PAMAM-MOF material preparation of tracer label (Tracer label): The method is the same as the preparation of Tracer label in Example 1, and the prepared tracer label (Tracer label) is recorded as Tracer label2.
[0058] figure 2 Specifically showing the NC of the present invention 60 @PAMAM-MOF preparation process and corresponding Tracer label preparation process. like figure 2 As shown, in the direct stirring method to prepare NC 60 @PAMAM - MOF process, C 60 into a needle-like structure.
Embodiment 3
[0059] Embodiment 3 Construction of the electrochemical microRNA biosensor of the present invention
[0060] Preparation of CS-AB dispersion: Weigh 1 mg of acetylene black powder and add it to the existing chitosan acetic acid solution, and ultrasonically treat it to obtain a chitosan-acetylene black (CS-AB) dispersion, which is set aside.
[0061] Preparation of Pt@AuNPs: 1mL 1%HAuCl 4Add the solution to 100mL ultrapure water and boil, then quickly add 2.5mL 1% trisodium citrate solution and continue to boil for 15 minutes. After cooling, use ultrapure water to return to the original volume to obtain a transparent wine red solution that is gold nanoparticles (AuNPs). . Add 20 mL of AuNPs to 30 mL of ultrapure water and heat to 80 °C, then quickly add 2.5 mL of 1% H 2 PtCl 6 The solution was vigorously stirred, and then 1.75mL of 1% AA was added dropwise. The solution quickly turned black and continued to heat and reflux until the color did not change. Stop heating and cool...
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